Healing and Angiogenic Properties of Collagen/Chitosan
Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In
Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

J 2021

Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

VOJTOVA, Lucy, Veronika PAVLINAKOVA, Johana MUCHOVA, Katarina KACVINSKA, Jana BRTNIKOVA et. al.

Basic information

Original name

Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

Authors

VOJTOVA, Lucy (203 Czech Republic), Veronika PAVLINAKOVA (203 Czech Republic, guarantor), Johana MUCHOVA (203 Czech Republic), Katarina KACVINSKA, Jana BRTNIKOVA (203 Czech Republic), Martin KNOZ (203 Czech Republic, belonging to the institution), Břetislav LIPOVÝ (203 Czech Republic, belonging to the institution), Martin FALDYNA (203 Czech Republic), Eduard GOPFERT (203 Czech Republic), Jakub HOLOUBEK (203 Czech Republic, belonging to the institution), Zdeněk PAVLOVSKÝ (203 Czech Republic, belonging to the institution), Monika VICENOVA (203 Czech Republic), Veronika HEFKA BLAHNOVA (203 Czech Republic), Vanessa HEARNDEN and Eva FILOVA (203 Czech Republic)

Edition

BIOMEDICINES, BASEL, MDPI, 2021, 2227-9059

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.757

RIV identification code

RIV/00216224:14110/21:00121944

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/biomedicines9060590

UT WoS

000665273200001

Keywords in English

collagen; chitosan; scaffold; FGF2; skin regeneration; tissue engineering

Abstract

V originále

Wound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 degrees C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB(R) were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB(R) already at very low concentrations (0.01 mu g/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB(R) protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.

Citovat

VOJTOVA, Lucy, Veronika PAVLINAKOVA, Johana MUCHOVA, Katarina KACVINSKA, Jana BRTNIKOVA, Martin KNOZ, Břetislav LIPOVÝ, Martin FALDYNA, Eduard GOPFERT, Jakub HOLOUBEK, Zdeněk PAVLOVSKÝ, Monika VICENOVA, Veronika HEFKA BLAHNOVA, Vanessa HEARNDEN and Eva FILOVA. Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation. BIOMEDICINES. BASEL: MDPI, 2021, vol. 9, No 6, p. 1-27. ISSN 2227-9059. Available from: https://dx.doi.org/10.3390/biomedicines9060590.

@article{1782963,
   author = {Vojtova, Lucy and Pavlinakova, Veronika and Muchova, Johana and Kacvinska, Katarina and Brtnikova, Jana and Knoz, Martin and Lipový, Břetislav and Faldyna, Martin and Gopfert, Eduard and Holoubek, Jakub and Pavlovský, Zdeněk and Vicenova, Monika and Hefka Blahnova, Veronika and Hearnden, Vanessa and Filova, Eva},
   article_location = {BASEL},
   article_number = {6},
   doi = {http://dx.doi.org/10.3390/biomedicines9060590},
   keywords = {collagen; chitosan; scaffold; FGF2; skin regeneration; tissue engineering},
   language = {eng},
   issn = {2227-9059},
   journal = {BIOMEDICINES},
   title = {Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation},
   url = {https://www.mdpi.com/2227-9059/9/6/590},
   volume = {9},
   year = {2021}
}

TY  - JOUR
ID  - 1782963
AU  - Vojtova, Lucy - Pavlinakova, Veronika - Muchova, Johana - Kacvinska, Katarina - Brtnikova, Jana - Knoz, Martin - Lipový, Břetislav - Faldyna, Martin - Gopfert, Eduard - Holoubek, Jakub - Pavlovský, Zdeněk - Vicenova, Monika - Hefka Blahnova, Veronika - Hearnden, Vanessa - Filova, Eva
PY  - 2021
TI  - Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation
JF  - BIOMEDICINES
VL  - 9
IS  - 6
SP  - 1-27
EP  - 1-27
PB  - MDPI
SN  - 22279059
KW  - collagen
KW  - chitosan
KW  - scaffold
KW  - FGF2
KW  - skin regeneration
KW  - tissue engineering
UR  - https://www.mdpi.com/2227-9059/9/6/590
N2  - Wound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 degrees C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB(R) were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB(R) already at very low concentrations (0.01 mu g/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB(R) protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
ER  -

VOJTOVA, Lucy, Veronika PAVLINAKOVA, Johana MUCHOVA, Katarina KACVINSKA, Jana BRTNIKOVA, Martin KNOZ, Břetislav LIPOVÝ, Martin FALDYNA, Eduard GOPFERT, Jakub HOLOUBEK, Zdeněk PAVLOVSKÝ, Monika VICENOVA, Veronika HEFKA BLAHNOVA, Vanessa HEARNDEN and Eva FILOVA. Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation. \textit{BIOMEDICINES}. BASEL: MDPI, 2021, vol.~9, No~6, p.~1-27. ISSN~2227-9059. Available from: https://dx.doi.org/10.3390/biomedicines9060590.

Detailed Information on Publication Record