Molecular mechanisms of zooplanktonic toxicity in the okadaic
acid- producing dinoflagellate Prorocentrum lima

J 2021

Molecular mechanisms of zooplanktonic toxicity in the okadaic acid- producing dinoflagellate Prorocentrum lima

GONG, Yufeng, Keke ZHANG, Ningbo GENG, Minghuo WU, Xianliang YI et. al.

Basic information

Original name

Molecular mechanisms of zooplanktonic toxicity in the okadaic acid- producing dinoflagellate Prorocentrum lima

Authors

GONG, Yufeng (156 China), Keke ZHANG (156 China), Ningbo GENG (156 China), Minghuo WU (156 China), Xianliang YI (156 China), Renyan RY (156 China), Jonathan K. CHALLIS (124 Canada), Garry Paul CODLING (826 United Kingdom of Great Britain and Northern Ireland, guarantor, belonging to the institution), Elvis Genbo XU (156 China) and John Paul GIESY (840 United States of America)

Edition

Environmental Pollution, OXFORD, OXON, ENGLAND, ELSEVIER SCI LTD, 2021, 0269-7491

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.988

RIV identification code

RIV/00216224:14310/21:00122227

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.envpol.2021.116942

UT WoS

000647790400022

Keywords in English

Transcriptomic; Metabolomics; Toxic algal bloom; Zooplankton

Abstract

V originále

Prorocentrum lima is a dinoflagellate that forms hazardous blooms and produces okadaic acid (OA), leading to adverse environmental consequences associated with the declines of zooplankton pop-ulations. However, little is known about the toxic effects and molecular mechanisms of P. lima or OA on zooplankton. Here, their toxic effects were investigated using the brine shrimp Artemia salina. Acute exposure of A. salina to P. lima resulted in lethality at concentrations 10 0-fold lower than densities observed during blooms. The first comprehensive results from global transcriptomic and metabolomic analyses in A. salina showed up-regulated mRNA expression of antioxidant enzymes and reduced non-enzyme antioxidants, indicating general detoxification responses to oxidative stress after exposure to P. lima. The significantly up-regulated mRNA expression of proteasome, spliceosome, and ribosome, as well as the increased fatty acid oxidation and oxidative phosphorylation suggested the proteolysis of damaged proteins and induction of energy expenditure. Exposure to OA increased catabolism of chitin, which may further disrupt the molting and reproduction activities of A. salina. Our data shed new in-sights on the molecular responses and toxicity mechanisms of A. salina to P. lima or OA. The simple zooplankton model integrated with omic methods provides a sensitive assessment approach for studying hazardous algae.

Links

EF15_003/0000469, research and development project

Name: Cetocoen Plus

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

839243, interní kód MU

Name: Finding unknown endocrine disrupting compounds through target pull-down assay filtration, effect direct analysis and ultra-high resolution mass spectrometry for a comprehensive efficient workflow (Acronym: PullEd-MS)

Investor: European Union, MSCA Marie Skłodowska-Curie Actions (Excellent Science)

Citovat

GONG, Yufeng, Keke ZHANG, Ningbo GENG, Minghuo WU, Xianliang YI, Renyan RY, Jonathan K. CHALLIS, Garry Paul CODLING, Elvis Genbo XU and John Paul GIESY. Molecular mechanisms of zooplanktonic toxicity in the okadaic acid- producing dinoflagellate Prorocentrum lima. Environmental Pollution. OXFORD, OXON, ENGLAND: ELSEVIER SCI LTD, 2021, vol. 279, June 2021, p. 1-10. ISSN 0269-7491. Available from: https://dx.doi.org/10.1016/j.envpol.2021.116942.

@article{1789403,
   author = {Gong, Yufeng and Zhang, Keke and Geng, Ningbo and Wu, Minghuo and Yi, Xianliang and RY, Renyan and Challis, Jonathan K. and Codling, Garry Paul and Xu, Elvis Genbo and Giesy, John Paul},
   article_location = {OXFORD, OXON, ENGLAND},
   article_number = {June 2021},
   doi = {http://dx.doi.org/10.1016/j.envpol.2021.116942},
   keywords = {Transcriptomic; Metabolomics; Toxic algal bloom; Zooplankton},
   language = {eng},
   issn = {0269-7491},
   journal = {Environmental Pollution},
   title = {Molecular mechanisms of zooplanktonic toxicity in the okadaic acid- producing dinoflagellate Prorocentrum lima},
   url = {https://www.sciencedirect.com/science/article/pii/S0269749121005248?via%3Dihub},
   volume = {279},
   year = {2021}
}

TY  - JOUR
ID  - 1789403
AU  - Gong, Yufeng - Zhang, Keke - Geng, Ningbo - Wu, Minghuo - Yi, Xianliang - RY, Renyan - Challis, Jonathan K. - Codling, Garry Paul - Xu, Elvis Genbo - Giesy, John Paul
PY  - 2021
TI  - Molecular mechanisms of zooplanktonic toxicity in the okadaic acid- producing dinoflagellate Prorocentrum lima
JF  - Environmental Pollution
VL  - 279
IS  - June 2021
SP  - 1-10
EP  - 1-10
PB  - ELSEVIER SCI LTD
SN  - 02697491
KW  - Transcriptomic
KW  - Metabolomics
KW  - Toxic algal bloom
KW  - Zooplankton
UR  - https://www.sciencedirect.com/science/article/pii/S0269749121005248?via%3Dihub
N2  - Prorocentrum lima is a dinoflagellate that forms hazardous blooms and produces okadaic acid (OA), leading to adverse environmental consequences associated with the declines of zooplankton pop-ulations. However, little is known about the toxic effects and molecular mechanisms of P. lima or OA on zooplankton. Here, their toxic effects were investigated using the brine shrimp Artemia salina. Acute exposure of A. salina to P. lima resulted in lethality at concentrations 10 0-fold lower than densities observed during blooms. The first comprehensive results from global transcriptomic and metabolomic analyses in A. salina showed up-regulated mRNA expression of antioxidant enzymes and reduced non-enzyme antioxidants, indicating general detoxification responses to oxidative stress after exposure to P. lima. The significantly up-regulated mRNA expression of proteasome, spliceosome, and ribosome, as well as the increased fatty acid oxidation and oxidative phosphorylation suggested the proteolysis of damaged proteins and induction of energy expenditure. Exposure to OA increased catabolism of chitin, which may further disrupt the molting and reproduction activities of A. salina. Our data shed new in-sights on the molecular responses and toxicity mechanisms of A. salina to P. lima or OA. The simple zooplankton model integrated with omic methods provides a sensitive assessment approach for studying hazardous algae.
ER  -

GONG, Yufeng, Keke ZHANG, Ningbo GENG, Minghuo WU, Xianliang YI, Renyan RY, Jonathan K. CHALLIS, Garry Paul CODLING, Elvis Genbo XU and John Paul GIESY. Molecular mechanisms of zooplanktonic toxicity in the okadaic acid- producing dinoflagellate Prorocentrum lima. \textit{Environmental Pollution}. OXFORD, OXON, ENGLAND: ELSEVIER SCI LTD, 2021, vol.~279, June 2021, p.~1-10. ISSN~0269-7491. Available from: https://dx.doi.org/10.1016/j.envpol.2021.116942.

Detailed Information on Publication Record