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@article{1787377,
author = {Peltanová, Barbora and Lišková, Markéta and Gumulec, Jaromír and Raudenská, Martina and Holcová Polanská, Hana and Vaculovič, Tomáš and Kalfert, David and Grega, Marek and Plzak, Jan and Betka, Jan and Masařík, Michal},
article_location = {Basel},
article_number = {4},
doi = {http://dx.doi.org/10.3390/ijms22041912},
keywords = {head and neck cancer; cancer-associated fibroblasts; cisplatin; treatment resistance; cancer recurrence; patient-derived cell cultures; coculture},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts},
url = {https://www.mdpi.com/1422-0067/22/4/1912},
volume = {22},
year = {2021}
}
TY - JOUR
ID - 1787377
AU - Peltanová, Barbora - Lišková, Markéta - Gumulec, Jaromír - Raudenská, Martina - Holcová Polanská, Hana - Vaculovič, Tomáš - Kalfert, David - Grega, Marek - Plzak, Jan - Betka, Jan - Masařík, Michal
PY - 2021
TI - Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts
JF - International Journal of Molecular Sciences
VL - 22
IS - 4
SP - 1-13
EP - 1-13
PB - MDPI
SN - 14220067
KW - head and neck cancer
KW - cancer-associated fibroblasts
KW - cisplatin
KW - treatment resistance
KW - cancer recurrence
KW - patient-derived cell cultures
KW - coculture
UR - https://www.mdpi.com/1422-0067/22/4/1912
N2 - Cancer-associated fibroblasts (CAFs) are one of the most abundant and critical components of the tumor stroma. CAFs can impact many important steps of cancerogenesis and may also influence treatment resistance. Some of these effects need the direct contact of CAFs and cancer cells, while some involve paracrine signals. In this study, we investigated the ability of head and neck squamous cell carcinomas (HNSCC) patient-derived CAFs to promote or inhibit the colony-forming ability of HNSCC cells. The effect of cisplatin on this promoting or inhibiting influence was also studied. The subsequent analysis focused on changes in the expression of genes associated with cancer progression. We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different "sensitizing ratio". Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance. On the other hand, CCL2 expression was associated with sensitizing effect. Significantly higher amounts of cisplatin were found in CAFs derived from patients who subsequently experienced a recurrence. In conclusion, our results showed that CAFs could promote and/or inhibit colony-forming capability and cisplatin resistance in HNSCC cells via paracrine effects and subsequent changes in gene expression of cancer-associated genes in cancer cells.
ER -
PELTANOVÁ, Barbora, Markéta LIŠKOVÁ, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana HOLCOVÁ POLANSKÁ, Tomáš VACULOVIČ, David KALFERT, Marek GREGA, Jan PLZAK, Jan BETKA a Michal MASAŘÍK. Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts. \textit{International Journal of Molecular Sciences}. Basel: MDPI, roč.~22, č.~4, s.~1-13. ISSN~1422-0067. doi:10.3390/ijms22041912. 2021.
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