Swim bladder as a primary site of mycobacterial infection in
Nothobranchius 'belly sliders'

J 2021

Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'

DYKOVÁ, Iva, J. ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ et. al.

Basic information

Original name

Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'

Authors

DYKOVÁ, Iva (203 Czech Republic, guarantor, belonging to the institution), J. ŽÁK, Martin REICHARD (203 Czech Republic, belonging to the institution), Kamila SOUČKOVÁ (203 Czech Republic, belonging to the institution), Ondřej SLABÝ (203 Czech Republic, belonging to the institution) and Radim BLAŽEK (203 Czech Republic, belonging to the institution)

Edition

Diseases of aquatic organisms, Inter Research, 2021, 0177-5103

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10618 Ecology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.769

RIV identification code

RIV/00216224:14310/21:00119125

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3354/dao03601

UT WoS

000691780800010

Keywords in English

Abnormal swimming; Laboratory-reared killifish; Model organism; Mycobacterial infection

Abstract

V originále

The swim bladder inflates early after fish hatching via its interconnection with the digestive tract (ductus pneumaticus). This interconnection may serve as a portal to foreign particles, including bacteria, causing deficiencies in primary swim bladder inflation. We histologically examined 134 African annual killifish (genus Nothobranchius) with secondary loss of swim bladder function ('belly sliders'). We demonstrate that these fish lost the ability of air regulation in their swim bladders likely due to Mycobacterium spp. infection at an individual-specific age. Nearly all examined belly sliders had thickened swim bladder walls, and their swim bladder was filled with material containing mycobacteria, cell debris, young monocytic cells and phagocyting macrophages. Mycobacterial infection was restricted to the swim bladder in juveniles, where mycobacteria likely enter the host through the ductus pneumaticus. Infection in adults was systemic and mycobacteria were present in all examined organs. Presence of mycobacteria in the epithelial lining and submucosal layers of the digestive tract of adults suggests that it may also serve as the entrance site of infection. We suspect 2 sources of Mycobacterium contamination: dietary (with bloodworms) and/or contaminated hatching substrate. These sources of contamination may be eliminated by use of laboratory dry feed and egg disinfection prior to hatching.

Links

GA19-20873S, research and development project

Name: Klíčové mutace v nádorovém genomu Nothobranchius furzeri: od nádorové biologie k návrhu experimentálního modelu sporadické kancerogeneze

Investor: Czech Science Foundation, Driver mutations in cancer genome of Nothobranchius furzeri: from tumor biology to concept of experimental model of spontaneous carcinogenesis

Citovat

DYKOVÁ, Iva, J. ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ and Radim BLAŽEK. Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'. Diseases of aquatic organisms. Inter Research, 2021, vol. 145, July, p. 111-117. ISSN 0177-5103. Available from: https://dx.doi.org/10.3354/dao03601.

@article{1785687,
   author = {Dyková, Iva and Žák, J. and Reichard, Martin and Součková, Kamila and Slabý, Ondřej and Blažek, Radim},
   article_number = {July},
   doi = {http://dx.doi.org/10.3354/dao03601},
   keywords = {Abnormal swimming; Laboratory-reared killifish; Model organism; Mycobacterial infection},
   language = {eng},
   issn = {0177-5103},
   journal = {Diseases of aquatic organisms},
   title = {Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'},
   url = {https://doi.org/10.3354/dao03601},
   volume = {145},
   year = {2021}
}

TY  - JOUR
ID  - 1785687
AU  - Dyková, Iva - Žák, J. - Reichard, Martin - Součková, Kamila - Slabý, Ondřej - Blažek, Radim
PY  - 2021
TI  - Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'
JF  - Diseases of aquatic organisms
VL  - 145
IS  - July
SP  - 111-117
EP  - 111-117
PB  - Inter Research
SN  - 01775103
KW  - Abnormal swimming
KW  - Laboratory-reared killifish
KW  - Model organism
KW  - Mycobacterial infection
UR  - https://doi.org/10.3354/dao03601
N2  - The swim bladder inflates early after fish hatching via its interconnection with the digestive tract (ductus pneumaticus). This interconnection may serve as a portal to foreign particles, including bacteria, causing deficiencies in primary swim bladder inflation. We histologically examined 134 African annual killifish (genus Nothobranchius) with secondary loss of swim bladder function ('belly sliders'). We demonstrate that these fish lost the ability of air regulation in their swim bladders likely due to Mycobacterium spp. infection at an individual-specific age. Nearly all examined belly sliders had thickened swim bladder walls, and their swim bladder was filled with material containing mycobacteria, cell debris, young monocytic cells and phagocyting macrophages. Mycobacterial infection was restricted to the swim bladder in juveniles, where mycobacteria likely enter the host through the ductus pneumaticus. Infection in adults was systemic and mycobacteria were present in all examined organs. Presence of mycobacteria in the epithelial lining and submucosal layers of the digestive tract of adults suggests that it may also serve as the entrance site of infection. We suspect 2 sources of Mycobacterium contamination: dietary (with bloodworms) and/or contaminated hatching substrate. These sources of contamination may be eliminated by use of laboratory dry feed and egg disinfection prior to hatching.
ER  -

DYKOVÁ, Iva, J. ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ and Radim BLAŽEK. Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'. \textit{Diseases of aquatic organisms}. Inter Research, 2021, vol.~145, July, p.~111-117. ISSN~0177-5103. Available from: https://dx.doi.org/10.3354/dao03601.

Detailed Information on Publication Record