Detailed Information on Publication Record
2021
Eudiplozoon nipponicum: morphofunctional adaptations of diplozoid monogeneans for confronting their host
BARDŮNEK VALIGUROVÁ, Andrea, Naděžda VAŠKOVICOVÁ, Milan GELNAR, Magdaléna KOVÁČIKOVÁ, Iveta HODOVÁ et. al.Basic information
Original name
Eudiplozoon nipponicum: morphofunctional adaptations of diplozoid monogeneans for confronting their host
Authors
BARDŮNEK VALIGUROVÁ, Andrea (703 Slovakia, guarantor, belonging to the institution), Naděžda VAŠKOVICOVÁ (203 Czech Republic), Milan GELNAR (203 Czech Republic, belonging to the institution), Magdaléna KOVÁČIKOVÁ (703 Slovakia, belonging to the institution) and Iveta HODOVÁ (203 Czech Republic, belonging to the institution)
Edition
BMC Zoology, London, BMC, 2021, 2056-3132
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10613 Zoology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.769
RIV identification code
RIV/00216224:14310/21:00119141
Organization unit
Faculty of Science
UT WoS
000686671800001
Keywords in English
host-parasite interactions; tegument; musculature; nervous system; sensory structures; excretory system; secretion; ultrastructure; immunofluorescence; freeze-etching
Tags
Tags
International impact, Reviewed
Změněno: 13/9/2021 15:35, Mgr. Marie Šípková, DiS.
Abstract
V originále
Monogeneans, in general, show a range of unique adaptations to a parasitic lifestyle, making this group enormously diverse. Due to their unique biological properties, diplozoid monogeneans represent an attractive model group for various investigations on diverse biological interactions. However, despite numerous studies, there are still gaps in our knowledge of diplozoid biology and morphofunctional adaptations. In this study, we provide a comprehensive microscopic analysis of systems/structures involved in niche searching, sensing and self-protection against the host environment, and excretory/secretory processes in Eudiplozoon nipponicum. Freeze-etching enabled us to detect syncytium organisational features not visible by TEM alone, such as the presence of a membrane subjacent to the apical plasma membrane (separated by a dense protein layer) and a lack of basal plasma membrane. We located several types of secretory/excretory vesicles and bodies, including those attached to the superficial membranes of the tegument. Giant unicellular glands were seen accumulating predominantly in the apical forebody and hindbody haptor region. Muscle layer organisation differed from that generally described, with the outer circular and inner longitudinal muscles being basket-like interwoven by diagonal muscles with additional perpendicular muscles anchored to the tegument. Abundant muscles within the tegumentary ridges were detected, which presumably assist in fixing the parasite between the gill lamellae. Freeze-etching, alongside transmission electron and confocal microscopy with tubulin labelling, enabled visualisation of the protonephridia and nervous system, including the peripheral network and receptor innervation. Three types of receptor were identified: 1) uniciliated sensory endings with a subtle (or missing) tegumentary rim, 2) obviously raised uniciliated receptors with a prominent tegumentary rim (packed with massive innervation and muscles) and 3) non-ciliated papillae (restricted to the hindbody lateral region). This study points to specific morphofunctional adaptations that have evolved in diplozoid monogeneans to confront their fish host. We clearly demonstrate that the combination of different microscopic techniques is beneficial and can reveal hidden differences, even in much-studied model organisms such as E. nipponicum.
Links
| GBP505/12/G112, research and development project |
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