Histopathology of laboratory-reared Nothobranchius fishes:
Mycobacterial infections versus neoplastic lesions

J 2021

Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions

DYKOVÁ, Iva, Jakub ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ et. al.

Basic information

Original name

Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions

Authors

DYKOVÁ, Iva (203 Czech Republic, guarantor, belonging to the institution), Jakub ŽÁK (203 Czech Republic), Martin REICHARD (203 Czech Republic, belonging to the institution), Kamila SOUČKOVÁ (203 Czech Republic, belonging to the institution), Ondřej SLABÝ (203 Czech Republic, belonging to the institution), Vojtěch BYSTRÝ (203 Czech Republic, belonging to the institution) and Radim BLAŽEK (203 Czech Republic, belonging to the institution)

Edition

Journal of Fish Diseases, Hoboken, Wiley, 2021, 0140-7775

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10617 Marine biology, freshwater biology, limnology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.580

RIV identification code

RIV/00216224:14310/21:00119124

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/jfd.13378

UT WoS

000639234500001

Keywords in English

mononuclear phagocytic system; mycobacterial infections; proliferative lesions; tumour misdiagnosis

Abstract

V originále

Short-lived killifishes of the genus Nothobranchius Peters, 1868 (Cyprinodontiformes) are considered promising model organisms for biomedical research on ageing and tumorigenesis. We conducted histopathological analysis of 411 adult individuals from three Nothobranchius species to study details on spontaneous age-related neoplastic lesions. Light microscopy based on H&E and toluidine blue-stained sections revealed (a) non-proliferative liver changes with pronounced vacuolation of hepatocytes; (b) proliferation of kidney haemopoietic tissue contributing to excretory system damage; (c) proliferation of splenic mononuclear haemoblasts accompanied by reduced erythropoiesis; (d) proliferation of mononuclear cell aggregates in the liver parenchyma; and (e) rare occurrence of hepatocellular adenomas. Ziehl–Neelsen (ZN) staining revealed that the proliferative lesions are a host defence response to mycobacterial infections manifested by activation of the mononuclear phagocytic system and atypical granulomatous inflammatory reaction. 16S rRNA analysis identified three species of Mycobacterium in our samples. Our findings turn attention to lesions which mimic neoplasms by their gross appearance and question the light microscopic interpretation of lesions unless differential ZN staining is included. Beyond the limitations of our morphological approach, the intensity of mycobacterial infections is a challenging opportunity for research into the molecular-genetic background of the mononuclear phagocytic system reaction in Nothobranchius killifish.

Links

GA19-20873S, research and development project

Name: Klíčové mutace v nádorovém genomu Nothobranchius furzeri: od nádorové biologie k návrhu experimentálního modelu sporadické kancerogeneze

Investor: Czech Science Foundation, Driver mutations in cancer genome of Nothobranchius furzeri: from tumor biology to concept of experimental model of spontaneous carcinogenesis

90132, large research infrastructures

Name: NCMG II

Citovat

DYKOVÁ, Iva, Jakub ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ, Vojtěch BYSTRÝ and Radim BLAŽEK. Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions. Journal of Fish Diseases. Hoboken: Wiley, 2021, vol. 44, No 8, p. 1179-1190. ISSN 0140-7775. Available from: https://dx.doi.org/10.1111/jfd.13378.

@article{1785678,
   author = {Dyková, Iva and Žák, Jakub and Reichard, Martin and Součková, Kamila and Slabý, Ondřej and Bystrý, Vojtěch and Blažek, Radim},
   article_location = {Hoboken},
   article_number = {8},
   doi = {http://dx.doi.org/10.1111/jfd.13378},
   keywords = {mononuclear phagocytic system; mycobacterial infections; proliferative lesions; tumour misdiagnosis},
   language = {eng},
   issn = {0140-7775},
   journal = {Journal of Fish Diseases},
   title = {Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions},
   url = {https://doi.org/10.1111/jfd.13378},
   volume = {44},
   year = {2021}
}

TY  - JOUR
ID  - 1785678
AU  - Dyková, Iva - Žák, Jakub - Reichard, Martin - Součková, Kamila - Slabý, Ondřej - Bystrý, Vojtěch - Blažek, Radim
PY  - 2021
TI  - Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions
JF  - Journal of Fish Diseases
VL  - 44
IS  - 8
SP  - 1179-1190
EP  - 1179-1190
PB  - Wiley
SN  - 01407775
KW  - mononuclear phagocytic system
KW  - mycobacterial infections
KW  - proliferative lesions
KW  - tumour misdiagnosis
UR  - https://doi.org/10.1111/jfd.13378
N2  - Short-lived killifishes of the genus Nothobranchius Peters, 1868 (Cyprinodontiformes) are considered promising model organisms for biomedical research on ageing and tumorigenesis. We conducted histopathological analysis of 411 adult individuals from three Nothobranchius species to study details on spontaneous age-related neoplastic lesions. Light microscopy based on H&E and toluidine blue-stained sections revealed (a) non-proliferative liver changes with pronounced vacuolation of hepatocytes; (b) proliferation of kidney haemopoietic tissue contributing to excretory system damage; (c) proliferation of splenic mononuclear haemoblasts accompanied by reduced erythropoiesis; (d) proliferation of mononuclear cell aggregates in the liver parenchyma; and (e) rare occurrence of hepatocellular adenomas. Ziehl–Neelsen (ZN) staining revealed that the proliferative lesions are a host defence response to mycobacterial infections manifested by activation of the mononuclear phagocytic system and atypical granulomatous inflammatory reaction. 16S rRNA analysis identified three species of Mycobacterium in our samples. Our findings turn attention to lesions which mimic neoplasms by their gross appearance and question the light microscopic interpretation of lesions unless differential ZN staining is included. Beyond the limitations of our morphological approach, the intensity of mycobacterial infections is a challenging opportunity for research into the molecular-genetic background of the mononuclear phagocytic system reaction in Nothobranchius killifish.
ER  -

DYKOVÁ, Iva, Jakub ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ, Vojtěch BYSTRÝ and Radim BLAŽEK. Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions. \textit{Journal of Fish Diseases}. Hoboken: Wiley, 2021, vol.~44, No~8, p.~1179-1190. ISSN~0140-7775. Available from: https://dx.doi.org/10.1111/jfd.13378.

Detailed Information on Publication Record