J 2021

Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin

HUA, Qing, Ondřej ADAMOVSKÝ, Hana VESPALCOVÁ, Jonna BOYDA, Jordan T. SCHMIDT et. al.

Basic information

Original name

Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin

Authors

HUA, Qing (156 China), Ondřej ADAMOVSKÝ (203 Czech Republic, guarantor, belonging to the institution), Hana VESPALCOVÁ (203 Czech Republic, belonging to the institution), Jonna BOYDA (840 United States of America), Jordan T. SCHMIDT (840 United States of America), Marianne KOZUCH (840 United States of America), Serena L. M. CRAFT (840 United States of America), Pamela E. GINN (840 United States of America), Stanislav SMATANA (703 Slovakia, belonging to the institution), Eva BUDINSKÁ (703 Slovakia, belonging to the institution), Maria PERSICO (380 Italy, belonging to the institution), Joseph H., Jr. BISESI (840 United States of America) and Christopher J. MARTYNIUK (840 United States of America)

Edition

Environmental Pollution, Elsevier Ltd. 2021, 0269-7491

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 9.988

RIV identification code

RIV/00216224:14310/21:00122022

Organization unit

Faculty of Science

UT WoS

000600560400034

Keywords in English

Microbiome; Pesticide; Aquatic; Ecotoxicology; Pathology

Tags

Tags

International impact, Reviewed
Změněno: 1/11/2021 15:55, Mgr. Marie Šípková, DiS.

Abstract

V originále

Dietary exposure to chemicals alters the diversity of microbiome communities and can lead to pathophysiological changes in the gastrointestinal system. The organochlorine pesticide dieldrin is a persistent environmental contaminant that bioaccumulates in fatty tissue of aquatic organisms. The objectives of this study were to determine whether environmentally-relevant doses of dieldrin altered gastrointestinal morphology and the microbiome of zebrafish. Adult zebrafish at similar to 4 months of age were fed a measured amount of feed containing either a solvent control or one of two doses of dieldrin (measured at 16, and 163.5 ng/g dry weight) for 4 months. Dieldrin body burden levels in zebrafish after four-month exposure were 0 (control), 11.47 +/- 1.13 ng/g (low dose) and 18.32 +/- 1.32 ng/g (high dose) wet weight [mean +/- std]. Extensive histopathology at the whole organism level revealed that dieldrin exposure did not induce notable tissue pathology, including the gastrointestinal tract. A repeated measure mixed model analysis revealed that, while fish gained weight over time, there were no dieldrin-specific effects on body weight. Fecal content was collected from the gastrointestinal tract of males and 16S rRNA gene sequencing conducted. Dieldrin at a measured feed dose of 16 ng/g reduced the abundance of Firmicutes, a phylum involved in energy resorption. At the level of class, there was a decrease in abundance of Clostridia and Betaproteobacteria, and an increase in Verrucomicrobiae species. We used a computational approach called predicted relative metabolomic turnover (PRMT) to predict how a shift in microbial community composition affects exchange of metabolites. Dieldrinwas predicted to affect metabolic turnover of uroporphyrinogen I and coproporphyrinogen I [enzyme]-cysteine, hydrogen selenide, selenite, and methyl-selenic acid in the fish gastrointestinal system. These pathways are related to bacterial heme biosynthesis and selenium metabolism. Our study demonstrates that dietary exposures to dieldrin can alter microbiota composition over 4 months, however the long-term consequences of such impacts are not well understood.

Links

EF17_043/0009632, research and development project
Name: CETOCOEN Excellence
LM2018121, research and development project
Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI
857560, interní kód MU
(CEP code: EF17_043/0009632)
Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)
Investor: European Union, Spreading excellence and widening participation