Detailed Information on Publication Record
2021
Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin
HUA, Qing, Ondřej ADAMOVSKÝ, Hana VESPALCOVÁ, Jonna BOYDA, Jordan T. SCHMIDT et. al.Basic information
Original name
Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin
Authors
HUA, Qing (156 China), Ondřej ADAMOVSKÝ (203 Czech Republic, guarantor, belonging to the institution), Hana VESPALCOVÁ (203 Czech Republic, belonging to the institution), Jonna BOYDA (840 United States of America), Jordan T. SCHMIDT (840 United States of America), Marianne KOZUCH (840 United States of America), Serena L. M. CRAFT (840 United States of America), Pamela E. GINN (840 United States of America), Stanislav SMATANA (703 Slovakia, belonging to the institution), Eva BUDINSKÁ (703 Slovakia, belonging to the institution), Maria PERSICO (380 Italy, belonging to the institution), Joseph H., Jr. BISESI (840 United States of America) and Christopher J. MARTYNIUK (840 United States of America)
Edition
Environmental Pollution, Elsevier Ltd. 2021, 0269-7491
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10511 Environmental sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 9.988
RIV identification code
RIV/00216224:14310/21:00122022
Organization unit
Faculty of Science
UT WoS
000600560400034
Keywords in English
Microbiome; Pesticide; Aquatic; Ecotoxicology; Pathology
Tags
Tags
International impact, Reviewed
Změněno: 1/11/2021 15:55, Mgr. Marie Šípková, DiS.
Abstract
V originále
Dietary exposure to chemicals alters the diversity of microbiome communities and can lead to pathophysiological changes in the gastrointestinal system. The organochlorine pesticide dieldrin is a persistent environmental contaminant that bioaccumulates in fatty tissue of aquatic organisms. The objectives of this study were to determine whether environmentally-relevant doses of dieldrin altered gastrointestinal morphology and the microbiome of zebrafish. Adult zebrafish at similar to 4 months of age were fed a measured amount of feed containing either a solvent control or one of two doses of dieldrin (measured at 16, and 163.5 ng/g dry weight) for 4 months. Dieldrin body burden levels in zebrafish after four-month exposure were 0 (control), 11.47 +/- 1.13 ng/g (low dose) and 18.32 +/- 1.32 ng/g (high dose) wet weight [mean +/- std]. Extensive histopathology at the whole organism level revealed that dieldrin exposure did not induce notable tissue pathology, including the gastrointestinal tract. A repeated measure mixed model analysis revealed that, while fish gained weight over time, there were no dieldrin-specific effects on body weight. Fecal content was collected from the gastrointestinal tract of males and 16S rRNA gene sequencing conducted. Dieldrin at a measured feed dose of 16 ng/g reduced the abundance of Firmicutes, a phylum involved in energy resorption. At the level of class, there was a decrease in abundance of Clostridia and Betaproteobacteria, and an increase in Verrucomicrobiae species. We used a computational approach called predicted relative metabolomic turnover (PRMT) to predict how a shift in microbial community composition affects exchange of metabolites. Dieldrinwas predicted to affect metabolic turnover of uroporphyrinogen I and coproporphyrinogen I [enzyme]-cysteine, hydrogen selenide, selenite, and methyl-selenic acid in the fish gastrointestinal system. These pathways are related to bacterial heme biosynthesis and selenium metabolism. Our study demonstrates that dietary exposures to dieldrin can alter microbiota composition over 4 months, however the long-term consequences of such impacts are not well understood.
Links
EF17_043/0009632, research and development project |
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LM2018121, research and development project |
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857560, interní kód MU (CEP code: EF17_043/0009632) |
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