Capsid opening enables genome release of iflaviruses.

ŠKUBNÍK, Karel, Lukáš SUKENÍK, David BUCHTA, Tibor FÜZIK, Michaela PROCHÁZKOVÁ, Jana MORAVCOVÁ, Lenka ŠMERDOVÁ, Antonín PŘIDAL, Robert VÁCHA and Pavel PLEVKA. Capsid opening enables genome release of iflaviruses. Science advances. New York: American Association for the Advancement of Science, 2021, vol. 7, No 1, p. 1-9. ISSN 2375-2548. Available from: https://dx.doi.org/10.1126/sciadv.abd7130.

Basic information

Original name

Capsid opening enables genome release of iflaviruses.

Authors

ŠKUBNÍK, Karel (203 Czech Republic, belonging to the institution), Lukáš SUKENÍK (203 Czech Republic, belonging to the institution), David BUCHTA (203 Czech Republic, belonging to the institution), Tibor FÜZIK (703 Slovakia, belonging to the institution), Michaela PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Jana MORAVCOVÁ (203 Czech Republic, belonging to the institution), Lenka ŠMERDOVÁ (203 Czech Republic, belonging to the institution), Antonín PŘIDAL (203 Czech Republic), Robert VÁCHA (203 Czech Republic, belonging to the institution) and Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Science advances, New York, American Association for the Advancement of Science, 2021, 2375-2548

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10607 Virology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 14.957

RIV identification code

RIV/00216224:14740/21:00118990

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1126/sciadv.abd7130

UT WoS

000605159200031

Keywords in English

DEFORMED WING VIRUSBEE PARALYSIS VIRUSCRYO-EMMOLECULAR-DYNAMICSFORCE-FIELDX-RAYPOLIOVIRUSRNAMODELTRANSITIONS

Tags

CF BIC, CF CRYO, CF PROT, rivok

Tags

International impact, Reviewed

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Abstract

V originále

The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions and capsids organized with icosahedral symmetry. The genome release of iflaviruses can be induced in vitro by exposure to acidic pH, implying that they enter cells by endocytosis. Genome release intermediates of iflaviruses have not been structurally characterized. Here, we show that conformational changes and expansion of iflavirus RNA genomes, which are induced by acidic pH, trigger the opening of iflavirus particles. Capsids of slow bee paralysis virus and sacbrood virus crack into pieces. In contrast, capsids of deformed wing virus are more flexible and open like flowers to release their genomes. The large openings in iflavirus particles enable the fast exit of genomes from capsids, which decreases the probability of genome degradation by the RNases present in endosomes.

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Links

EF16_013/0001776, research and development project	Name: Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví
GX19-25982X, research and development project	Name: Analýza replikace enterovirů s využitím elektronové mikroskopie Investor: Czech Science Foundation
LL2007, research and development project	Name: Peptidoví zabijáci bakterií (Acronym: PeptideKillers) Investor: Ministry of Education, Youth and Sports of the CR
LM2015085, research and development project	Name: CERIT Scientific Cloud (Acronym: CERIT-SC) Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud
LM2018140, research and development project	Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ) Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project	Name: CEITEC 2020 (Acronym: CEITEC2020) Investor: Ministry of Education, Youth and Sports of the CR
90127, large research infrastructures	Name: CIISB II