RNA-seq Characterization of Melanoma Phenotype Switch in 3D
Collagen after p38 MAPK Inhibitor Treatment

J 2021

RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment

CERMAK, Vladimir; Aneta SKARKOVA; Ladislav MERTA; Veronika KOLOMAZNIKOVA; Veronika PALUŠOVÁ et. al.

Basic information

Original name

RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment

Authors

CERMAK, Vladimir (203 Czech Republic); Aneta SKARKOVA (203 Czech Republic); Ladislav MERTA (203 Czech Republic); Veronika KOLOMAZNIKOVA (203 Czech Republic); Veronika PALUŠOVÁ (703 Slovakia, belonging to the institution); Stjepan ULDRIJAN (203 Czech Republic, belonging to the institution); Daniel ROSEL (203 Czech Republic) and Jan BRABEK (203 Czech Republic)

Edition

Biomolecules, BASEL, MDPI, 2021, 2218-273X

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 6.064

RIV identification code

RIV/00216224:14110/21:00122131

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/biom11030449

UT WoS

000633403200001

EID Scopus

2-s2.0-85102562625

Keywords in English

cancer; melanoma; metastasis; phenotype switch; amoeboid invasion

Abstract

V originále

Melanoma phenotype plasticity underlies tumour dissemination and resistance to therapy, yet its regulation is incompletely understood. In vivo switching between a more differentiated, proliferative phenotype and a dedifferentiated, invasive phenotype is directed by the tumour microenvironment. We found that treatment of partially dedifferentiated, invasive A375M2 cells with two structurally unrelated p38 MAPK inhibitors, SB2021920 and BIRB796, induces a phenotype switch in 3D collagen, as documented by increased expression of melanocyte differentiation markers and a loss of invasive phenotype markers. The phenotype is accompanied by morphological change corresponding to amoeboid-mesenchymal transition. We performed RNA sequencing with an Illumina HiSeq platform to fully characterise transcriptome changes underlying the switch. Gene expression results obtained with RNA-seq were validated by comparing them with RT-qPCR. Transcriptomic data generated in the study will extend the present understanding of phenotype plasticity in melanoma and its contribution to invasion and metastasis.

Cite

CERMAK, Vladimir; Aneta SKARKOVA; Ladislav MERTA; Veronika KOLOMAZNIKOVA; Veronika PALUŠOVÁ; Stjepan ULDRIJAN; Daniel ROSEL and Jan BRABEK. RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment. Biomolecules. BASEL: MDPI, 2021, vol. 11, No 3, p. 1-13. ISSN 2218-273X. Available from: https://dx.doi.org/10.3390/biom11030449.

@article{1786886,
   author = {Cermak, Vladimir and Skarkova, Aneta and Merta, Ladislav and Kolomaznikova, Veronika and Palušová, Veronika and Uldrijan, Stjepan and Rosel, Daniel and Brabek, Jan},
   article_location = {BASEL},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/biom11030449},
   keywords = {cancer; melanoma; metastasis; phenotype switch; amoeboid invasion},
   language = {eng},
   issn = {2218-273X},
   journal = {Biomolecules},
   title = {RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment},
   url = {https://www.mdpi.com/2218-273X/11/3/449},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1786886
AU  - Cermak, Vladimir - Skarkova, Aneta - Merta, Ladislav - Kolomaznikova, Veronika - Palušová, Veronika - Uldrijan, Stjepan - Rosel, Daniel - Brabek, Jan
PY  - 2021
TI  - RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment
JF  - Biomolecules
VL  - 11
IS  - 3
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 2218273X
KW  - cancer
KW  - melanoma
KW  - metastasis
KW  - phenotype switch
KW  - amoeboid invasion
UR  - https://www.mdpi.com/2218-273X/11/3/449
N2  - Melanoma phenotype plasticity underlies tumour dissemination and resistance to therapy, yet its regulation is incompletely understood. In vivo switching between a more differentiated, proliferative phenotype and a dedifferentiated, invasive phenotype is directed by the tumour microenvironment. We found that treatment of partially dedifferentiated, invasive A375M2 cells with two structurally unrelated p38 MAPK inhibitors, SB2021920 and BIRB796, induces a phenotype switch in 3D collagen, as documented by increased expression of melanocyte differentiation markers and a loss of invasive phenotype markers. The phenotype is accompanied by morphological change corresponding to amoeboid-mesenchymal transition. We performed RNA sequencing with an Illumina HiSeq platform to fully characterise transcriptome changes underlying the switch. Gene expression results obtained with RNA-seq were validated by comparing them with RT-qPCR. Transcriptomic data generated in the study will extend the present understanding of phenotype plasticity in melanoma and its contribution to invasion and metastasis.
ER  -

CERMAK, Vladimir; Aneta SKARKOVA; Ladislav MERTA; Veronika KOLOMAZNIKOVA; Veronika PALUŠOVÁ; Stjepan ULDRIJAN; Daniel ROSEL and Jan BRABEK. RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment. \textit{Biomolecules}. BASEL: MDPI, 2021, vol.~11, No~3, p.~1-13. ISSN~2218-273X. Available from: https://dx.doi.org/10.3390/biom11030449.

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