Digital PCR can provide improved BCR-ABL1 detection in chronic
myeloid leukemia patients in deep molecular response and
sensitivity of standard quantitative methods using EAC assays

J 2021

Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays

SMITALOVÁ, Dagmar, D. DVORAKOVA, Zdeněk RÁČIL and Marianna ROMŽOVÁ

Basic information

Original name

Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays

Authors

SMITALOVÁ, Dagmar (203 Czech Republic, belonging to the institution), D. DVORAKOVA, Zdeněk RÁČIL (203 Czech Republic) and Marianna ROMŽOVÁ (703 Slovakia, belonging to the institution)

Edition

Practical Laboratory Medicine, AMSTERDAM, ELSEVIER, 2021, 2352-5517

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14740/21:00120108

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.plabm.2021.e00210

UT WoS

000653682600011

Keywords in English

Chronic myeloid leukemia; BCR-ABL1 monitoring; GeneXpert BCR-ABL Monitor assay; RT-qPCR; Digital PCR

Abstract

V originále

BCR-ABL1 molecular detection using quantitative PCR (qPCR) methods is the golden standard of chronic myeloid leukemia (CML) monitoring. However, due to variable sensitivity of qPCR assays across laboratories, alternative methods are tested. Digital PCR (dPCR) has been suggested as a robust and reproducible option. Here we present a comparison of droplet dPCR with routinely used reverse-transcription qPCR (RT-qPCR) and automated GeneXpert systems. Detection limit of dPCR was above 3 BCR-ABL1 copies, although due to background amplification the resulting sensitivity was 0.01% BCR-ABL1 (MR4.0). Nevertheless, in comparison with GeneXpert, dPCR categorized more than 50% of the patients into different MR groups, showing a potential for improved BCR-ABL1 detection.

Links

MUNI/A/1105/2018, interní kód MU

Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VI (Acronym: VýDiTeHeMA VI)

Investor: Masaryk University, Category A

MUNI/A/1395/2019, interní kód MU

Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VII (Acronym: VýDiTeHeMa VII)

Investor: Masaryk University, Category A

NV17-30397A, research and development project

Name: Mutační analýza primitivních buněčných populací u chronické myeloidní leukémie: za hranicí BCR-ABL1

Citovat

SMITALOVÁ, Dagmar, D. DVORAKOVA, Zdeněk RÁČIL and Marianna ROMŽOVÁ. Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays. Practical Laboratory Medicine. AMSTERDAM: ELSEVIER, 2021, vol. 25, MAY, p. nestrankovano, 6 pp. ISSN 2352-5517. Available from: https://dx.doi.org/10.1016/j.plabm.2021.e00210.

@article{1782161,
   author = {Smitalová, Dagmar and Dvorakova, D. and Ráčil, Zdeněk and Romžová, Marianna},
   article_location = {AMSTERDAM},
   article_number = {MAY},
   doi = {http://dx.doi.org/10.1016/j.plabm.2021.e00210},
   keywords = {Chronic myeloid leukemia; BCR-ABL1 monitoring; GeneXpert BCR-ABL Monitor assay; RT-qPCR; Digital PCR},
   language = {eng},
   issn = {2352-5517},
   journal = {Practical Laboratory Medicine},
   title = {Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays},
   url = {https://www.sciencedirect.com/science/article/pii/S235255172100010X?via%3Dihub},
   volume = {25},
   year = {2021}
}

TY  - JOUR
ID  - 1782161
AU  - Smitalová, Dagmar - Dvorakova, D. - Ráčil, Zdeněk - Romžová, Marianna
PY  - 2021
TI  - Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays
JF  - Practical Laboratory Medicine
VL  - 25
IS  - MAY
SP  - nestrankovano
EP  - nestrankovano
PB  - ELSEVIER
SN  - 23525517
KW  - Chronic myeloid leukemia
KW  - BCR-ABL1 monitoring
KW  - GeneXpert BCR-ABL Monitor assay
KW  - RT-qPCR
KW  - Digital PCR
UR  - https://www.sciencedirect.com/science/article/pii/S235255172100010X?via%3Dihub
N2  - BCR-ABL1 molecular detection using quantitative PCR (qPCR) methods is the golden standard of chronic myeloid leukemia (CML) monitoring. However, due to variable sensitivity of qPCR assays across laboratories, alternative methods are tested. Digital PCR (dPCR) has been suggested as a robust and reproducible option. Here we present a comparison of droplet dPCR with routinely used reverse-transcription qPCR (RT-qPCR) and automated GeneXpert systems. Detection limit of dPCR was above 3 BCR-ABL1 copies, although due to background amplification the resulting sensitivity was 0.01% BCR-ABL1 (MR4.0). Nevertheless, in comparison with GeneXpert, dPCR categorized more than 50% of the patients into different MR groups, showing a potential for improved BCR-ABL1 detection.
ER  -

SMITALOVÁ, Dagmar, D. DVORAKOVA, Zdeněk RÁČIL and Marianna ROMŽOVÁ. Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays. \textit{Practical Laboratory Medicine}. AMSTERDAM: ELSEVIER, 2021, vol.~25, MAY, p.~nestrankovano, 6 pp. ISSN~2352-5517. Available from: https://dx.doi.org/10.1016/j.plabm.2021.e00210.

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