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@article{1782980,
author = {Ronzhina, Marina and Stračina, Tibor and Lacinova, Lubica and Ondacova, Katarina and Pavlovicova, Michaela and Marsanova, Lucie and Smisek, Radovan and Janousek, Oto and Fialová, Kateřina and Kolarova, Jana and Nováková, Marie and Provaznik, Ivo},
article_location = {Lausanne},
article_number = {June},
doi = {http://dx.doi.org/10.3389/fphys.2021.667065},
keywords = {di-4-ANEPPS; rabbit isolated heart; myocardial ischemia; electrogram analysis; patch-clamp; voltage-sensitive dye},
language = {eng},
issn = {1664-042X},
journal = {Frontiers in Physiology},
title = {Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart},
url = {https://www.frontiersin.org/articles/10.3389/fphys.2021.667065/full},
volume = {12},
year = {2021}
}
TY - JOUR
ID - 1782980
AU - Ronzhina, Marina - Stračina, Tibor - Lacinova, Lubica - Ondacova, Katarina - Pavlovicova, Michaela - Marsanova, Lucie - Smisek, Radovan - Janousek, Oto - Fialová, Kateřina - Kolarova, Jana - Nováková, Marie - Provaznik, Ivo
PY - 2021
TI - Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart
JF - Frontiers in Physiology
VL - 12
IS - June
SP - 1-15
EP - 1-15
PB - Frontiers Media
SN - 1664042X
KW - di-4-ANEPPS
KW - rabbit isolated heart
KW - myocardial ischemia
KW - electrogram analysis
KW - patch-clamp
KW - voltage-sensitive dye
UR - https://www.frontiersin.org/articles/10.3389/fphys.2021.667065/full
N2 - Aims Although voltage-sensitive dye di-4-ANEPPS is a common tool for mapping cardiac electrical activity, reported effects on electrophysiological parameters are rather. The main goals of the study were to reveal effects of the dye on rabbit isolated heart and to verify, whether rabbit isolated heart stained with di-4-ANEPPS is a suitable tool for myocardial ischemia investigation. Methods and Results Study involved experiments on stained (n = 9) and non-stained (n = 11) Langendorff perfused rabbit isolated hearts. Electrophysiological effects of the dye were evaluated by analysis of various electrogram (EG) parameters using common paired and unpaired statistical tests. It was shown that staining the hearts with di-4-ANEPPS leads to only short-term sporadic prolongation of impulse conduction through atria and atrioventricular node. On the other hand, significant irreversible slowing of heart rate and ventricular conduction were found in stained hearts as compared to controls. In patch clamp experiments, significant inhibition of sodium current density was observed in differentiated NG108-15 cells stained by the dye. Although no significant differences in mean number of ventricular premature beats were found between the stained and the non-stained hearts in ischemia as well as in reperfusion, all abovementioned results indicate increased arrhythmogenicity. In isolated hearts during ischemia, prominent ischemic patterns appeared in the stained hearts with 3-4 min delay as compared to the non-stained ones. Moreover, the ischemic changes did not achieve the same magnitude as in controls even after 10 min of ischemia. It resulted in poor performance of ischemia detection by proposed EG parameters, as was quantified by receiver operating characteristics analysis. Conclusion Our results demonstrate significant direct irreversible effect of di-4-ANEPPS on spontaneous heart rate and ventricular impulse conduction in rabbit isolated heart model. Particularly, this should be considered when di-4-ANEPPS is used in ischemia studies in rabbit. Delayed attenuated response of such hearts to ischemia might lead to misinterpretation of obtained results.
ER -
RONZHINA, Marina, Tibor STRAČINA, Lubica LACINOVA, Katarina ONDACOVA, Michaela PAVLOVICOVA, Lucie MARSANOVA, Radovan SMISEK, Oto JANOUSEK, Kateřina FIALOVÁ, Jana KOLAROVA, Marie NOVÁKOVÁ and Ivo PROVAZNIK. Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart. \textit{Frontiers in Physiology}. Lausanne: Frontiers Media, 2021, vol.~12, June, p.~1-15. ISSN~1664-042X. Available from: https://dx.doi.org/10.3389/fphys.2021.667065.
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