Detailed Information on Publication Record
2021
Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria
OBOŘILOVÁ, Radka, Hana ŠIMEČKOVÁ, Matěj PASTUCHA, Šimon KLIMOVIČ, Ivana VÍŠOVÁ et. al.Basic information
Original name
Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria
Authors
OBOŘILOVÁ, Radka (203 Czech Republic, belonging to the institution), Hana ŠIMEČKOVÁ (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Šimon KLIMOVIČ (203 Czech Republic, belonging to the institution), Ivana VÍŠOVÁ (203 Czech Republic), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Hana VAISOCHEROVÁ-LÍSALOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution)
Edition
Nanoscale, Cambridge, Royal Society of Chemistry, 2021, 2040-3364
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 8.307
RIV identification code
RIV/00216224:14310/21:00119126
Organization unit
Faculty of Science
UT WoS
000680101900001
Keywords in English
Atomic force microscopy; AFM; Surface plasmon resonance; SPR; Staphylococcus aureus; Lysis; Lysostaphin; Bacteriophage; Phage therapy
Tags
International impact, Reviewed
Změněno: 15/10/2024 09:00, Ing. Monika Szurmanová, Ph.D.
Abstract
V originále
The growing incidence of multidrug-resistant bacterial strains presents a major challenge in modern medicine. Antibiotic resistance is often exhibited by Staphylococcus aureus, which causes severe infections in human and animal hosts and leads to significant economic losses. Antimicrobial agents with enzymatic activity (enzybiotics) and phage therapy represent promising and effective alternatives to classic antibiotics. However, new tools are needed to study phage–bacteria interactions and bacterial lysis with high resolution and in real-time. Here, we introduce a method for studying the lysis of S. aureus at the single-cell level in real-time using atomic force microscopy (AFM) in liquid. We demonstrate the ability of the method to monitor the effect of the enzyme lysostaphin on S. aureus and the lytic action of the Podoviridae phage P68. AFM allowed the topographic and biomechanical properties of individual bacterial cells to be monitored at high resolution over the course of their lysis, under near-physiological conditions. Changes in the stiffness of S. aureus cells during lysis were studied by analyzing force–distance curves to determine Young's modulus. This allowed observing a progressive decline in cellular stiffness corresponding to the disintegration of the cell envelope. The AFM experiments were complemented by surface plasmon resonance (SPR) experiments that provided information on the kinetics of phage-bacterium binding and the subsequent lytic processes. This approach forms the foundation of an innovative framework for studying the lysis of individual bacteria that may facilitate the further development of phage therapy.
Links
| GA18-13064S, research and development project |
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| GA21-03156S, research and development project |
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| LM2018127, research and development project |
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| LQ1601, research and development project |
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| LTAB19011, research and development project |
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| 90127, large research infrastructures |
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