CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ, K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG and J. B. PARR. Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres. The Journal of Infectious Diseases. Chicago: University of Chicago Press, 2021, vol. 223, No 5, p. 848-853. ISSN 0022-1899. Available from: https://dx.doi.org/10.1093/infdis/jiaa449. |
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@article{1786839, author = {Chen, W. T. and Šmajs, David and Hu, Y. F. and Ke, W. J. and Pospíšilová, Petra and Hawley, K. L. and Caimano, M. J. and Radolf, J. D. and Sena, A. and Tucker, J. D. and Yang, B. and Juliano, J. J. and Zheng, H. P. and Parr, J. B.}, article_location = {Chicago}, article_number = {5}, doi = {http://dx.doi.org/10.1093/infdis/jiaa449}, keywords = {chancre; China; syphilis; Treponema pallidum; whole-genome sequencing}, language = {eng}, issn = {0022-1899}, journal = {The Journal of Infectious Diseases}, title = {Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres}, url = {https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext}, volume = {223}, year = {2021} }
TY - JOUR ID - 1786839 AU - Chen, W. T. - Šmajs, David - Hu, Y. F. - Ke, W. J. - Pospíšilová, Petra - Hawley, K. L. - Caimano, M. J. - Radolf, J. D. - Sena, A. - Tucker, J. D. - Yang, B. - Juliano, J. J. - Zheng, H. P. - Parr, J. B. PY - 2021 TI - Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres JF - The Journal of Infectious Diseases VL - 223 IS - 5 SP - 848-853 EP - 848-853 PB - University of Chicago Press SN - 00221899 KW - chancre KW - China KW - syphilis KW - Treponema pallidum KW - whole-genome sequencing UR - https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext N2 - Background. Whole-genome sequencing (WGS) of lreponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. Methods. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. Conclusions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China. ER -
CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ, K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG and J. B. PARR. Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres. \textit{The Journal of Infectious Diseases}. Chicago: University of Chicago Press, 2021, vol.~223, No~5, p.~848-853. ISSN~0022-1899. Available from: https://dx.doi.org/10.1093/infdis/jiaa449.
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