Dynamic miRNA changes during the process of epileptogenesis in
an infantile and adult-onset model

J 2021

Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model

BENCÚROVÁ, Petra, Jiří BALOUN, Jakub HYNŠT, Jan OPPELT, Hana KUBOVÁ et. al.

Basic information

Original name

Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model

Authors

BENCÚROVÁ, Petra (703 Slovakia, belonging to the institution), Jiří BALOUN (203 Czech Republic, belonging to the institution), Jakub HYNŠT (203 Czech Republic, belonging to the institution), Jan OPPELT (203 Czech Republic, belonging to the institution), Hana KUBOVÁ (203 Czech Republic), Šárka POSPÍŠILOVÁ (203 Czech Republic, guarantor, belonging to the institution) and Milan BRÁZDIL (203 Czech Republic, belonging to the institution)

Edition

Nature Scientific Reports, London, NATURE RESEARCH, 2021, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10700 1.7 Other natural sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.996

RIV identification code

RIV/00216224:14110/21:00119034

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-021-89084-9

UT WoS

000656986200006

Keywords in English

Dynamic miRNA changes; epileptogenesis; infantile and adult-onset model

Abstract

V originále

Temporal lobe epilepsy (TLE) is the most common epilepsy type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to the early onset of the disease. Although the miRNA involvement in TLE is widely studied, the relationship between the onset-age and miRNA expression has not been addressed. Here, we investigated the miRNA profile of infantile and adult-onset TLE in rats combining sequencing and PCR. Since miRNA expression changes with the disease progression, we scrutinized miRNA dynamics across three stages: acute, latent, and chronic. We report that infantile-onset TLE leads to changes in the expression of fewer miRNAs across these stages. Interestingly, the miRNA profile in the acute stage of infantile-onset TLE overlaps in dysregulation of miR-132-5p, -205, and -211-3p with the chronic stage of the disease starting in adulthood. The analysis of putative targets linked the majority of dysregulated miRNAs with pathways involved in epilepsy. Our profiling uncovered miRNA expression characteristic for infantile and adulthood-onset epileptogenesis, suggesting the distinct biology underlying TLE in the onset age-dependent matter. Our results indicate the necessity of addressing the onset age as an important parameter in future epilepsy research.

Links

GA16-04726S, research and development project

Name: miRNA a biochemické dráhy v epilepsii

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1595/2020, interní kód MU

Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VIII (Acronym: VýDiTeHeMa VIII)

Investor: Masaryk University

90091, large research infrastructures

Name: NCMG

Citovat

BENCÚROVÁ, Petra, Jiří BALOUN, Jakub HYNŠT, Jan OPPELT, Hana KUBOVÁ, Šárka POSPÍŠILOVÁ and Milan BRÁZDIL. Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model. Nature Scientific Reports. London: NATURE RESEARCH, 2021, vol. 11, No 1, p. 1-14. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-89084-9.

@article{1779390,
   author = {Bencúrová, Petra and Baloun, Jiří and Hynšt, Jakub and Oppelt, Jan and Kubová, Hana and Pospíšilová, Šárka and Brázdil, Milan},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-021-89084-9},
   keywords = {Dynamic miRNA changes; epileptogenesis; infantile and adult-onset model},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model},
   url = {https://www.nature.com/articles/s41598-021-89084-9.pdf},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1779390
AU  - Bencúrová, Petra - Baloun, Jiří - Hynšt, Jakub - Oppelt, Jan - Kubová, Hana - Pospíšilová, Šárka - Brázdil, Milan
PY  - 2021
TI  - Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model
JF  - Nature Scientific Reports
VL  - 11
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Dynamic miRNA changes
KW  - epileptogenesis
KW  - infantile and adult-onset model
UR  - https://www.nature.com/articles/s41598-021-89084-9.pdf
N2  - Temporal lobe epilepsy (TLE) is the most common epilepsy type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to the early onset of the disease. Although the miRNA involvement in TLE is widely studied, the relationship between the onset-age and miRNA expression has not been addressed. Here, we investigated the miRNA profile of infantile and adult-onset TLE in rats combining sequencing and PCR. Since miRNA expression changes with the disease progression, we scrutinized miRNA dynamics across three stages: acute, latent, and chronic. We report that infantile-onset TLE leads to changes in the expression of fewer miRNAs across these stages. Interestingly, the miRNA profile in the acute stage of infantile-onset TLE overlaps in dysregulation of miR-132-5p, -205, and -211-3p with the chronic stage of the disease starting in adulthood. The analysis of putative targets linked the majority of dysregulated miRNAs with pathways involved in epilepsy. Our profiling uncovered miRNA expression characteristic for infantile and adulthood-onset epileptogenesis, suggesting the distinct biology underlying TLE in the onset age-dependent matter. Our results indicate the necessity of addressing the onset age as an important parameter in future epilepsy research.
ER  -

BENCÚROVÁ, Petra, Jiří BALOUN, Jakub HYNŠT, Jan OPPELT, Hana KUBOVÁ, Šárka POSPÍŠILOVÁ and Milan BRÁZDIL. Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2021, vol.~11, No~1, p.~1-14. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-89084-9.

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