CICCOCIOPPO, R., C. MENGOLI, E. BETTI, G. COMOLLI, I. CASSANITI, A. PIRALLA, Peter KRUŽLIAK, M. CAPRNDA, L. POZZI, G. R. CORAZZA, A. DI SABATINO and F. BALDANTI. Human Cytomegalovirus and Epstein-Barr virus specific immunity in patients with ulcerative colitis. Clinical and Experimental Medicine. Milan: SPRINGER-VERLAG ITALIA SRL, 2021, vol. 21, No 3, p. 379-388. ISSN 1591-8890. Available from: https://dx.doi.org/10.1007/s10238-021-00702-2.
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Basic information
Original name Human Cytomegalovirus and Epstein-Barr virus specific immunity in patients with ulcerative colitis
Authors CICCOCIOPPO, R., C. MENGOLI, E. BETTI, G. COMOLLI, I. CASSANITI, A. PIRALLA, Peter KRUŽLIAK (703 Slovakia, guarantor, belonging to the institution), M. CAPRNDA, L. POZZI, G. R. CORAZZA, A. DI SABATINO and F. BALDANTI.
Edition Clinical and Experimental Medicine, Milan, SPRINGER-VERLAG ITALIA SRL, 2021, 1591-8890.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher Italy
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.057
RIV identification code RIV/00216224:14110/21:00122152
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s10238-021-00702-2
UT WoS 000633271600001
Keywords in English Adaptive immunity; Epstein-Barr virus; Human Cytomegalovirus; Therapy; Ulcerative colitis
Tags 14110121, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 24/8/2021 09:54.
Abstract
Human Cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) are endowed with the ability of establishing lifelong latency in human hosts and reactivating in immunocompromised subjects, including patients suffering from ulcerative colitis (UC). We, therefore, aimed to investigate virus-specific immunity in UC patients. A cohort of 24 UC patients (14 responders and 10 refractory to therapy) and 26 control subjects was prospectively enrolled to undergo virus-specific serology (by ELISA assay) and assessment of both CD4(+) and CD8(+) virus-specific T-cell response (by interferon-gamma enzyme-linked immunospotanalysis). In parallel, mucosal viral load was determined by quantitative real-time PCR and the values were correlated with both clinical and endoscopic indexes of activity. For statistics, the t-test, Mann-Withney test, Fisher's exact test and Spearman rank correlation test were applied; p < 0.05 was considered significant. EBV-specific CD4(+) and CD8(+) T-cell responses were significantly lower in UC patients compared to controls (p < 0.0001 and p = 0.0006, respectively), whereas no difference was found for HCMV-specific T-cell response. When dividing the UC group according to response to therapy, both responders and refractory UC patients showed a deficient EBV-specific CD4(+) T-cell response with respect to controls (p < 0.04 and p = 0.0003, respectively). Moreover, both EBV and HCMV mucosal loads were significantly higher in refractory UC than in responders and controls (p = 0.007 and 0.003; and p = 0.02 and 0.001, respectively), and correlated with activity indexes. Steroid therapy seemed the main risk factor for triggering EBV colitis. Finally, no cases of IgM positivity were found in the study population. An impaired EBV-specific immunity was clearly evident in UC patients, mostly in those refractory to therapy. The ELISPOT assay may serve as new tool for quantifying and monitoring virus-specific T-cell immunity in UC.
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