| CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ, K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG and J. B. PARR. Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres. The Journal of Infectious Diseases. Chicago: University of Chicago Press, 2021, vol. 223, No 5, p. 848-853. ISSN 0022-1899. Available from: https://dx.doi.org/10.1093/infdis/jiaa449. |
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@article{1786839,
author = {Chen, W. T. and Šmajs, David and Hu, Y. F. and Ke, W. J. and Pospíšilová, Petra and Hawley, K. L. and Caimano, M. J. and Radolf, J. D. and Sena, A. and Tucker, J. D. and Yang, B. and Juliano, J. J. and Zheng, H. P. and Parr, J. B.},
article_location = {Chicago},
article_number = {5},
doi = {http://dx.doi.org/10.1093/infdis/jiaa449},
keywords = {chancre; China; syphilis; Treponema pallidum; whole-genome sequencing},
language = {eng},
issn = {0022-1899},
journal = {The Journal of Infectious Diseases},
title = {Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres},
url = {https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext},
volume = {223},
year = {2021}
}
TY - JOUR
ID - 1786839
AU - Chen, W. T. - Šmajs, David - Hu, Y. F. - Ke, W. J. - Pospíšilová, Petra - Hawley, K. L. - Caimano, M. J. - Radolf, J. D. - Sena, A. - Tucker, J. D. - Yang, B. - Juliano, J. J. - Zheng, H. P. - Parr, J. B.
PY - 2021
TI - Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres
JF - The Journal of Infectious Diseases
VL - 223
IS - 5
SP - 848-853
EP - 848-853
PB - University of Chicago Press
SN - 00221899
KW - chancre
KW - China
KW - syphilis
KW - Treponema pallidum
KW - whole-genome sequencing
UR - https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext
N2 - Background. Whole-genome sequencing (WGS) of lreponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. Methods. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. Conclusions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
ER -
CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ, K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG and J. B. PARR. Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres. \textit{The Journal of Infectious Diseases}. Chicago: University of Chicago Press, 2021, vol.~223, No~5, p.~848-853. ISSN~0022-1899. Available from: https://dx.doi.org/10.1093/infdis/jiaa449.
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