The molecular epidemiology of methicillin-resistant
Staphylococcus aureus (MRSA) in the Czech Republic

J 2021

The molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in the Czech Republic

TKADLEC, Jan, Vaclav CAPEK, Marie BRAJEROVA, Eva SMELIKOVA, Oto MELTER et. al.

Basic information

Original name

The molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in the Czech Republic

Authors

TKADLEC, Jan (203 Czech Republic, guarantor), Vaclav CAPEK (203 Czech Republic), Marie BRAJEROVA (203 Czech Republic), Eva SMELIKOVA (203 Czech Republic), Oto MELTER (203 Czech Republic), Tamara BERGEROVA (203 Czech Republic), Sylvia POLIVKOVA (203 Czech Republic), Magda BALEJOVA (203 Czech Republic), Marketa HANSLIANOVA (203 Czech Republic), Daniela FACKOVA (203 Czech Republic), Katerina NERADOVA (203 Czech Republic), Renata TEJKALOVÁ (203 Czech Republic, belonging to the institution), Iva VAGNEROVA (203 Czech Republic), Natasa BARTONIKOVA (203 Czech Republic), Eva CHMELAROVA (203 Czech Republic), Pavel DREVINEK (203 Czech Republic) and Marcela KRUTOVA (203 Czech Republic)

Edition

Journal of Antimicrobial Chemotherapy, OXFORD, Oxford University Press, 2021, 0305-7453

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.758

RIV identification code

RIV/00216224:14110/21:00121695

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1093/jac/dkaa404

UT WoS

000620811500006

Keywords in English

molecular epidemiology; methicillin-resistant Staphylococcus aureus

Abstract

V originále

Objectives: To gain data on the current molecular epidemiology and resistance of MRSA in the Czech Republic. Methods: Between September 2017 and January 2018, a total of 441 single-patient MRSA isolates were collected from 11 Czech hospitals and analysed by spa typing, SCCmec typing, antibiotic susceptibility testing, detection of the PVL toxin and the arcA gene. Results: Of all MRSA isolates, 81.41% (n = 359) belonged to the CC5-MRSA clone represented by the spa types t003 (n = 136), t586 (n = 92), t014 (n = 81), t002 (n = 20) and other spa types (n = 30); a majority of the CC5 isolates (n = 348, 96.94%) carried SCCmec type II. The occurrence of CC5-MRSA was more Likely in older inpatients and associated with a healthcare origin (P< 0.001). The CC5-MRSA isolates were resistant to more antimicrobial drugs compared with the other MRSAs (P< 0.001). Interestingly, t586 was detected in blood samples more often than the other spa types and, contrary to other spa types belonging to CC5-MRSA, t586 was not associated with patients of advanced age. Other frequently found Lineages were CC8 (n = 17), CC398 (n = 11) and CC59 (n = 10). The presence of the PVL was detected in 8.62% (n = 38) of the MRSA isolates. Conclusions: The healthcare-associated CC5-MRSA-II Lineage (t003, t586, t014) was found to be predominant in the Czech Republic. t586 is a newly emerging spa type in the Czech Republic, yet reported rarely in other countries. Our observations stress the need for MRSA surveillance in the Czech Republic in order to monitor changes in MRSA epidemiology.

Citovat

TKADLEC, Jan, Vaclav CAPEK, Marie BRAJEROVA, Eva SMELIKOVA, Oto MELTER, Tamara BERGEROVA, Sylvia POLIVKOVA, Magda BALEJOVA, Marketa HANSLIANOVA, Daniela FACKOVA, Katerina NERADOVA, Renata TEJKALOVÁ, Iva VAGNEROVA, Natasa BARTONIKOVA, Eva CHMELAROVA, Pavel DREVINEK and Marcela KRUTOVA. The molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in the Czech Republic. Journal of Antimicrobial Chemotherapy. OXFORD: Oxford University Press, 2021, vol. 76, No 1, p. 55-64. ISSN 0305-7453. Available from: https://dx.doi.org/10.1093/jac/dkaa404.

@article{1773201,
   author = {Tkadlec, Jan and Capek, Vaclav and Brajerova, Marie and Smelikova, Eva and Melter, Oto and Bergerova, Tamara and Polivkova, Sylvia and Balejova, Magda and Hanslianova, Marketa and Fackova, Daniela and Neradova, Katerina and Tejkalová, Renata and Vagnerova, Iva and Bartonikova, Natasa and Chmelarova, Eva and Drevinek, Pavel and Krutova, Marcela},
   article_location = {OXFORD},
   article_number = {1},
   doi = {http://dx.doi.org/10.1093/jac/dkaa404},
   keywords = {molecular epidemiology; methicillin-resistant Staphylococcus aureus},
   language = {eng},
   issn = {0305-7453},
   journal = {Journal of Antimicrobial Chemotherapy},
   title = {The molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in the Czech Republic},
   url = {https://academic.oup.com/jac/article-abstract/76/1/55/5942696?redirectedFrom=fulltext},
   volume = {76},
   year = {2021}
}

TY  - JOUR
ID  - 1773201
AU  - Tkadlec, Jan - Capek, Vaclav - Brajerova, Marie - Smelikova, Eva - Melter, Oto - Bergerova, Tamara - Polivkova, Sylvia - Balejova, Magda - Hanslianova, Marketa - Fackova, Daniela - Neradova, Katerina - Tejkalová, Renata - Vagnerova, Iva - Bartonikova, Natasa - Chmelarova, Eva - Drevinek, Pavel - Krutova, Marcela
PY  - 2021
TI  - The molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in the Czech Republic
JF  - Journal of Antimicrobial Chemotherapy
VL  - 76
IS  - 1
SP  - 55-64
EP  - 55-64
PB  - Oxford University Press
SN  - 03057453
KW  - molecular epidemiology
KW  - methicillin-resistant Staphylococcus aureus
UR  - https://academic.oup.com/jac/article-abstract/76/1/55/5942696?redirectedFrom=fulltext
N2  - Objectives: To gain data on the current molecular epidemiology and resistance of MRSA in the Czech Republic. Methods: Between September 2017 and January 2018, a total of 441 single-patient MRSA isolates were collected from 11 Czech hospitals and analysed by spa typing, SCCmec typing, antibiotic susceptibility testing, detection of the PVL toxin and the arcA gene. Results: Of all MRSA isolates, 81.41% (n = 359) belonged to the CC5-MRSA clone represented by the spa types t003 (n = 136), t586 (n = 92), t014 (n = 81), t002 (n = 20) and other spa types (n = 30); a majority of the CC5 isolates (n = 348, 96.94%) carried SCCmec type II. The occurrence of CC5-MRSA was more Likely in older inpatients and associated with a healthcare origin (P< 0.001). The CC5-MRSA isolates were resistant to more antimicrobial drugs compared with the other MRSAs (P< 0.001). Interestingly, t586 was detected in blood samples more often than the other spa types and, contrary to other spa types belonging to CC5-MRSA, t586 was not associated with patients of advanced age. Other frequently found Lineages were CC8 (n = 17), CC398 (n = 11) and CC59 (n = 10). The presence of the PVL was detected in 8.62% (n = 38) of the MRSA isolates. Conclusions: The healthcare-associated CC5-MRSA-II Lineage (t003, t586, t014) was found to be predominant in the Czech Republic. t586 is a newly emerging spa type in the Czech Republic, yet reported rarely in other countries. Our observations stress the need for MRSA surveillance in the Czech Republic in order to monitor changes in MRSA epidemiology.
ER  -

TKADLEC, Jan, Vaclav CAPEK, Marie BRAJEROVA, Eva SMELIKOVA, Oto MELTER, Tamara BERGEROVA, Sylvia POLIVKOVA, Magda BALEJOVA, Marketa HANSLIANOVA, Daniela FACKOVA, Katerina NERADOVA, Renata TEJKALOVÁ, Iva VAGNEROVA, Natasa BARTONIKOVA, Eva CHMELAROVA, Pavel DREVINEK and Marcela KRUTOVA. The molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in the Czech Republic. \textit{Journal of Antimicrobial Chemotherapy}. OXFORD: Oxford University Press, 2021, vol.~76, No~1, p.~55-64. ISSN~0305-7453. Available from: https://dx.doi.org/10.1093/jac/dkaa404.

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