Cervical Gardnerella vaginalis in women with preterm prelabor
rupture of membranes

J 2021

Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes

KACEROVSKY, Marian, Lenka PLISKOVA, Radka BOLEHOVSKA, Daniel LESKO, Romana GERYCHOVÁ et. al.

Basic information

Original name

Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes

Authors

KACEROVSKY, Marian (guarantor), Lenka PLISKOVA (203 Czech Republic), Radka BOLEHOVSKA (203 Czech Republic), Daniel LESKO (203 Czech Republic), Romana GERYCHOVÁ (203 Czech Republic, belonging to the institution), Petr JANKŮ (203 Czech Republic, belonging to the institution), Petr MATLAK (203 Czech Republic), Ondrej SIMETKA (203 Czech Republic), Jaroslav STRANIK (203 Czech Republic), Tomas FAIST (203 Czech Republic), Jan MLS (203 Czech Republic), Peter VESCICIK (203 Czech Republic), Bo JACOBSSON and Ivana MUSILOVA (203 Czech Republic)

Edition

Plos one, San Francisco, Public Library of Science, 2021, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.752

RIV identification code

RIV/00216224:14110/21:00121733

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pone.0245937

UT WoS

000612929300038

Keywords in English

Gardnerella vaginalis; preterm prelabor rupture of membranes

Abstract

V originále

Objective To determine the association between microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) and the cervical prevalence of Gardnerella vaginalis DNA in pregnancies with preterm prelabor rupture of membrane (PPROM). Method In total, 405 women with singleton pregnancies complicated with PPROM were included. Cervical fluid and amniotic fluid samples were collected at the time of admission. Bacterial and G. vaginalis DNA were assessed in the cervical fluid samples using quantitative PCR technique. Concentrations of interleukin-6 and MIAC were evaluated in the amniotic fluid samples. Loads of G. vaginalis DNA. 1% of the total cervical bacterial DNA were used to define the cervical prevalence of G. vaginalis as abundant. Based on the MIAC and IAI, women were categorized into four groups: with intra- amniotic infection (both MIAC and IAI), with sterile IAI (IAI without MIAC), with MIAC without IAI, and without either MIAC or IAI. Results The presence of the abundant cervical G. vaginalis was related to MIAC (with: 65% vs. without: 44%; p = 0.0004) but not IAI (with: 52% vs. without: 48%; p = 0.70). Women with MIAC without IAI had the highest load of the cervical G. vaginalis DNA (median 2.0 x 10(4) copies DNA/mL) and the highest presence of abundant cervical G. vaginalis (73%). Conclusions In women with PPROM, the presence of cervical G. vaginalis was associated with MIAC, mainly without the concurrent presence of IAI.

Citovat

KACEROVSKY, Marian, Lenka PLISKOVA, Radka BOLEHOVSKA, Daniel LESKO, Romana GERYCHOVÁ, Petr JANKŮ, Petr MATLAK, Ondrej SIMETKA, Jaroslav STRANIK, Tomas FAIST, Jan MLS, Peter VESCICIK, Bo JACOBSSON and Ivana MUSILOVA. Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes. Plos one. San Francisco: Public Library of Science, 2021, vol. 16, No 1, p. 1-19. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0245937.

@article{1774699,
   author = {Kacerovsky, Marian and Pliskova, Lenka and Bolehovska, Radka and Lesko, Daniel and Gerychová, Romana and Janků, Petr and Matlak, Petr and Simetka, Ondrej and Stranik, Jaroslav and Faist, Tomas and Mls, Jan and Vescicik, Peter and Jacobsson, Bo and Musilova, Ivana},
   article_location = {San Francisco},
   article_number = {1},
   doi = {http://dx.doi.org/10.1371/journal.pone.0245937},
   keywords = {Gardnerella vaginalis; preterm prelabor rupture of membranes},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes},
   url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245937},
   volume = {16},
   year = {2021}
}

TY  - JOUR
ID  - 1774699
AU  - Kacerovsky, Marian - Pliskova, Lenka - Bolehovska, Radka - Lesko, Daniel - Gerychová, Romana - Janků, Petr - Matlak, Petr - Simetka, Ondrej - Stranik, Jaroslav - Faist, Tomas - Mls, Jan - Vescicik, Peter - Jacobsson, Bo - Musilova, Ivana
PY  - 2021
TI  - Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes
JF  - Plos one
VL  - 16
IS  - 1
SP  - 1-19
EP  - 1-19
PB  - Public Library of Science
SN  - 19326203
KW  - Gardnerella vaginalis
KW  - preterm prelabor rupture of membranes
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245937
N2  - Objective To determine the association between microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) and the cervical prevalence of Gardnerella vaginalis DNA in pregnancies with preterm prelabor rupture of membrane (PPROM). Method In total, 405 women with singleton pregnancies complicated with PPROM were included. Cervical fluid and amniotic fluid samples were collected at the time of admission. Bacterial and G. vaginalis DNA were assessed in the cervical fluid samples using quantitative PCR technique. Concentrations of interleukin-6 and MIAC were evaluated in the amniotic fluid samples. Loads of G. vaginalis DNA. 1% of the total cervical bacterial DNA were used to define the cervical prevalence of G. vaginalis as abundant. Based on the MIAC and IAI, women were categorized into four groups: with intra- amniotic infection (both MIAC and IAI), with sterile IAI (IAI without MIAC), with MIAC without IAI, and without either MIAC or IAI. Results The presence of the abundant cervical G. vaginalis was related to MIAC (with: 65% vs. without: 44%; p = 0.0004) but not IAI (with: 52% vs. without: 48%; p = 0.70). Women with MIAC without IAI had the highest load of the cervical G. vaginalis DNA (median 2.0 x 10(4) copies DNA/mL) and the highest presence of abundant cervical G. vaginalis (73%). Conclusions In women with PPROM, the presence of cervical G. vaginalis was associated with MIAC, mainly without the concurrent presence of IAI.
ER  -

KACEROVSKY, Marian, Lenka PLISKOVA, Radka BOLEHOVSKA, Daniel LESKO, Romana GERYCHOVÁ, Petr JANKŮ, Petr MATLAK, Ondrej SIMETKA, Jaroslav STRANIK, Tomas FAIST, Jan MLS, Peter VESCICIK, Bo JACOBSSON and Ivana MUSILOVA. Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes. \textit{Plos one}. San Francisco: Public Library of Science, 2021, vol.~16, No~1, p.~1-19. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0245937.

Detailed Information on Publication Record