Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)

VOZÁROVÁ, Radka, Eliška MACKOVÁ, David VLK and Jana ŘEPKOVÁ. Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae). Plants. MDPI, 2021, vol. 10, No 9, p. "1771", 16 pp. ISSN 2223-7747. Available from: https://dx.doi.org/10.3390/plants10091771.

Basic information

• Original name: Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)
• Authors: VOZÁROVÁ, Radka (703 Slovakia, belonging to the institution), Eliška MACKOVÁ (203 Czech Republic, belonging to the institution), David VLK (203 Czech Republic, belonging to the institution) and Jana ŘEPKOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Plants, MDPI, 2021, 2223-7747.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 4.658
• RIV identification code: RIV/00216224:14310/21:00122222
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.3390/plants10091771
• UT WoS: 000701576700001
• Keywords in English: 5S rDNA; 26S rDNA; clover; fluorescent in situ hybridization; nucleotide polymorphism; genome structure
• Tags: CF SAXS
• Tags: International impact, Reviewed

Abstract

The genus Trifolium L. is characterized by basic chromosome numbers 8, 7, 6, and 5. We conducted a genus-wide study of ribosomal DNA (rDNA) structure variability in diploids and polyploids to gain insight into evolutionary history. We used fluorescent in situ hybridization to newly investigate rDNA variation by number and position in 30 Trifolium species. Evolutionary history among species was examined using 85 available sequences of internal transcribed spacer 1 (ITS1) of 35S rDNA. In diploid species with ancestral basic chromosome number (x = 8), one pair of 5S and 26S rDNA in separate or adjacent positions on a pair of chromosomes was prevalent. Genomes of species with reduced basic chromosome numbers were characterized by increased number of signals determined on one pair of chromosomes or all chromosomes. Increased number of signals was observed also in diploids Trifolium alpestre and Trifolium microcephalum and in polyploids. Sequence alignment revealed ITS1 sequences with mostly single nucleotide polymorphisms, and ITS1 diversity was greater in diploids with reduced basic chromosome numbers compared to diploids with ancestral basic chromosome number (x = 8) and polyploids. Our results suggest the presence of one 5S rDNA site and one 26S rDNA site as an ancestral state.

Links

• MUNI/A/1522/2020, interní kód MU: Name: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9 (Acronym: MBG9)

Investor: Masaryk University