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@article{1782961,
author = {Kripnerova, M. and Parmar, H.S. and Šána, Jiří and Kopková, Alena and Radová, Lenka and Sopper, S. and Biernacki, K. and Jedlicka, J. and Kohoutova, M. and Kuncova, J. and Peychl, J. and Rudolf, E. and Cervinka, M. and Houdek, Z. and Dvorak, P. and Houfkova, K. and Pesta, M. and Tuma, Z. and Dolejsova, M. and Tichanek, F. and Babuska, V. and Leba, M. and Slabý, Ondřej and Hatina, J.},
article_location = {Basel},
article_number = {11},
doi = {http://dx.doi.org/10.3390/jcm10112297},
keywords = {fibrosarcoma; progression series; invasiveness; transcriptome; Ccl8},
language = {eng},
issn = {2077-0383},
journal = {Journal of Clinical Medicine},
title = {Complex Interplay of Genes Underlies Invasiveness in Fibrosarcoma Progression Model},
url = {https://www.mdpi.com/2077-0383/10/11/2297},
volume = {10},
year = {2021}
}
TY - JOUR
ID - 1782961
AU - Kripnerova, M. - Parmar, H.S. - Šána, Jiří - Kopková, Alena - Radová, Lenka - Sopper, S. - Biernacki, K. - Jedlicka, J. - Kohoutova, M. - Kuncova, J. - Peychl, J. - Rudolf, E. - Cervinka, M. - Houdek, Z. - Dvorak, P. - Houfkova, K. - Pesta, M. - Tuma, Z. - Dolejsova, M. - Tichanek, F. - Babuska, V. - Leba, M. - Slabý, Ondřej - Hatina, J.
PY - 2021
TI - Complex Interplay of Genes Underlies Invasiveness in Fibrosarcoma Progression Model
JF - Journal of Clinical Medicine
VL - 10
IS - 11
SP - 2297
EP - 2297
PB - MDPI
SN - 20770383
KW - fibrosarcoma
KW - progression series
KW - invasiveness
KW - transcriptome
KW - Ccl8
UR - https://www.mdpi.com/2077-0383/10/11/2297
N2 - Sarcomas are a heterogeneous group of mesenchymal tumours, with a great variability in their clinical behaviour. While our knowledge of sarcoma initiation has advanced rapidly in recent years, relatively little is known about mechanisms of sarcoma progression. JUN-murine fibrosarcoma progression series consists of four sarcoma cell lines, JUN-1, JUN-2, JUN-2fos-3, and JUN-3. JUN-1 and -2 were established from a single tumour initiated in a H2K/v-jun transgenic mouse, JUN-3 originates from a different tumour in the same animal, and JUN-2fos-3 results from a targeted in vitro transformation of the JUN-2 cell line. The JUN-1, -2, and -3 cell lines represent a linear progression from the least transformed JUN-2 to the most transformed JUN-3, with regard to all the transformation characteristics studied, while the JUN-2fos-3 cell line exhibits a unique transformation mode, with little deregulation of cell growth and proliferation, but pronounced motility and invasiveness. The invasive sarcoma sublines JUN-2fos-3 and JUN-3 show complex metabolic profiles, with activation of both mitochondrial oxidative phosphorylation and glycolysis and a significant increase in spared respiratory capacity. The specific transcriptomic profile of invasive sublines features very complex biological relationships across the identified genes and proteins, with accentuated autocrine control of motility and angiogenesis. Pharmacologic inhibition of one of the autocrine motility factors identified, Ccl8, significantly diminished both motility and invasiveness of the highly transformed fibrosarcoma cell. This progression series could be greatly valuable for deciphering crucial aspects of sarcoma progression and defining new prognostic markers and potential therapeutic targets.
ER -
KRIPNEROVA, M., H.S. PARMAR, Jiří ŠÁNA, Alena KOPKOVÁ, Lenka RADOVÁ, S. SOPPER, K. BIERNACKI, J. JEDLICKA, M. KOHOUTOVA, J. KUNCOVA, J. PEYCHL, E. RUDOLF, M. CERVINKA, Z. HOUDEK, P. DVORAK, K. HOUFKOVA, M. PESTA, Z. TUMA, M. DOLEJSOVA, F. TICHANEK, V. BABUSKA, M. LEBA, Ondřej SLABÝ and J. HATINA. Complex Interplay of Genes Underlies Invasiveness in Fibrosarcoma Progression Model. \textit{Journal of Clinical Medicine}. Basel: MDPI, 2021, vol.~10, No~11, p.~2297-2324. ISSN~2077-0383. Available from: https://dx.doi.org/10.3390/jcm10112297.
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