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@article{1773328,
author = {Medrano, J. V. and Aćimović, Ivana and NavarroandGomezlechon, A. and Noguera, I. and Pellicer, A. and Andres, M. M. and NovellaandMaestre, E.},
article_location = {NEW YORK},
article_number = {1},
doi = {http://dx.doi.org/10.1007/s11626-020-00531-9},
keywords = {Fertility preservation; Spermatogonial stem cell transplantation; Spermatogenic recovery; Gonadotoxic onset; Timing},
language = {eng},
issn = {1071-2690},
journal = {IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL},
title = {Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice},
url = {https://link.springer.com/article/10.1007%2Fs11626-020-00531-9},
volume = {57},
year = {2021}
}
TY - JOUR
ID - 1773328
AU - Medrano, J. V. - Aćimović, Ivana - Navarro-Gomezlechon, A. - Noguera, I. - Pellicer, A. - Andres, M. M. - Novella-Maestre, E.
PY - 2021
TI - Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice
JF - IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
VL - 57
IS - 1
SP - 21-29
EP - 21-29
PB - SPRINGER
SN - 10712690
KW - Fertility preservation
KW - Spermatogonial stem cell transplantation
KW - Spermatogenic recovery
KW - Gonadotoxic onset
KW - Timing
UR - https://link.springer.com/article/10.1007%2Fs11626-020-00531-9
N2 - Spermatogonial stem cell transplantation (SSCT) is a strategy that has demonstrated to be feasible to restore spermatogenesis in animal models when it is performed shortly after the gonadotoxic onset to destroy their endogenous germ cells. However, in the case of boys subjected to fertility preservation, future transplantations will be performed with a delay of many years. In order to study how timing of SSCT affects donor-derived spermatogenic recovery in mice, we compared the percentage of spermatogenic tubule cross-sections within testes of 59 C57BL/6NCrl mice distributed in 6 groups: group 1, untreated mice controls (n = 9); group 2, mice that received a single dose of busulfan 40 mg/kg (n = 10); group 3, mice that received two additional doses of busulfan 10 mg/kg every 5 weeks (n = 10); group 4 (SSCT-A), mice subjected to a standard SSCT performed 5 weeks after a single injection of busulfan 40 mg/kg (n = 10); group 5 (SSCT-B), mice subjected to a delayed SSCT performed 15 weeks after a single injection of busulfan 40 mg/kg (n = 10); and group 6 (SSCT-C), mice subjected to a delayed SSCT with two additional doses of busulfan 10 mg/kg every 5 weeks (n = 10). Spermatogenic recovery in standard SSCT-A and SSCT-C groups ranged between 22.29 and 22.65%, compared with a lower recovery rate of 11.54% showed in the SSCT-B group. However, donor contribution resulted higher in standard SSCT-A, representing a 69.71% of cross-sections, compared with the rest of conditions ranging from 34.69 to 35.42%. Overall, we concluded that a delay in the SSCT from the gonadotoxic onset decreases the efficiency of donor-derived spermatogenic recovery in mice.
ER -
MEDRANO, J. V., Ivana A$\backslash$'CIMOVI$\backslash$'C, A. NAVARRO-GOMEZLECHON, I. NOGUERA, A. PELLICER, M. M. ANDRES and E. NOVELLA-MAESTRE. Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice. \textit{IN VITRO CELLULAR \&{} DEVELOPMENTAL BIOLOGY-ANIMAL}. NEW YORK: SPRINGER, 2021, vol.~57, No~1, p.~21-29. ISSN~1071-2690. Available from: https://dx.doi.org/10.1007/s11626-020-00531-9.
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