OBOŘILOVÁ, Radka, Hana ŠIMEČKOVÁ, Matěj PASTUCHA, Šimon KLIMOVIČ, Ivana VÍŠOVÁ, Jan PŘIBYL, Hana VAISOCHEROVÁ-LÍSALOVÁ, Roman PANTŮČEK, Petr SKLÁDAL, Ivana MAŠLAŇOVÁ and Zdeněk FARKA. Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria. Nanoscale. Cambridge: Royal Society of Chemistry, 2021, vol. 13, No 31, p. 13538-13549. ISSN 2040-3364. Available from: https://dx.doi.org/10.1039/D1NR02921E.
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Basic information
Original name Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria
Authors OBOŘILOVÁ, Radka (203 Czech Republic, belonging to the institution), Hana ŠIMEČKOVÁ (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Šimon KLIMOVIČ (203 Czech Republic, belonging to the institution), Ivana VÍŠOVÁ (203 Czech Republic), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Hana VAISOCHEROVÁ-LÍSALOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution).
Edition Nanoscale, Cambridge, Royal Society of Chemistry, 2021, 2040-3364.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10608 Biochemistry and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 8.307
RIV identification code RIV/00216224:14310/21:00119126
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1039/D1NR02921E
UT WoS 000680101900001
Keywords in English Atomic force microscopy; AFM; Surface plasmon resonance; SPR; Staphylococcus aureus; Lysis; Lysostaphin; Bacteriophage; Phage therapy
Tags CF NANO, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 10/2/2023 12:05.
Abstract
The growing incidence of multidrug-resistant bacterial strains presents a major challenge in modern medicine. Antibiotic resistance is often exhibited by Staphylococcus aureus, which causes severe infections in human and animal hosts and leads to significant economic losses. Antimicrobial agents with enzymatic activity (enzybiotics) and phage therapy represent promising and effective alternatives to classic antibiotics. However, new tools are needed to study phage–bacteria interactions and bacterial lysis with high resolution and in real-time. Here, we introduce a method for studying the lysis of S. aureus at the single-cell level in real-time using atomic force microscopy (AFM) in liquid. We demonstrate the ability of the method to monitor the effect of the enzyme lysostaphin on S. aureus and the lytic action of the Podoviridae phage P68. AFM allowed the topographic and biomechanical properties of individual bacterial cells to be monitored at high resolution over the course of their lysis, under near-physiological conditions. Changes in the stiffness of S. aureus cells during lysis were studied by analyzing force–distance curves to determine Young's modulus. This allowed observing a progressive decline in cellular stiffness corresponding to the disintegration of the cell envelope. The AFM experiments were complemented by surface plasmon resonance (SPR) experiments that provided information on the kinetics of phage-bacterium binding and the subsequent lytic processes. This approach forms the foundation of an innovative framework for studying the lysis of individual bacteria that may facilitate the further development of phage therapy.
Links
GA18-13064S, research and development projectName: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus
Investor: Czech Science Foundation
GA21-03156S, research and development projectName: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů
Investor: Czech Science Foundation
LM2018127, research and development projectName: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
LTAB19011, research and development projectName: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel
Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION
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