Simultaneous quantitative profiling of clinically relevant
immune markers in neonatal stool swabs to reveal inflammation

J 2021

Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation

VIDOVÁ, Veronika, Eliška BENEŠOVÁ, Jana KLÁNOVÁ, Vojtěch THON, Zdeněk SPÁČIL et. al.

Basic information

Original name

Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation

Authors

VIDOVÁ, Veronika (203 Czech Republic, belonging to the institution), Eliška BENEŠOVÁ (203 Czech Republic, belonging to the institution), Jana KLÁNOVÁ (203 Czech Republic, belonging to the institution), Vojtěch THON (203 Czech Republic, belonging to the institution) and Zdeněk SPÁČIL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nature Scientific Reports, London, NATURE RESEARCH, 2021, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10700 1.7 Other natural sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.996

RIV identification code

RIV/00216224:14310/21:00119119

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-021-89384-0

UT WoS

000656946100012

Keywords in English

EOSINOPHIL-DERIVED NEUROTOXIN; INTESTINAL INFLAMMATION; FOOD ALLERGY; CESAREAN-SECTION; GRANULE PROTEINS; BOWEL DISEASES; EARLY-LIFE; MECONIUM; GUT; ALPHA-1-ANTITRYPSIN

Abstract

V originále

An aberrant immune response developed early in life may trigger inflammatory bowel disease (IBD) and food allergies (e.g., celiac disease). Fecal levels of immune markers categorize an inflammatory response (e.g., food allergy, autoimmune) paralleled with the initial microbial colonization. The immunoaffinity assays are routinely applied to quantify circulating immune protein markers in blood/serum. However, a reliable, multiplex assay to quantify fecal levels of immune proteins is unavailable. We developed mass spectrometry assays to simultaneously quantify fecal calprotectin, myeloperoxidase, eosinophil-derived neurotoxin, eosinophil cationic protein, alpha-1-antitrypsin 1, and adaptive immunity effectors in 134 neonatal stool swabs. We optimized extraction and proteolytic protocol and validated the multiplex assay in terms of linearity of response (>100; typically 0.04 to 14.77 mu g/mg of total protein), coefficient of determination (R-2;>0.99), the limit of detection (LOD; 0.003 to 0.04 mu g/mg of total protein), the limit of quantification (LOQ; 0.009 to 0.122 mu g/mg of total protein) and robustness. The median CV of intra- and interday precision was 9.8% and 14.1%, respectively. We quantified breast milk-derived IGHA2 to differentiate meconium from feces samples and to detect the first food intake. An early life profiling of immune markers reflects disrupted intestinal homeostasis, and it is perhaps suitable for pre-symptomatic interception of IBD and food allergies.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

GJ17-24592Y, research and development project

Name: Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou

Investor: Czech Science Foundation

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

Citovat

VIDOVÁ, Veronika, Eliška BENEŠOVÁ, Jana KLÁNOVÁ, Vojtěch THON and Zdeněk SPÁČIL. Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation. Nature Scientific Reports. London: NATURE RESEARCH, 2021, vol. 11, No 1, p. 1-9. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-89384-0.

@article{1785039,
   author = {Vidová, Veronika and Benešová, Eliška and Klánová, Jana and Thon, Vojtěch and Spáčil, Zdeněk},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-021-89384-0},
   keywords = {EOSINOPHIL-DERIVED NEUROTOXIN; INTESTINAL INFLAMMATION; FOOD ALLERGY; CESAREAN-SECTION; GRANULE PROTEINS; BOWEL DISEASES; EARLY-LIFE; MECONIUM; GUT; ALPHA-1-ANTITRYPSIN},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation},
   url = {https://www.nature.com/articles/s41598-021-89384-0},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1785039
AU  - Vidová, Veronika - Benešová, Eliška - Klánová, Jana - Thon, Vojtěch - Spáčil, Zdeněk
PY  - 2021
TI  - Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation
JF  - Nature Scientific Reports
VL  - 11
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - NATURE RESEARCH
SN  - 20452322
KW  - EOSINOPHIL-DERIVED NEUROTOXIN
KW  - INTESTINAL INFLAMMATION
KW  - FOOD ALLERGY
KW  - CESAREAN-SECTION
KW  - GRANULE PROTEINS
KW  - BOWEL DISEASES
KW  - EARLY-LIFE
KW  - MECONIUM
KW  - GUT
KW  - ALPHA-1-ANTITRYPSIN
UR  - https://www.nature.com/articles/s41598-021-89384-0
N2  - An aberrant immune response developed early in life may trigger inflammatory bowel disease (IBD) and food allergies (e.g., celiac disease). Fecal levels of immune markers categorize an inflammatory response (e.g., food allergy, autoimmune) paralleled with the initial microbial colonization. The immunoaffinity assays are routinely applied to quantify circulating immune protein markers in blood/serum. However, a reliable, multiplex assay to quantify fecal levels of immune proteins is unavailable. We developed mass spectrometry assays to simultaneously quantify fecal calprotectin, myeloperoxidase, eosinophil-derived neurotoxin, eosinophil cationic protein, alpha-1-antitrypsin 1, and adaptive immunity effectors in 134 neonatal stool swabs. We optimized extraction and proteolytic protocol and validated the multiplex assay in terms of linearity of response (>100; typically 0.04 to 14.77 mu g/mg of total protein), coefficient of determination (R-2;>0.99), the limit of detection (LOD; 0.003 to 0.04 mu g/mg of total protein), the limit of quantification (LOQ; 0.009 to 0.122 mu g/mg of total protein) and robustness. The median CV of intra- and interday precision was 9.8% and 14.1%, respectively. We quantified breast milk-derived IGHA2 to differentiate meconium from feces samples and to detect the first food intake. An early life profiling of immune markers reflects disrupted intestinal homeostasis, and it is perhaps suitable for pre-symptomatic interception of IBD and food allergies.
ER  -

VIDOVÁ, Veronika, Eliška BENEŠOVÁ, Jana KLÁNOVÁ, Vojtěch THON and Zdeněk SPÁČIL. Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2021, vol.~11, No~1, p.~1-9. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-89384-0.

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