Detailed Information on Publication Record
2021
Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.
SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Roman SOBOTKA, Hans-Jürgen BUSSE, Pavel ŠVEC et. al.Basic information
Original name
Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.
Authors
SEDLÁČEK, Ivo (203 Czech Republic, guarantor, belonging to the institution), Pavla HOLOCHOVÁ (203 Czech Republic, belonging to the institution), Roman SOBOTKA, Hans-Jürgen BUSSE, Pavel ŠVEC (203 Czech Republic, belonging to the institution), Stanislava KRÁLOVÁ (703 Slovakia, belonging to the institution), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Jan PILNÝ, Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution), Vendula KOUBLOVÁ (203 Czech Republic, belonging to the institution) and Karel SEDLÁŘ
Edition
Microbiology Spectrum, American Society for Microbiology, 2021, 2165-0497
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 9.043
RIV identification code
RIV/00216224:14310/21:00122085
Organization unit
Faculty of Science
UT WoS
000703076500085
Keywords in English
psychrophiles; Rugamonas; taxonomy; Antarctica; violacein; species description
Tags
International impact, Reviewed
Změněno: 10/10/2024 14:41, Ing. Martina Blahová
Abstract
V originále
A group of 11 bacterial strains was isolated from streams and lakes located in a deglaciated northern part of James Ross Island, Antarctica. They were rod-shaped, Gram-stain-negative, motile, and catalase-positive and produced blue-violet-pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, automated ribotyping, repetitive element sequence-based PCR (rep-PCR), MALDI-TOF MS, fatty acid profile, chemotaxonomy analyses, and extensive biotyping was applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all the isolates constituted a coherent group belonging to the genus Rugamonas. The closest relatives to the representative isolate P5900T were Rugamonas rubra CCM 3730T, Rugamonas rivuli FT103WT, and Rugamonas aquatica FT29WT, exhibiting 99.2%, 99.1%, and 98.6% 16S rRNA pairwise similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values calculated from the whole-genome sequencing data clearly proved that P5900T represents a distinct Rugamonas species. The G+C content of genomic DNAs was 66.1 mol%. The major components in fatty acid profiles were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C 16:0, and C12:0. The cellular quinone content contained exclusively ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The polyamine pattern was composed of putrescine, 2-hydroxputrescine, and spermidine.
Links
LM2015078, research and development project |
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LM2018127, research and development project |
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90127, large research infrastructures |
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