Distinct Responses of Arabidopsis Telomeres and Transposable
Elements to Zebularine Exposure

J 2021

Distinct Responses of Arabidopsis Telomeres and Transposable Elements to Zebularine Exposure

KONEČNÁ, Klára, Pavla SOVÁKOVÁ, Karin ANTEKOVÁ, Jiří FAJKUS, Miloslava FOJTOVÁ et. al.

Basic information

Original name

Distinct Responses of Arabidopsis Telomeres and Transposable Elements to Zebularine Exposure

Authors

KONEČNÁ, Klára (203 Czech Republic, belonging to the institution), Pavla SOVÁKOVÁ (203 Czech Republic, belonging to the institution), Karin ANTEKOVÁ (703 Slovakia, belonging to the institution), Jiří FAJKUS (203 Czech Republic, belonging to the institution) and Miloslava FOJTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.208

RIV identification code

RIV/00216224:14740/21:00122005

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/ijms22010468

UT WoS

000606186200001

Keywords in English

Arabidopsis thaliana; epigenetics; cytosine methylation; telomeres; transposable elements; zebularine

Abstract

V originále

Involvement of epigenetic mechanisms in the regulation of telomeres and transposable elements (TEs), genomic regions with the protective and potentially detrimental function, respectively, has been frequently studied. Here, we analyzed telomere lengths in Arabidopsis thaliana plants of Columbia, Landsberg erecta and Wassilevskija ecotypes exposed repeatedly to the hypomethylation drug zebularine during germination. Shorter telomeres were detected in plants growing from seedlings germinated in the presence of zebularine with a progression in telomeric phenotype across generations, relatively high inter-individual variability, and diverse responses among ecotypes. Interestingly, the extent of telomere shortening in zebularine Columbia and Wassilevskija plants corresponded to the transcriptional activation of TEs, suggesting a correlated response of these genomic elements to the zebularine treatment. Changes in lengths of telomeres and levels of TE transcripts in leaves were not always correlated with a hypomethylation of cytosines located in these regions, indicating a cytosine methylation-independent level of their regulation. These observations, including differences among ecotypes together with distinct dynamics of the reversal of the disruption of telomere homeostasis and TEs transcriptional activation, reflect a complex involvement of epigenetic processes in the regulation of crucial genomic regions. Our results further demonstrate the ability of plant cells to cope with these changes without a critical loss of the genome stability.

Links

EF16_026/0008446, research and development project

Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

LTC20003, research and development project

Name: Dynamics of telomeres and rDNA loci in plant cell nuclei

Investor: Ministry of Education, Youth and Sports of the CR, INTER-COST

Citovat

KONEČNÁ, Klára, Pavla SOVÁKOVÁ, Karin ANTEKOVÁ, Jiří FAJKUS and Miloslava FOJTOVÁ. Distinct Responses of Arabidopsis Telomeres and Transposable Elements to Zebularine Exposure. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2021, vol. 22, No 1, p. "468", 14 pp. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms22010468.

@article{1784121,
   author = {Konečná, Klára and Sováková, Pavla and Anteková, Karin and Fajkus, Jiří and Fojtová, Miloslava},
   article_location = {Basel},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/ijms22010468},
   keywords = {Arabidopsis thaliana; epigenetics; cytosine methylation; telomeres; transposable elements; zebularine},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Distinct Responses of Arabidopsis Telomeres and Transposable Elements to Zebularine Exposure},
   url = {https://www.mdpi.com/1422-0067/22/1/468},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1784121
AU  - Konečná, Klára - Sováková, Pavla - Anteková, Karin - Fajkus, Jiří - Fojtová, Miloslava
PY  - 2021
TI  - Distinct Responses of Arabidopsis Telomeres and Transposable Elements to Zebularine Exposure
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 1
SP  - "468"
EP  - "468"
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - Arabidopsis thaliana
KW  - epigenetics
KW  - cytosine methylation
KW  - telomeres
KW  - transposable elements
KW  - zebularine
UR  - https://www.mdpi.com/1422-0067/22/1/468
N2  - Involvement of epigenetic mechanisms in the regulation of telomeres and transposable elements (TEs), genomic regions with the protective and potentially detrimental function, respectively, has been frequently studied. Here, we analyzed telomere lengths in Arabidopsis thaliana plants of Columbia, Landsberg erecta and Wassilevskija ecotypes exposed repeatedly to the hypomethylation drug zebularine during germination. Shorter telomeres were detected in plants growing from seedlings germinated in the presence of zebularine with a progression in telomeric phenotype across generations, relatively high inter-individual variability, and diverse responses among ecotypes. Interestingly, the extent of telomere shortening in zebularine Columbia and Wassilevskija plants corresponded to the transcriptional activation of TEs, suggesting a correlated response of these genomic elements to the zebularine treatment. Changes in lengths of telomeres and levels of TE transcripts in leaves were not always correlated with a hypomethylation of cytosines located in these regions, indicating a cytosine methylation-independent level of their regulation. These observations, including differences among ecotypes together with distinct dynamics of the reversal of the disruption of telomere homeostasis and TEs transcriptional activation, reflect a complex involvement of epigenetic processes in the regulation of crucial genomic regions. Our results further demonstrate the ability of plant cells to cope with these changes without a critical loss of the genome stability.
ER  -

KONEČNÁ, Klára, Pavla SOVÁKOVÁ, Karin ANTEKOVÁ, Jiří FAJKUS and Miloslava FOJTOVÁ. Distinct Responses of Arabidopsis Telomeres and Transposable Elements to Zebularine Exposure. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2021, vol.~22, No~1, p.~''468'', 14 pp. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms22010468.

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