Extraordinary diversity of telomeres, telomerase RNAs and their
template regions in Saccharomycetaceae

J 2021

Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae

PEŠKA, Vratislav, Petr FAJKUS, Michal BUBENÍK, Václav BRÁZDA, Natália BOHÁLOVÁ et. al.

Basic information

Original name

Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae

Authors

PEŠKA, Vratislav (203 Czech Republic, guarantor), Petr FAJKUS (203 Czech Republic, belonging to the institution), Michal BUBENÍK (203 Czech Republic, belonging to the institution), Václav BRÁZDA (203 Czech Republic), Natália BOHÁLOVÁ (703 Slovakia, belonging to the institution), Vojtěch DVOŘÁČEK (203 Czech Republic), Jiří FAJKUS (203 Czech Republic, belonging to the institution) and Sonia GARCIA (724 Spain)

Edition

Scientific Reports, Nature Research, 2021, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.996

RIV identification code

RIV/00216224:14740/21:00119109

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/s41598-021-92126-x

UT WoS

000664915500010

Keywords in English

DNA END-REPLICATION; SECONDARY STRUCTURE; G-QUADRUPLEXES SEQUENCES; REPEATS IDENTIFICATION; EVOLUTION; GENOME

Abstract

V originále

Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically close groups. TRs of several Saccharomycetaceae were recently identified, however, many of these remained uncharacterised in the template region. Here we show that this is mainly due to high variability in telomere sequence. We predicted the telomere sequences using Tandem Repeats Finder and then we identified corresponding putative template regions in TR candidates. Remarkably long telomere units and the corresponding putative TRs were found in Tetrapisispora species. Notably, variable lengths of the annealing sequence of the template region (1-10 nt) were found. Consequently, species with the same telomere sequence may not harbour identical TR templates. Thus, TR sequence alone can be used to predict a template region and telomere sequence, but not to determine these exactly. A conserved feature of telomere sequences, tracts of adjacent Gs, led us to test the propensity of individual telomere sequences to form G4. The results show highly diverse values of G4-propensity, indicating the lack of ubiquitous conservation of this feature across Saccharomycetaceae.

Links

GX20-01331X, research and development project

Name: Biogeneze a evoluce telomerázy u rostlin

Investor: Czech Science Foundation

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PEŠKA, Vratislav, Petr FAJKUS, Michal BUBENÍK, Václav BRÁZDA, Natália BOHÁLOVÁ, Vojtěch DVOŘÁČEK, Jiří FAJKUS and Sonia GARCIA. Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae. Scientific Reports. Nature Research, 2021, vol. 11, No 1, p. "12784", 11 pp. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-92126-x.

@article{1784130,
   author = {Peška, Vratislav and Fajkus, Petr and Bubeník, Michal and Brázda, Václav and Bohálová, Natália and Dvořáček, Vojtěch and Fajkus, Jiří and Garcia, Sonia},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-021-92126-x},
   keywords = {DNA END-REPLICATION; SECONDARY STRUCTURE; G-QUADRUPLEXES SEQUENCES; REPEATS IDENTIFICATION; EVOLUTION; GENOME},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae},
   url = {https://www.nature.com/articles/s41598-021-92126-x},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1784130
AU  - Peška, Vratislav - Fajkus, Petr - Bubeník, Michal - Brázda, Václav - Bohálová, Natália - Dvořáček, Vojtěch - Fajkus, Jiří - Garcia, Sonia
PY  - 2021
TI  - Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
JF  - Scientific Reports
VL  - 11
IS  - 1
SP  - "12784"
EP  - "12784"
PB  - Nature Research
SN  - 20452322
KW  - DNA END-REPLICATION
KW  - SECONDARY STRUCTURE
KW  - G-QUADRUPLEXES SEQUENCES
KW  - REPEATS IDENTIFICATION
KW  - EVOLUTION
KW  - GENOME
UR  - https://www.nature.com/articles/s41598-021-92126-x
N2  - Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically close groups. TRs of several Saccharomycetaceae were recently identified, however, many of these remained uncharacterised in the template region. Here we show that this is mainly due to high variability in telomere sequence. We predicted the telomere sequences using Tandem Repeats Finder and then we identified corresponding putative template regions in TR candidates. Remarkably long telomere units and the corresponding putative TRs were found in Tetrapisispora species. Notably, variable lengths of the annealing sequence of the template region (1-10 nt) were found. Consequently, species with the same telomere sequence may not harbour identical TR templates. Thus, TR sequence alone can be used to predict a template region and telomere sequence, but not to determine these exactly. A conserved feature of telomere sequences, tracts of adjacent Gs, led us to test the propensity of individual telomere sequences to form G4. The results show highly diverse values of G4-propensity, indicating the lack of ubiquitous conservation of this feature across Saccharomycetaceae.
ER  -

PEŠKA, Vratislav, Petr FAJKUS, Michal BUBENÍK, Václav BRÁZDA, Natália BOHÁLOVÁ, Vojtěch DVOŘÁČEK, Jiří FAJKUS and Sonia GARCIA. Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae. \textit{Scientific Reports}. Nature Research, 2021, vol.~11, No~1, p.~''12784'', 11 pp. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-92126-x.

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