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@article{1785540,
author = {SandovaandPowers, Megan and Králová, Stanislava and Nguyen, GiangandSon and Fawwal, Dorelle V. and Degnes, Kristin and Lewin, Anna Sofia and Klinkenberg, Geir and Wentzel, Alexander and Liles, Mark R.},
article_number = {5},
doi = {http://dx.doi.org/10.1016/j.syapm.2021.126244},
keywords = {Streptomyces poriferorum sp. nov.; marine sponge; Actinobacteria; polyphasic taxonomy; Trondheim fjord},
language = {eng},
issn = {0723-2020},
journal = {Systematic and Applied Microbiology},
title = {Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity},
url = {https://doi.org/10.1016/j.syapm.2021.126244},
volume = {44},
year = {2021}
}
TY - JOUR
ID - 1785540
AU - Sandova-Powers, Megan - Králová, Stanislava - Nguyen, Giang-Son - Fawwal, Dorelle V. - Degnes, Kristin - Lewin, Anna Sofia - Klinkenberg, Geir - Wentzel, Alexander - Liles, Mark R.
PY - 2021
TI - Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity
JF - Systematic and Applied Microbiology
VL - 44
IS - 5
SP - 126244
EP - 126244
PB - Elsevier GmbH
SN - 07232020
KW - Streptomyces poriferorum sp. nov.
KW - marine sponge
KW - Actinobacteria
KW - polyphasic taxonomy
KW - Trondheim fjord
UR - https://doi.org/10.1016/j.syapm.2021.126244
N2 - Marine sponges represent a rich source of uncharacterized microbial diversity, and many are host to microorganisms that produce biologically active specialized metabolites. Here, a polyphasic approach was used to characterize two Actinobacteria strains, P01-B04T and P01-F02, that were isolated from the marine sponges Geodia barretti (Bowerbank, 1858) and Antho dichotoma (Esper, 1794), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains P01-B04T and P01-F02 are closely related to Streptomyces beijiangensis DSM 41794T, Streptomyces laculatispora NRRL B-24909T, and Streptomyces brevispora NRRL B-24910T. The two strains showed nearly identical 16S rRNA gene sequences (99.93%), and the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) relatedness values were 99.96% and 99.6%, respectively, suggesting that these strains are affiliated with the same species. Chemotaxonomic and culture characteristics of both strains were also consistent with the genus Streptomyces, while phenotypic properties, genome-based comparisons, and phylogenomic analyses distinguished strains P01-B04T and P01-F02 from their closest phylogenetic relatives. In silico analysis predicted that the 8.9 Mb genome of P01-B04T contains at least 41 biosynthetic gene clusters (BGCs) encoding secondary metabolites, indicating that this strain could express diverse bioactive metabolites; in support of this prediction, this strain expressed antibacterial activity against Gram-positive bacteria including a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA) EAMC30. Based on these results, the marine sponge-associated isolates represent a novel species of the genus Streptomyces, for which the name Streptomyces poriferorum sp. nov. is proposed, with P01-B04T (= DSM 111306T = CCM 9048T) as the type strain.
ER -
SANDOVA-POWERS, Megan, Stanislava KRÁLOVÁ, Giang-Son NGUYEN, Dorelle V. FAWWAL, Kristin DEGNES, Anna Sofia LEWIN, Geir KLINKENBERG, Alexander WENTZEL and Mark R. LILES. Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity. \textit{Systematic and Applied Microbiology}. Elsevier GmbH, 2021, vol.~44, No~5, p.~126244-126256. ISSN~0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2021.126244.
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