Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)

J 2021

Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)

VOZÁROVÁ, Radka, Eliška MACKOVÁ, David VLK and Jana ŘEPKOVÁ

Basic information

Original name

Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)

Authors

VOZÁROVÁ, Radka (703 Slovakia, belonging to the institution), Eliška MACKOVÁ (203 Czech Republic, belonging to the institution), David VLK (203 Czech Republic, belonging to the institution) and Jana ŘEPKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plants, MDPI, 2021, 2223-7747

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.658

RIV identification code

RIV/00216224:14310/21:00122222

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/plants10091771

UT WoS

000701576700001

Keywords in English

5S rDNA; 26S rDNA; clover; fluorescent in situ hybridization; nucleotide polymorphism; genome structure

Abstract

V originále

The genus Trifolium L. is characterized by basic chromosome numbers 8, 7, 6, and 5. We conducted a genus-wide study of ribosomal DNA (rDNA) structure variability in diploids and polyploids to gain insight into evolutionary history. We used fluorescent in situ hybridization to newly investigate rDNA variation by number and position in 30 Trifolium species. Evolutionary history among species was examined using 85 available sequences of internal transcribed spacer 1 (ITS1) of 35S rDNA. In diploid species with ancestral basic chromosome number (x = 8), one pair of 5S and 26S rDNA in separate or adjacent positions on a pair of chromosomes was prevalent. Genomes of species with reduced basic chromosome numbers were characterized by increased number of signals determined on one pair of chromosomes or all chromosomes. Increased number of signals was observed also in diploids Trifolium alpestre and Trifolium microcephalum and in polyploids. Sequence alignment revealed ITS1 sequences with mostly single nucleotide polymorphisms, and ITS1 diversity was greater in diploids with reduced basic chromosome numbers compared to diploids with ancestral basic chromosome number (x = 8) and polyploids. Our results suggest the presence of one 5S rDNA site and one 26S rDNA site as an ancestral state.

Links

MUNI/A/1522/2020, interní kód MU

Name: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9 (Acronym: MBG9)

Investor: Masaryk University

Citovat

VOZÁROVÁ, Radka, Eliška MACKOVÁ, David VLK and Jana ŘEPKOVÁ. Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae). Plants. MDPI, 2021, vol. 10, No 9, p. "1771", 16 pp. ISSN 2223-7747. Available from: https://dx.doi.org/10.3390/plants10091771.

@article{1789338,
   author = {Vozárová, Radka and Macková, Eliška and Vlk, David and Řepková, Jana},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/plants10091771},
   keywords = {5S rDNA; 26S rDNA; clover; fluorescent in situ hybridization; nucleotide polymorphism; genome structure},
   language = {eng},
   issn = {2223-7747},
   journal = {Plants},
   title = {Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)},
   url = {https://doi.org/10.3390/plants10091771},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1789338
AU  - Vozárová, Radka - Macková, Eliška - Vlk, David - Řepková, Jana
PY  - 2021
TI  - Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)
JF  - Plants
VL  - 10
IS  - 9
SP  - "1771"
EP  - "1771"
PB  - MDPI
SN  - 22237747
KW  - 5S rDNA
KW  - 26S rDNA
KW  - clover
KW  - fluorescent in situ hybridization
KW  - nucleotide polymorphism
KW  - genome structure
UR  - https://doi.org/10.3390/plants10091771
N2  - The genus Trifolium L. is characterized by basic chromosome numbers 8, 7, 6, and 5. We conducted a genus-wide study of ribosomal DNA (rDNA) structure variability in diploids and polyploids to gain insight into evolutionary history. We used fluorescent in situ hybridization to newly investigate rDNA variation by number and position in 30 Trifolium species. Evolutionary history among species was examined using 85 available sequences of internal transcribed spacer 1 (ITS1) of 35S rDNA. In diploid species with ancestral basic chromosome number (x = 8), one pair of 5S and 26S rDNA in separate or adjacent positions on a pair of chromosomes was prevalent. Genomes of species with reduced basic chromosome numbers were characterized by increased number of signals determined on one pair of chromosomes or all chromosomes. Increased number of signals was observed also in diploids Trifolium alpestre and Trifolium microcephalum and in polyploids. Sequence alignment revealed ITS1 sequences with mostly single nucleotide polymorphisms, and ITS1 diversity was greater in diploids with reduced basic chromosome numbers compared to diploids with ancestral basic chromosome number (x = 8) and polyploids. Our results suggest the presence of one 5S rDNA site and one 26S rDNA site as an ancestral state.
ER  -

VOZÁROVÁ, Radka, Eliška MACKOVÁ, David VLK and Jana ŘEPKOVÁ. Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae). \textit{Plants}. MDPI, 2021, vol.~10, No~9, p.~''1771'', 16 pp. ISSN~2223-7747. Available from: https://dx.doi.org/10.3390/plants10091771.

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