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@article{1787540,
author = {Krtička, Milan and Plánka, Ladislav and Vojtová, Lucy and Nekuda, Vladimír and Stastny, Premysl and Sedlacek, Radek and Brinek, Adam and Kavkova, Michaela and Gopfert, Eduard and Hedvicakova, Vera and Rampichova, Michala and Křen, Leoš and Lišková, Květoslava and Ira, Daniel and Dorazilova, Jana and Suchy, Tomas and Zikmund, Tomas and Kaiser, Jozef and Starý, David and Faldyna, Martin and Trunec, Martin},
article_location = {BASEL},
article_number = {7},
doi = {http://dx.doi.org/10.3390/biomedicines9070733},
keywords = {micro-CT; biomechanics; histology; animal model; lumbar spinal fusion; tissue engineering; autograft; ceramic; collagen; FGF2},
language = {eng},
issn = {2227-9059},
journal = {BIOMEDICINES},
title = {Lumbar Interbody Fusion Conducted on a Porcine Model with a Bioresorbable Ceramic/Biopolymer Hybrid Implant Enriched with Hyperstable Fibroblast Growth Factor 2},
url = {https://www.mdpi.com/2227-9059/9/7/733},
volume = {9},
year = {2021}
}
TY - JOUR
ID - 1787540
AU - Krtička, Milan - Plánka, Ladislav - Vojtová, Lucy - Nekuda, Vladimír - Stastny, Premysl - Sedlacek, Radek - Brinek, Adam - Kavkova, Michaela - Gopfert, Eduard - Hedvicakova, Vera - Rampichova, Michala - Křen, Leoš - Lišková, Květoslava - Ira, Daniel - Dorazilova, Jana - Suchy, Tomas - Zikmund, Tomas - Kaiser, Jozef - Starý, David - Faldyna, Martin - Trunec, Martin
PY - 2021
TI - Lumbar Interbody Fusion Conducted on a Porcine Model with a Bioresorbable Ceramic/Biopolymer Hybrid Implant Enriched with Hyperstable Fibroblast Growth Factor 2
JF - BIOMEDICINES
VL - 9
IS - 7
SP - 1-25
EP - 1-25
PB - MDPI
SN - 22279059
KW - micro-CT
KW - biomechanics
KW - histology
KW - animal model
KW - lumbar spinal fusion
KW - tissue engineering
KW - autograft
KW - ceramic
KW - collagen
KW - FGF2
UR - https://www.mdpi.com/2227-9059/9/7/733
N2 - Many growth factors have been studied as additives accelerating lumbar fusion rates in different animal models. However, their low hydrolytic and thermal stability both in vitro and in vivo limits their workability and use. In the proposed work, a stabilized vasculogenic and prohealing fibroblast growth factor-2 (FGF2-STAB(R)) exhibiting a functional half-life in vitro at 37 degrees C more than 20 days was applied for lumbar fusion in combination with a bioresorbable scaffold on porcine models. An experimental animal study was designed to investigate the intervertebral fusion efficiency and safety of a bioresorbable ceramic/biopolymer hybrid implant enriched with FGF2-STAB(R) in comparison with a tricortical bone autograft used as a gold standard. Twenty-four experimental pigs underwent L2/3 discectomy with implantation of either the tricortical iliac crest bone autograft or the bioresorbable hybrid implant (BHI) followed by lateral intervertebral fixation. The quality of spinal fusion was assessed by micro-computed tomography (micro-CT), biomechanical testing, and histological examination at both 8 and 16 weeks after the surgery. While 8 weeks after implantation, micro-CT analysis demonstrated similar fusion quality in both groups, in contrast, spines with BHI involving inorganic hydroxyapatite and tricalcium phosphate along with organic collagen, oxidized cellulose, and FGF2- STAB(R) showed a significant increase in a fusion quality in comparison to the autograft group 16 weeks post-surgery (p = 0.023). Biomechanical testing revealed significantly higher stiffness of spines treated with the bioresorbable hybrid implant group compared to the autograft group (p < 0.05). Whilst histomorphological evaluation showed significant progression of new bone formation in the BHI group besides non-union and fibrocartilage tissue formed in the autograft group. Significant osteoinductive effects of BHI based on bioceramics, collagen, oxidized cellulose, and FGF2-STAB(R) could improve outcomes in spinal fusion surgery and bone tissue regeneration.
ER -
KRTIČKA, Milan, Ladislav PLÁNKA, Lucy VOJTOVÁ, Vladimír NEKUDA, Premysl STASTNY, Radek SEDLACEK, Adam BRINEK, Michaela KAVKOVA, Eduard GOPFERT, Vera HEDVICAKOVA, Michala RAMPICHOVA, Leoš KŘEN, Květoslava LIŠKOVÁ, Daniel IRA, Jana DORAZILOVA, Tomas SUCHY, Tomas ZIKMUND, Jozef KAISER, David STARÝ, Martin FALDYNA and Martin TRUNEC. Lumbar Interbody Fusion Conducted on a Porcine Model with a Bioresorbable Ceramic/Biopolymer Hybrid Implant Enriched with Hyperstable Fibroblast Growth Factor 2. \textit{BIOMEDICINES}. BASEL: MDPI, 2021, vol.~9, No~7, p.~1-25. ISSN~2227-9059. Available from: https://dx.doi.org/10.3390/biomedicines9070733.
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