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@inbook{1786517,
author = {Kokkonen, Piia Pauliina and Koudeláková, Táňa and Chaloupková, Radka and Daniel, Lukáš and Prokop, Zbyněk and Damborský, Jiří},
address = {Cham},
booktitle = {Aerobic Utilization of Hydrocarbons, Oils, and Lipids},
doi = {http://dx.doi.org/10.1007/978-3-319-50418-6_15},
keywords = {haloalkane dehalogenase; protein engineering},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Cham},
isbn = {978-3-319-50417-9},
pages = {367-387},
publisher = {Springer},
title = {Structure-Function Relationships and Engineering of Haloalkane Dehalogenases},
url = {https://doi.org/10.1007/978-3-319-50418-6_15},
year = {2019}
}
TY - CHAP
ID - 1786517
AU - Kokkonen, Piia Pauliina - Koudeláková, Táňa - Chaloupková, Radka - Daniel, Lukáš - Prokop, Zbyněk - Damborský, Jiří
PY - 2019
TI - Structure-Function Relationships and Engineering of Haloalkane Dehalogenases
VL - 1.
PB - Springer
CY - Cham
SN - 9783319504179
KW - haloalkane dehalogenase
KW - protein engineering
UR - https://doi.org/10.1007/978-3-319-50418-6_15
N2 - The structure-function relationships for haloalkane dehalogenases, one of the best characterized enzyme families involved in degradation of halogenated compounds, are described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified (i) activity and specificity, (ii) stability, and (ii) enantioselectivity. Many variants carried mutations in the tunnels connecting the buried active site with surrounding solvent, rather than in the active site itself. Mutagenesis of residues lining the protein tunnels represents attractive and a viable approach of protein engineering.
ER -
KOKKONEN, Piia Pauliina, Táňa KOUDELÁKOVÁ, Radka CHALOUPKOVÁ, Lukáš DANIEL, Zbyněk PROKOP and Jiří DAMBORSKÝ. Structure-Function Relationships and Engineering of Haloalkane Dehalogenases. In \textit{Aerobic Utilization of Hydrocarbons, Oils, and Lipids}. Cham: Springer, 2019, p.~367-387. 1. ISBN~978-3-319-50417-9. Available from: https://dx.doi.org/10.1007/978-3-319-50418-6\_{}15.
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