Antibiotic Resistance in Wastewater and Its Impact on a Receiving River: A Case Study of WWTP Brno-Modřice, Czech Republic

BURIÁNKOVÁ, Iva, Peter KUCHTA, Anna MOLÍKOVÁ, Kateřina SOVOVÁ, David VÝRAVSKÝ, Martin RULÍK, David NOVÁK, Jan LOCHMAN and Monika VÍTĚZOVÁ. Antibiotic Resistance in Wastewater and Its Impact on a Receiving River: A Case Study of WWTP Brno-Modřice, Czech Republic. Water. MDPI, 2021, vol. 13, No 16, p. "2309", 18 pp. ISSN 2073-4441. Available from: https://dx.doi.org/10.3390/w13162309.

Basic information

• Original name: Antibiotic Resistance in Wastewater and Its Impact on a Receiving River: A Case Study of WWTP Brno-Modřice, Czech Republic
• Authors: BURIÁNKOVÁ, Iva (203 Czech Republic, belonging to the institution), Peter KUCHTA (703 Slovakia, belonging to the institution), Anna MOLÍKOVÁ (203 Czech Republic, belonging to the institution), Kateřina SOVOVÁ, David VÝRAVSKÝ, Martin RULÍK, David NOVÁK (203 Czech Republic, belonging to the institution), Jan LOCHMAN (203 Czech Republic, belonging to the institution) and Monika VÍTĚZOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Water, MDPI, 2021, 2073-4441.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.530
• RIV identification code: RIV/00216224:14310/21:00122194
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.3390/w13162309
• UT WoS: 000690243000001
• Keywords in English: antibiotic resistance; wastewater treatment plant; antibiotics; genes coding for antibiotic resistance; antibiotic resistant bacteria; qPCR; WWTPs efficiency
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Antibiotic resistance has become a global threat in which the anthropogenically influenced aquatic environment represents not only a reservoir for the spread of antibiotic resistant bacteria (ARB) among humans and animals but also an environment where resistance genes are introduced into natural microbial ecosystems. Wastewater is one of the sources of antibiotic resistance. The aim of this research was the evaluation of wastewater impact on the spread of antibiotic resistance in the water environment. In this study, qPCR was used to detect antibiotic resistance genes (ARGs)—blaCTX-M-15, blaCTX-M-32, ampC, blaTEM, sul1, tetM and mcr-1 and an integron detection primer (intl1). Detection of antibiotic resistant Escherichia coli was used as a complement to the observed qPCR results. Our results show that the process of wastewater treatment significantly reduces the abundances of ARGs and ARB. Nevertheless, treated wastewater affects the ARGs and ARB number in the receiving river.