Streptomyces poriferorum sp. nov., a novel marine
sponge-derived Actinobacteria species expressing anti-MRSA
activity

J 2021

Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity

SANDOVA-POWERS, Megan, Stanislava KRÁLOVÁ, Giang-Son NGUYEN, Dorelle V. FAWWAL, Kristin DEGNES et. al.

Basic information

Original name

Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity

Authors

SANDOVA-POWERS, Megan, Stanislava KRÁLOVÁ (703 Slovakia, belonging to the institution), Giang-Son NGUYEN, Dorelle V. FAWWAL, Kristin DEGNES, Anna Sofia LEWIN, Geir KLINKENBERG, Alexander WENTZEL and Mark R. LILES (guarantor)

Edition

Systematic and Applied Microbiology, Elsevier GmbH, 2021, 0723-2020

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.064

RIV identification code

RIV/00216224:14310/21:00122057

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.syapm.2021.126244

UT WoS

000697699400004

Keywords in English

Streptomyces poriferorum sp. nov.; marine sponge; Actinobacteria; polyphasic taxonomy; Trondheim fjord

Abstract

V originále

Marine sponges represent a rich source of uncharacterized microbial diversity, and many are host to microorganisms that produce biologically active specialized metabolites. Here, a polyphasic approach was used to characterize two Actinobacteria strains, P01-B04T and P01-F02, that were isolated from the marine sponges Geodia barretti (Bowerbank, 1858) and Antho dichotoma (Esper, 1794), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains P01-B04T and P01-F02 are closely related to Streptomyces beijiangensis DSM 41794T, Streptomyces laculatispora NRRL B-24909T, and Streptomyces brevispora NRRL B-24910T. The two strains showed nearly identical 16S rRNA gene sequences (99.93%), and the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) relatedness values were 99.96% and 99.6%, respectively, suggesting that these strains are affiliated with the same species. Chemotaxonomic and culture characteristics of both strains were also consistent with the genus Streptomyces, while phenotypic properties, genome-based comparisons, and phylogenomic analyses distinguished strains P01-B04T and P01-F02 from their closest phylogenetic relatives. In silico analysis predicted that the 8.9 Mb genome of P01-B04T contains at least 41 biosynthetic gene clusters (BGCs) encoding secondary metabolites, indicating that this strain could express diverse bioactive metabolites; in support of this prediction, this strain expressed antibacterial activity against Gram-positive bacteria including a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA) EAMC30. Based on these results, the marine sponge-associated isolates represent a novel species of the genus Streptomyces, for which the name Streptomyces poriferorum sp. nov. is proposed, with P01-B04T (= DSM 111306T = CCM 9048T) as the type strain.

Citovat

SANDOVA-POWERS, Megan, Stanislava KRÁLOVÁ, Giang-Son NGUYEN, Dorelle V. FAWWAL, Kristin DEGNES, Anna Sofia LEWIN, Geir KLINKENBERG, Alexander WENTZEL and Mark R. LILES. Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity. Systematic and Applied Microbiology. Elsevier GmbH, 2021, vol. 44, No 5, p. 126244-126256. ISSN 0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2021.126244.

@article{1785540,
   author = {SandovaandPowers, Megan and Králová, Stanislava and Nguyen, GiangandSon and Fawwal, Dorelle V. and Degnes, Kristin and Lewin, Anna Sofia and Klinkenberg, Geir and Wentzel, Alexander and Liles, Mark R.},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.syapm.2021.126244},
   keywords = {Streptomyces poriferorum sp. nov.; marine sponge; Actinobacteria; polyphasic taxonomy; Trondheim fjord},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity},
   url = {https://doi.org/10.1016/j.syapm.2021.126244},
   volume = {44},
   year = {2021}
}

TY  - JOUR
ID  - 1785540
AU  - Sandova-Powers, Megan - Králová, Stanislava - Nguyen, Giang-Son - Fawwal, Dorelle V. - Degnes, Kristin - Lewin, Anna Sofia - Klinkenberg, Geir - Wentzel, Alexander - Liles, Mark R.
PY  - 2021
TI  - Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity
JF  - Systematic and Applied Microbiology
VL  - 44
IS  - 5
SP  - 126244
EP  - 126244
PB  - Elsevier GmbH
SN  - 07232020
KW  - Streptomyces poriferorum sp. nov.
KW  - marine sponge
KW  - Actinobacteria
KW  - polyphasic taxonomy
KW  - Trondheim fjord
UR  - https://doi.org/10.1016/j.syapm.2021.126244
N2  - Marine sponges represent a rich source of uncharacterized microbial diversity, and many are host to microorganisms that produce biologically active specialized metabolites. Here, a polyphasic approach was used to characterize two Actinobacteria strains, P01-B04T and P01-F02, that were isolated from the marine sponges Geodia barretti (Bowerbank, 1858) and Antho dichotoma (Esper, 1794), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains P01-B04T and P01-F02 are closely related to Streptomyces beijiangensis DSM 41794T, Streptomyces laculatispora NRRL B-24909T, and Streptomyces brevispora NRRL B-24910T. The two strains showed nearly identical 16S rRNA gene sequences (99.93%), and the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) relatedness values were 99.96% and 99.6%, respectively, suggesting that these strains are affiliated with the same species. Chemotaxonomic and culture characteristics of both strains were also consistent with the genus Streptomyces, while phenotypic properties, genome-based comparisons, and phylogenomic analyses distinguished strains P01-B04T and P01-F02 from their closest phylogenetic relatives. In silico analysis predicted that the 8.9 Mb genome of P01-B04T contains at least 41 biosynthetic gene clusters (BGCs) encoding secondary metabolites, indicating that this strain could express diverse bioactive metabolites; in support of this prediction, this strain expressed antibacterial activity against Gram-positive bacteria including a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA) EAMC30. Based on these results, the marine sponge-associated isolates represent a novel species of the genus Streptomyces, for which the name Streptomyces poriferorum sp. nov. is proposed, with P01-B04T (= DSM 111306T = CCM 9048T) as the type strain.
ER  -

SANDOVA-POWERS, Megan, Stanislava KRÁLOVÁ, Giang-Son NGUYEN, Dorelle V. FAWWAL, Kristin DEGNES, Anna Sofia LEWIN, Geir KLINKENBERG, Alexander WENTZEL and Mark R. LILES. Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity. \textit{Systematic and Applied Microbiology}. Elsevier GmbH, 2021, vol.~44, No~5, p.~126244-126256. ISSN~0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2021.126244.

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