Timing of spermatogonial stem cell transplantation affects the
spermatogenic recovery outcome in mice

J 2021

Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice

MEDRANO, J. V., Ivana AĆIMOVIĆ, A. NAVARRO-GOMEZLECHON, I. NOGUERA, A. PELLICER et. al.

Basic information

Original name

Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice

Authors

MEDRANO, J. V. (guarantor), Ivana AĆIMOVIĆ (688 Serbia, belonging to the institution), A. NAVARRO-GOMEZLECHON, I. NOGUERA, A. PELLICER, M. M. ANDRES and E. NOVELLA-MAESTRE

Edition

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, NEW YORK, SPRINGER, 2021, 1071-2690

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.723

RIV identification code

RIV/00216224:14110/21:00121703

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s11626-020-00531-9

UT WoS

000606180100002

Keywords in English

Fertility preservation; Spermatogonial stem cell transplantation; Spermatogenic recovery; Gonadotoxic onset; Timing

Abstract

V originále

Spermatogonial stem cell transplantation (SSCT) is a strategy that has demonstrated to be feasible to restore spermatogenesis in animal models when it is performed shortly after the gonadotoxic onset to destroy their endogenous germ cells. However, in the case of boys subjected to fertility preservation, future transplantations will be performed with a delay of many years. In order to study how timing of SSCT affects donor-derived spermatogenic recovery in mice, we compared the percentage of spermatogenic tubule cross-sections within testes of 59 C57BL/6NCrl mice distributed in 6 groups: group 1, untreated mice controls (n = 9); group 2, mice that received a single dose of busulfan 40 mg/kg (n = 10); group 3, mice that received two additional doses of busulfan 10 mg/kg every 5 weeks (n = 10); group 4 (SSCT-A), mice subjected to a standard SSCT performed 5 weeks after a single injection of busulfan 40 mg/kg (n = 10); group 5 (SSCT-B), mice subjected to a delayed SSCT performed 15 weeks after a single injection of busulfan 40 mg/kg (n = 10); and group 6 (SSCT-C), mice subjected to a delayed SSCT with two additional doses of busulfan 10 mg/kg every 5 weeks (n = 10). Spermatogenic recovery in standard SSCT-A and SSCT-C groups ranged between 22.29 and 22.65%, compared with a lower recovery rate of 11.54% showed in the SSCT-B group. However, donor contribution resulted higher in standard SSCT-A, representing a 69.71% of cross-sections, compared with the rest of conditions ranging from 34.69 to 35.42%. Overall, we concluded that a delay in the SSCT from the gonadotoxic onset decreases the efficiency of donor-derived spermatogenic recovery in mice.

Citovat

MEDRANO, J. V., Ivana AĆIMOVIĆ, A. NAVARRO-GOMEZLECHON, I. NOGUERA, A. PELLICER, M. M. ANDRES and E. NOVELLA-MAESTRE. Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL. NEW YORK: SPRINGER, 2021, vol. 57, No 1, p. 21-29. ISSN 1071-2690. Available from: https://dx.doi.org/10.1007/s11626-020-00531-9.

@article{1773328,
   author = {Medrano, J. V. and Aćimović, Ivana and NavarroandGomezlechon, A. and Noguera, I. and Pellicer, A. and Andres, M. M. and NovellaandMaestre, E.},
   article_location = {NEW YORK},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s11626-020-00531-9},
   keywords = {Fertility preservation; Spermatogonial stem cell transplantation; Spermatogenic recovery; Gonadotoxic onset; Timing},
   language = {eng},
   issn = {1071-2690},
   journal = {IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL},
   title = {Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice},
   url = {https://link.springer.com/article/10.1007%2Fs11626-020-00531-9},
   volume = {57},
   year = {2021}
}

TY  - JOUR
ID  - 1773328
AU  - Medrano, J. V. - Aćimović, Ivana - Navarro-Gomezlechon, A. - Noguera, I. - Pellicer, A. - Andres, M. M. - Novella-Maestre, E.
PY  - 2021
TI  - Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice
JF  - IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
VL  - 57
IS  - 1
SP  - 21-29
EP  - 21-29
PB  - SPRINGER
SN  - 10712690
KW  - Fertility preservation
KW  - Spermatogonial stem cell transplantation
KW  - Spermatogenic recovery
KW  - Gonadotoxic onset
KW  - Timing
UR  - https://link.springer.com/article/10.1007%2Fs11626-020-00531-9
N2  - Spermatogonial stem cell transplantation (SSCT) is a strategy that has demonstrated to be feasible to restore spermatogenesis in animal models when it is performed shortly after the gonadotoxic onset to destroy their endogenous germ cells. However, in the case of boys subjected to fertility preservation, future transplantations will be performed with a delay of many years. In order to study how timing of SSCT affects donor-derived spermatogenic recovery in mice, we compared the percentage of spermatogenic tubule cross-sections within testes of 59 C57BL/6NCrl mice distributed in 6 groups: group 1, untreated mice controls (n = 9); group 2, mice that received a single dose of busulfan 40 mg/kg (n = 10); group 3, mice that received two additional doses of busulfan 10 mg/kg every 5 weeks (n = 10); group 4 (SSCT-A), mice subjected to a standard SSCT performed 5 weeks after a single injection of busulfan 40 mg/kg (n = 10); group 5 (SSCT-B), mice subjected to a delayed SSCT performed 15 weeks after a single injection of busulfan 40 mg/kg (n = 10); and group 6 (SSCT-C), mice subjected to a delayed SSCT with two additional doses of busulfan 10 mg/kg every 5 weeks (n = 10). Spermatogenic recovery in standard SSCT-A and SSCT-C groups ranged between 22.29 and 22.65%, compared with a lower recovery rate of 11.54% showed in the SSCT-B group. However, donor contribution resulted higher in standard SSCT-A, representing a 69.71% of cross-sections, compared with the rest of conditions ranging from 34.69 to 35.42%. Overall, we concluded that a delay in the SSCT from the gonadotoxic onset decreases the efficiency of donor-derived spermatogenic recovery in mice.
ER  -

MEDRANO, J. V., Ivana A$\backslash$'CIMOVI$\backslash$'C, A. NAVARRO-GOMEZLECHON, I. NOGUERA, A. PELLICER, M. M. ANDRES and E. NOVELLA-MAESTRE. Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice. \textit{IN VITRO CELLULAR \&{} DEVELOPMENTAL BIOLOGY-ANIMAL}. NEW YORK: SPRINGER, 2021, vol.~57, No~1, p.~21-29. ISSN~1071-2690. Available from: https://dx.doi.org/10.1007/s11626-020-00531-9.

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