| VOJTOVA, Lucy, Veronika PAVLINAKOVA, Johana MUCHOVA, Katarina KACVINSKA, Jana BRTNIKOVA, Martin KNOZ, Břetislav LIPOVÝ, Martin FALDYNA, Eduard GOPFERT, Jakub HOLOUBEK, Zdeněk PAVLOVSKÝ, Monika VICENOVA, Veronika HEFKA BLAHNOVA, Vanessa HEARNDEN and Eva FILOVA. Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation. BIOMEDICINES. BASEL: MDPI, 2021, vol. 9, No 6, p. 1-27. ISSN 2227-9059. Available from: https://dx.doi.org/10.3390/biomedicines9060590. |
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@article{1782963,
author = {Vojtova, Lucy and Pavlinakova, Veronika and Muchova, Johana and Kacvinska, Katarina and Brtnikova, Jana and Knoz, Martin and Lipový, Břetislav and Faldyna, Martin and Gopfert, Eduard and Holoubek, Jakub and Pavlovský, Zdeněk and Vicenova, Monika and Hefka Blahnova, Veronika and Hearnden, Vanessa and Filova, Eva},
article_location = {BASEL},
article_number = {6},
doi = {http://dx.doi.org/10.3390/biomedicines9060590},
keywords = {collagen; chitosan; scaffold; FGF2; skin regeneration; tissue engineering},
language = {eng},
issn = {2227-9059},
journal = {BIOMEDICINES},
title = {Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation},
url = {https://www.mdpi.com/2227-9059/9/6/590},
volume = {9},
year = {2021}
}
TY - JOUR
ID - 1782963
AU - Vojtova, Lucy - Pavlinakova, Veronika - Muchova, Johana - Kacvinska, Katarina - Brtnikova, Jana - Knoz, Martin - Lipový, Břetislav - Faldyna, Martin - Gopfert, Eduard - Holoubek, Jakub - Pavlovský, Zdeněk - Vicenova, Monika - Hefka Blahnova, Veronika - Hearnden, Vanessa - Filova, Eva
PY - 2021
TI - Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation
JF - BIOMEDICINES
VL - 9
IS - 6
SP - 1-27
EP - 1-27
PB - MDPI
SN - 22279059
KW - collagen
KW - chitosan
KW - scaffold
KW - FGF2
KW - skin regeneration
KW - tissue engineering
UR - https://www.mdpi.com/2227-9059/9/6/590
N2 - Wound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 degrees C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB(R) were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB(R) already at very low concentrations (0.01 mu g/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB(R) protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
ER -
VOJTOVA, Lucy, Veronika PAVLINAKOVA, Johana MUCHOVA, Katarina KACVINSKA, Jana BRTNIKOVA, Martin KNOZ, Břetislav LIPOVÝ, Martin FALDYNA, Eduard GOPFERT, Jakub HOLOUBEK, Zdeněk PAVLOVSKÝ, Monika VICENOVA, Veronika HEFKA BLAHNOVA, Vanessa HEARNDEN and Eva FILOVA. Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation. \textit{BIOMEDICINES}. BASEL: MDPI, 2021, vol.~9, No~6, p.~1-27. ISSN~2227-9059. Available from: https://dx.doi.org/10.3390/biomedicines9060590.
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