Sensitivity to Cisplatin in Head and Neck Cancer Cells Is
Significantly Affected by Patient-Derived Cancer-Associated
Fibroblasts

J 2021

Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts

PELTANOVÁ, Barbora, Markéta LIŠKOVÁ, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana HOLCOVÁ POLANSKÁ et. al.

Basic information

Original name

Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts

Authors

PELTANOVÁ, Barbora (203 Czech Republic, belonging to the institution), Markéta LIŠKOVÁ (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Hana HOLCOVÁ POLANSKÁ (203 Czech Republic, belonging to the institution), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), David KALFERT (203 Czech Republic), Marek GREGA (203 Czech Republic), Jan PLZAK (203 Czech Republic), Jan BETKA (203 Czech Republic) and Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Molecular Sciences, Basel, MDPI, 2021, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.208

RIV identification code

RIV/00216224:14110/21:00119149

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms22041912

UT WoS

000623863700001

Keywords in English

head and neck cancer; cancer-associated fibroblasts; cisplatin; treatment resistance; cancer recurrence; patient-derived cell cultures; coculture

Abstract

V originále

Cancer-associated fibroblasts (CAFs) are one of the most abundant and critical components of the tumor stroma. CAFs can impact many important steps of cancerogenesis and may also influence treatment resistance. Some of these effects need the direct contact of CAFs and cancer cells, while some involve paracrine signals. In this study, we investigated the ability of head and neck squamous cell carcinomas (HNSCC) patient-derived CAFs to promote or inhibit the colony-forming ability of HNSCC cells. The effect of cisplatin on this promoting or inhibiting influence was also studied. The subsequent analysis focused on changes in the expression of genes associated with cancer progression. We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different "sensitizing ratio". Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance. On the other hand, CCL2 expression was associated with sensitizing effect. Significantly higher amounts of cisplatin were found in CAFs derived from patients who subsequently experienced a recurrence. In conclusion, our results showed that CAFs could promote and/or inhibit colony-forming capability and cisplatin resistance in HNSCC cells via paracrine effects and subsequent changes in gene expression of cancer-associated genes in cancer cells.

Links

GA18-03978S, research and development project

Name: Efekt buněčné tuhosti a mikroprostředí jako prediktor odpovědi na léčbu karcinomů hlavy a krku

Investor: Czech Science Foundation

MUNI/A/1246/2020, interní kód MU

Name: Kardiovaskulární systém: od iontového kanálu k celotělovému modelu (Acronym: KAVASYKAMO)

Investor: Masaryk University

MUNI/A/1698/2020, interní kód MU

Name: Od molekulární, buněčné a tkáňové k systémové patofyziologii vybraných komplexních nemocí (Acronym: ComplexPF)

Investor: Masaryk University

NV18-08-00229, research and development project

Name: Interakce buněk nádorového mikroprostředí jako nový nástroj predikce účinnosti terapie nádorů hlavy a krku

Investor: Ministry of Health of the CR

ROZV/28/LF25/2020, interní kód MU

Name: Odhaleni vlivu metabolické symbiózy a fibroblastů asociovaných s nádorem na invazívitu a migračnl potenciál HNSCC buněčných linii

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

PELTANOVÁ, Barbora, Markéta LIŠKOVÁ, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana HOLCOVÁ POLANSKÁ, Tomáš VACULOVIČ, David KALFERT, Marek GREGA, Jan PLZAK, Jan BETKA and Michal MASAŘÍK. Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts. International Journal of Molecular Sciences. Basel: MDPI, 2021, vol. 22, No 4, p. 1-13. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms22041912.

@article{1787377,
   author = {Peltanová, Barbora and Lišková, Markéta and Gumulec, Jaromír and Raudenská, Martina and Holcová Polanská, Hana and Vaculovič, Tomáš and Kalfert, David and Grega, Marek and Plzak, Jan and Betka, Jan and Masařík, Michal},
   article_location = {Basel},
   article_number = {4},
   doi = {http://dx.doi.org/10.3390/ijms22041912},
   keywords = {head and neck cancer; cancer-associated fibroblasts; cisplatin; treatment resistance; cancer recurrence; patient-derived cell cultures; coculture},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts},
   url = {https://www.mdpi.com/1422-0067/22/4/1912},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1787377
AU  - Peltanová, Barbora - Lišková, Markéta - Gumulec, Jaromír - Raudenská, Martina - Holcová Polanská, Hana - Vaculovič, Tomáš - Kalfert, David - Grega, Marek - Plzak, Jan - Betka, Jan - Masařík, Michal
PY  - 2021
TI  - Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 4
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 14220067
KW  - head and neck cancer
KW  - cancer-associated fibroblasts
KW  - cisplatin
KW  - treatment resistance
KW  - cancer recurrence
KW  - patient-derived cell cultures
KW  - coculture
UR  - https://www.mdpi.com/1422-0067/22/4/1912
N2  - Cancer-associated fibroblasts (CAFs) are one of the most abundant and critical components of the tumor stroma. CAFs can impact many important steps of cancerogenesis and may also influence treatment resistance. Some of these effects need the direct contact of CAFs and cancer cells, while some involve paracrine signals. In this study, we investigated the ability of head and neck squamous cell carcinomas (HNSCC) patient-derived CAFs to promote or inhibit the colony-forming ability of HNSCC cells. The effect of cisplatin on this promoting or inhibiting influence was also studied. The subsequent analysis focused on changes in the expression of genes associated with cancer progression. We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different "sensitizing ratio". Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance. On the other hand, CCL2 expression was associated with sensitizing effect. Significantly higher amounts of cisplatin were found in CAFs derived from patients who subsequently experienced a recurrence. In conclusion, our results showed that CAFs could promote and/or inhibit colony-forming capability and cisplatin resistance in HNSCC cells via paracrine effects and subsequent changes in gene expression of cancer-associated genes in cancer cells.
ER  -

PELTANOVÁ, Barbora, Markéta LIŠKOVÁ, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana HOLCOVÁ POLANSKÁ, Tomáš VACULOVIČ, David KALFERT, Marek GREGA, Jan PLZAK, Jan BETKA and Michal MASAŘÍK. Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2021, vol.~22, No~4, p.~1-13. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms22041912.

Detailed Information on Publication Record