A Robust Mammary Organoid System to Model Lactation and
Involution-like Processes

J 2021

A Robust Mammary Organoid System to Model Lactation and Involution-like Processes

CHARIFOU, Elsa, Jakub SUMBAL, Zuzana KOLEDOVÁ, Han LI, Aurélie CHICHE et. al.

Basic information

Original name

A Robust Mammary Organoid System to Model Lactation and Involution-like Processes

Authors

CHARIFOU, Elsa, Jakub SUMBAL (203 Czech Republic, belonging to the institution), Zuzana KOLEDOVÁ (703 Slovakia), Han LI and Aurélie CHICHE (guarantor)

Edition

Bio-protocol, Sunnyvale, Bio-protocol, 2021, 2331-8325

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10602 Biology , Evolutionary biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14110/21:00121913

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.21769/BioProtoc.3996

UT WoS

000642293100012

Keywords in English

Mouse; Mammary gland; 3D organoid; Ex vivo; Lactation; Involution

Abstract

V originále

The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding how dysregulation can lead to breast cancer initiation and progression. Three-dimensional (3D) mammary organoids offer many exciting possibilities for the study of tissue development and breast cancer. In the present protocol derived from Sumbal et al., we describe a straightforward 3D organoid system for the study of lactation and involution ex vivo. We use primary and passaged mouse mammary organoids stimulated with fibroblast growth factor 2 (FGF2) and prolactin to model the three cycles of mouse mammary gland lactation and involution processes. This 3D organoid model represents a valuable tool to study late postnatal mammary gland development and breast cancer, in particular postpartum-associated breast cancer.

Links

MUNI/A/1565/2018, interní kód MU

Name: Zdroje pro tkáňové inženýrství 9 (Acronym: TissueEng 9)

Investor: Masaryk University, Category A

MUNI/A/1689/2020, interní kód MU

Name: Zdroje pro tkáňové inženýrství 11 (Acronym: TissueEng 11)

Investor: Masaryk University

MUNI/G/1446/2018, interní kód MU

Name: Deciphering the mechanisms of mammary epithelial branched pattern formation through iterative biological and mathematical modelling

Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects

ROZV/28/LF19/2020, interní kód MU

Name: Regulace morfogeneze epitelu mléčné žlázy pomocí mechanických sil a dynamiky signalizace

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

CHARIFOU, Elsa, Jakub SUMBAL, Zuzana KOLEDOVÁ, Han LI and Aurélie CHICHE. A Robust Mammary Organoid System to Model Lactation and Involution-like Processes. Bio-protocol. Sunnyvale: Bio-protocol, 2021, vol. 11, No 8, p. "e3996", 19 pp. ISSN 2331-8325. Available from: https://dx.doi.org/10.21769/BioProtoc.3996.

@article{1781777,
   author = {Charifou, Elsa and Sumbal, Jakub and Koledová, Zuzana and Li, Han and Chiche, Aurélie},
   article_location = {Sunnyvale},
   article_number = {8},
   doi = {http://dx.doi.org/10.21769/BioProtoc.3996},
   keywords = {Mouse; Mammary gland; 3D organoid; Ex vivo; Lactation; Involution},
   language = {eng},
   issn = {2331-8325},
   journal = {Bio-protocol},
   title = {A Robust Mammary Organoid System to Model Lactation and Involution-like Processes},
   url = {https://bio-protocol.org/e3996},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1781777
AU  - Charifou, Elsa - Sumbal, Jakub - Koledová, Zuzana - Li, Han - Chiche, Aurélie
PY  - 2021
TI  - A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
JF  - Bio-protocol
VL  - 11
IS  - 8
SP  - "e3996"
EP  - "e3996"
PB  - Bio-protocol
SN  - 23318325
KW  - Mouse
KW  - Mammary gland
KW  - 3D organoid
KW  - Ex vivo
KW  - Lactation
KW  - Involution
UR  - https://bio-protocol.org/e3996
N2  - The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding how dysregulation can lead to breast cancer initiation and progression. Three-dimensional (3D) mammary organoids offer many exciting possibilities for the study of tissue development and breast cancer. In the present protocol derived from Sumbal et al., we describe a straightforward 3D organoid system for the study of lactation and involution ex vivo. We use primary and passaged mouse mammary organoids stimulated with fibroblast growth factor 2 (FGF2) and prolactin to model the three cycles of mouse mammary gland lactation and involution processes. This 3D organoid model represents a valuable tool to study late postnatal mammary gland development and breast cancer, in particular postpartum-associated breast cancer.
ER  -

CHARIFOU, Elsa, Jakub SUMBAL, Zuzana KOLEDOVÁ, Han LI and Aurélie CHICHE. A Robust Mammary Organoid System to Model Lactation and Involution-like Processes. \textit{Bio-protocol}. Sunnyvale: Bio-protocol, 2021, vol.~11, No~8, p.~''e3996'', 19 pp. ISSN~2331-8325. Available from: https://dx.doi.org/10.21769/BioProtoc.3996.

Detailed Information on Publication Record