Analysis of Treponema pallidum Strains From China Using
Improved Methods for Whole-Genome Sequencing From Primary
Syphilis Chancres

J 2021

Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres

CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ et. al.

Basic information

Original name

Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres

Authors

CHEN, W. T., David ŠMAJS (203 Czech Republic, belonging to the institution), Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG and J. B. PARR (guarantor)

Edition

The Journal of Infectious Diseases, Chicago, University of Chicago Press, 2021, 0022-1899

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.759

RIV identification code

RIV/00216224:14110/21:00119144

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1093/infdis/jiaa449

UT WoS

000648907800016

Keywords in English

chancre; China; syphilis; Treponema pallidum; whole-genome sequencing

Abstract

V originále

Background. Whole-genome sequencing (WGS) of lreponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. Methods. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. Conclusions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.

Links

GA17-25455S, research and development project

Name: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Czech Science Foundation

U19 AI144177, interní kód MU

Name: A global syphilis vaccine targeting outer membrane proteins of Treponema pallidum

Investor: Ostatní - foreign

Citovat

CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ, K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG and J. B. PARR. Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres. The Journal of Infectious Diseases. Chicago: University of Chicago Press, 2021, vol. 223, No 5, p. 848-853. ISSN 0022-1899. Available from: https://dx.doi.org/10.1093/infdis/jiaa449.

@article{1786839,
   author = {Chen, W. T. and Šmajs, David and Hu, Y. F. and Ke, W. J. and Pospíšilová, Petra and Hawley, K. L. and Caimano, M. J. and Radolf, J. D. and Sena, A. and Tucker, J. D. and Yang, B. and Juliano, J. J. and Zheng, H. P. and Parr, J. B.},
   article_location = {Chicago},
   article_number = {5},
   doi = {http://dx.doi.org/10.1093/infdis/jiaa449},
   keywords = {chancre; China; syphilis; Treponema pallidum; whole-genome sequencing},
   language = {eng},
   issn = {0022-1899},
   journal = {The Journal of Infectious Diseases},
   title = {Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres},
   url = {https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext},
   volume = {223},
   year = {2021}
}

TY  - JOUR
ID  - 1786839
AU  - Chen, W. T. - Šmajs, David - Hu, Y. F. - Ke, W. J. - Pospíšilová, Petra - Hawley, K. L. - Caimano, M. J. - Radolf, J. D. - Sena, A. - Tucker, J. D. - Yang, B. - Juliano, J. J. - Zheng, H. P. - Parr, J. B.
PY  - 2021
TI  - Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres
JF  - The Journal of Infectious Diseases
VL  - 223
IS  - 5
SP  - 848-853
EP  - 848-853
PB  - University of Chicago Press
SN  - 00221899
KW  - chancre
KW  - China
KW  - syphilis
KW  - Treponema pallidum
KW  - whole-genome sequencing
UR  - https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext
N2  - Background. Whole-genome sequencing (WGS) of lreponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. Methods. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. Conclusions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
ER  -

CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ, K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG and J. B. PARR. Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres. \textit{The Journal of Infectious Diseases}. Chicago: University of Chicago Press, 2021, vol.~223, No~5, p.~848-853. ISSN~0022-1899. Available from: https://dx.doi.org/10.1093/infdis/jiaa449.

Detailed Information on Publication Record