| KUBÍČKOVÁ, Barbara, Joerg A. SCHENK, Franziska RAMM, Kornelija MARKUSKIENE, Jochen REETZ, Paul DREMSEK, Paulius Lukas TAMOSIUNAS, Laima CEPULYTE, Hoai Anh TRINH, Johannes SCHOLZ, Henry MEMCZAK, Marc HOVESTAEDT, Rene RYLL, Rasa PETRAITYTE-BURNEIKIENE, Victor M. CORMAN, Anika ANDERSSON, Dietmar BECHER, Martin H. GROSCHUP, Stefan KUBICK, Frank SELLRIE, Reimar JOHNE and Rainer G. ULRICH. A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen. Applied Microbiology and Biotechnology. NEW YORK: SPRINGER, 2021, vol. 105, No 12, p. 4957-4973. ISSN 0175-7598. Available from: https://dx.doi.org/10.1007/s00253-021-11342-7. |
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@article{1789777,
author = {Kubíčková, Barbara and Schenk, Joerg A. and Ramm, Franziska and Markuskiene, Kornelija and Reetz, Jochen and Dremsek, Paul and Tamosiunas, Paulius Lukas and Cepulyte, Laima and Trinh, Hoai Anh and Scholz, Johannes and Memczak, Henry and Hovestaedt, Marc and Ryll, Rene and PetraityteandBurneikiene, Rasa and Corman, Victor M. and Andersson, Anika and Becher, Dietmar and Groschup, Martin H. and Kubick, Stefan and Sellrie, Frank and Johne, Reimar and Ulrich, Rainer G.},
article_location = {NEW YORK},
article_number = {12},
doi = {http://dx.doi.org/10.1007/s00253-021-11342-7},
keywords = {Hepatitis E virus; HEV-1; HEV-2; HEV-3; HEV-4; HEV-7; ratHEV; batHEV; cvHEV; Monoclonal antibody; Cross-reactivity; Cell-free synthesis},
language = {eng},
issn = {0175-7598},
journal = {Applied Microbiology and Biotechnology},
title = {A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen},
url = {https://link.springer.com/article/10.1007%2Fs00253-021-11342-7},
volume = {105},
year = {2021}
}
TY - JOUR
ID - 1789777
AU - Kubíčková, Barbara - Schenk, Joerg A. - Ramm, Franziska - Markuskiene, Kornelija - Reetz, Jochen - Dremsek, Paul - Tamosiunas, Paulius Lukas - Cepulyte, Laima - Trinh, Hoai Anh - Scholz, Johannes - Memczak, Henry - Hovestaedt, Marc - Ryll, Rene - Petraityte-Burneikiene, Rasa - Corman, Victor M. - Andersson, Anika - Becher, Dietmar - Groschup, Martin H. - Kubick, Stefan - Sellrie, Frank - Johne, Reimar - Ulrich, Rainer G.
PY - 2021
TI - A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen
JF - Applied Microbiology and Biotechnology
VL - 105
IS - 12
SP - 4957-4973
EP - 4957-4973
PB - SPRINGER
SN - 01757598
KW - Hepatitis E virus
KW - HEV-1
KW - HEV-2
KW - HEV-3
KW - HEV-4
KW - HEV-7
KW - ratHEV
KW - batHEV
KW - cvHEV
KW - Monoclonal antibody
KW - Cross-reactivity
KW - Cell-free synthesis
UR - https://link.springer.com/article/10.1007%2Fs00253-021-11342-7
N2 - To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)-specific monoclonal antibody (mAb), the Escherichia coli-expressed carboxy-terminal part of its capsid protein was used to immunise BALB/c mice. The immunisation resulted in the induction of HEV-specific antibodies of high titre. The mAb G117-AA4 of IgG1 isotype was obtained showing a strong reactivity with the homologous E. coli, but also yeast-expressed capsid protein of HEV-3. The mAb strongly cross-reacted with ratHEV capsid protein derivatives produced in both expression systems and weaker with an E. coli-expressed batHEV capsid protein fragment. In addition, the mAb reacted with capsid protein derivatives of genotypes HEV-2 and HEV-4 and common vole hepatitis E virus (cvHEV), produced by the cell-free synthesis in Chinese hamster ovary (CHO) and Spodoptera frugiperda (Sf21) cell lysates. Western blot and line blot reactivity of the mAb with capsid protein derivatives of HEV-1 to HEV-4, cvHEV, ratHEV and batHEV suggested a linear epitope. Use of truncated derivatives of ratHEV capsid protein in ELISA, Western blot, and a Pepscan analysis allowed to map the epitope within a partially surface-exposed region with the amino acid sequence LYTSV. The mAb was also shown to bind to human patient-derived HEV-3 from infected cell culture and to hare HEV-3 and camel HEV-7 capsid proteins from transfected cells by immunofluorescence assay. The novel mAb may serve as a useful tool for further investigations on the pathogenesis of HEV infections and might be used for diagnostic purposes.
ER -
KUBÍČKOVÁ, Barbara, Joerg A. SCHENK, Franziska RAMM, Kornelija MARKUSKIENE, Jochen REETZ, Paul DREMSEK, Paulius Lukas TAMOSIUNAS, Laima CEPULYTE, Hoai Anh TRINH, Johannes SCHOLZ, Henry MEMCZAK, Marc HOVESTAEDT, Rene RYLL, Rasa PETRAITYTE-BURNEIKIENE, Victor M. CORMAN, Anika ANDERSSON, Dietmar BECHER, Martin H. GROSCHUP, Stefan KUBICK, Frank SELLRIE, Reimar JOHNE and Rainer G. ULRICH. A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen. \textit{Applied Microbiology and Biotechnology}. NEW YORK: SPRINGER, 2021, vol.~105, No~12, p.~4957-4973. ISSN~0175-7598. Available from: https://dx.doi.org/10.1007/s00253-021-11342-7.
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