A broadly cross-reactive monoclonal antibody against hepatitis
E virus capsid antigen

J 2021

A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen

KUBÍČKOVÁ, Barbara, Joerg A. SCHENK, Franziska RAMM, Kornelija MARKUSKIENE, Jochen REETZ et. al.

Basic information

Original name

A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen

Authors

KUBÍČKOVÁ, Barbara (276 Germany, guarantor, belonging to the institution), Joerg A. SCHENK (276 Germany), Franziska RAMM (276 Germany), Kornelija MARKUSKIENE (276 Germany), Jochen REETZ (276 Germany), Paul DREMSEK (276 Germany), Paulius Lukas TAMOSIUNAS (440 Lithuania), Laima CEPULYTE (440 Lithuania), Hoai Anh TRINH (276 Germany), Johannes SCHOLZ (276 Germany), Henry MEMCZAK (276 Germany), Marc HOVESTAEDT (276 Germany), Rene RYLL (276 Germany), Rasa PETRAITYTE-BURNEIKIENE (440 Lithuania), Victor M. CORMAN (276 Germany), Anika ANDERSSON (276 Germany), Dietmar BECHER (276 Germany), Martin H. GROSCHUP (276 Germany), Stefan KUBICK (276 Germany), Frank SELLRIE (276 Germany), Reimar JOHNE (276 Germany) and Rainer G. ULRICH (276 Germany)

Edition

Applied Microbiology and Biotechnology, NEW YORK, SPRINGER, 2021, 0175-7598

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30405 Medical biotechnology related ethics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.560

RIV identification code

RIV/00216224:14310/21:00122250

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00253-021-11342-7

UT WoS

000661794700001

Keywords in English

Hepatitis E virus; HEV-1; HEV-2; HEV-3; HEV-4; HEV-7; ratHEV; batHEV; cvHEV; Monoclonal antibody; Cross-reactivity; Cell-free synthesis

Abstract

V originále

To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)-specific monoclonal antibody (mAb), the Escherichia coli-expressed carboxy-terminal part of its capsid protein was used to immunise BALB/c mice. The immunisation resulted in the induction of HEV-specific antibodies of high titre. The mAb G117-AA4 of IgG1 isotype was obtained showing a strong reactivity with the homologous E. coli, but also yeast-expressed capsid protein of HEV-3. The mAb strongly cross-reacted with ratHEV capsid protein derivatives produced in both expression systems and weaker with an E. coli-expressed batHEV capsid protein fragment. In addition, the mAb reacted with capsid protein derivatives of genotypes HEV-2 and HEV-4 and common vole hepatitis E virus (cvHEV), produced by the cell-free synthesis in Chinese hamster ovary (CHO) and Spodoptera frugiperda (Sf21) cell lysates. Western blot and line blot reactivity of the mAb with capsid protein derivatives of HEV-1 to HEV-4, cvHEV, ratHEV and batHEV suggested a linear epitope. Use of truncated derivatives of ratHEV capsid protein in ELISA, Western blot, and a Pepscan analysis allowed to map the epitope within a partially surface-exposed region with the amino acid sequence LYTSV. The mAb was also shown to bind to human patient-derived HEV-3 from infected cell culture and to hare HEV-3 and camel HEV-7 capsid proteins from transfected cells by immunofluorescence assay. The novel mAb may serve as a useful tool for further investigations on the pathogenesis of HEV infections and might be used for diagnostic purposes.

Citovat

KUBÍČKOVÁ, Barbara, Joerg A. SCHENK, Franziska RAMM, Kornelija MARKUSKIENE, Jochen REETZ, Paul DREMSEK, Paulius Lukas TAMOSIUNAS, Laima CEPULYTE, Hoai Anh TRINH, Johannes SCHOLZ, Henry MEMCZAK, Marc HOVESTAEDT, Rene RYLL, Rasa PETRAITYTE-BURNEIKIENE, Victor M. CORMAN, Anika ANDERSSON, Dietmar BECHER, Martin H. GROSCHUP, Stefan KUBICK, Frank SELLRIE, Reimar JOHNE and Rainer G. ULRICH. A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen. Applied Microbiology and Biotechnology. NEW YORK: SPRINGER, 2021, vol. 105, No 12, p. 4957-4973. ISSN 0175-7598. Available from: https://dx.doi.org/10.1007/s00253-021-11342-7.

@article{1789777,
   author = {Kubíčková, Barbara and Schenk, Joerg A. and Ramm, Franziska and Markuskiene, Kornelija and Reetz, Jochen and Dremsek, Paul and Tamosiunas, Paulius Lukas and Cepulyte, Laima and Trinh, Hoai Anh and Scholz, Johannes and Memczak, Henry and Hovestaedt, Marc and Ryll, Rene and PetraityteandBurneikiene, Rasa and Corman, Victor M. and Andersson, Anika and Becher, Dietmar and Groschup, Martin H. and Kubick, Stefan and Sellrie, Frank and Johne, Reimar and Ulrich, Rainer G.},
   article_location = {NEW YORK},
   article_number = {12},
   doi = {http://dx.doi.org/10.1007/s00253-021-11342-7},
   keywords = {Hepatitis E virus; HEV-1; HEV-2; HEV-3; HEV-4; HEV-7; ratHEV; batHEV; cvHEV; Monoclonal antibody; Cross-reactivity; Cell-free synthesis},
   language = {eng},
   issn = {0175-7598},
   journal = {Applied Microbiology and Biotechnology},
   title = {A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen},
   url = {https://link.springer.com/article/10.1007%2Fs00253-021-11342-7},
   volume = {105},
   year = {2021}
}

TY  - JOUR
ID  - 1789777
AU  - Kubíčková, Barbara - Schenk, Joerg A. - Ramm, Franziska - Markuskiene, Kornelija - Reetz, Jochen - Dremsek, Paul - Tamosiunas, Paulius Lukas - Cepulyte, Laima - Trinh, Hoai Anh - Scholz, Johannes - Memczak, Henry - Hovestaedt, Marc - Ryll, Rene - Petraityte-Burneikiene, Rasa - Corman, Victor M. - Andersson, Anika - Becher, Dietmar - Groschup, Martin H. - Kubick, Stefan - Sellrie, Frank - Johne, Reimar - Ulrich, Rainer G.
PY  - 2021
TI  - A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen
JF  - Applied Microbiology and Biotechnology
VL  - 105
IS  - 12
SP  - 4957-4973
EP  - 4957-4973
PB  - SPRINGER
SN  - 01757598
KW  - Hepatitis E virus
KW  - HEV-1
KW  - HEV-2
KW  - HEV-3
KW  - HEV-4
KW  - HEV-7
KW  - ratHEV
KW  - batHEV
KW  - cvHEV
KW  - Monoclonal antibody
KW  - Cross-reactivity
KW  - Cell-free synthesis
UR  - https://link.springer.com/article/10.1007%2Fs00253-021-11342-7
N2  - To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)-specific monoclonal antibody (mAb), the Escherichia coli-expressed carboxy-terminal part of its capsid protein was used to immunise BALB/c mice. The immunisation resulted in the induction of HEV-specific antibodies of high titre. The mAb G117-AA4 of IgG1 isotype was obtained showing a strong reactivity with the homologous E. coli, but also yeast-expressed capsid protein of HEV-3. The mAb strongly cross-reacted with ratHEV capsid protein derivatives produced in both expression systems and weaker with an E. coli-expressed batHEV capsid protein fragment. In addition, the mAb reacted with capsid protein derivatives of genotypes HEV-2 and HEV-4 and common vole hepatitis E virus (cvHEV), produced by the cell-free synthesis in Chinese hamster ovary (CHO) and Spodoptera frugiperda (Sf21) cell lysates. Western blot and line blot reactivity of the mAb with capsid protein derivatives of HEV-1 to HEV-4, cvHEV, ratHEV and batHEV suggested a linear epitope. Use of truncated derivatives of ratHEV capsid protein in ELISA, Western blot, and a Pepscan analysis allowed to map the epitope within a partially surface-exposed region with the amino acid sequence LYTSV. The mAb was also shown to bind to human patient-derived HEV-3 from infected cell culture and to hare HEV-3 and camel HEV-7 capsid proteins from transfected cells by immunofluorescence assay. The novel mAb may serve as a useful tool for further investigations on the pathogenesis of HEV infections and might be used for diagnostic purposes.
ER  -

KUBÍČKOVÁ, Barbara, Joerg A. SCHENK, Franziska RAMM, Kornelija MARKUSKIENE, Jochen REETZ, Paul DREMSEK, Paulius Lukas TAMOSIUNAS, Laima CEPULYTE, Hoai Anh TRINH, Johannes SCHOLZ, Henry MEMCZAK, Marc HOVESTAEDT, Rene RYLL, Rasa PETRAITYTE-BURNEIKIENE, Victor M. CORMAN, Anika ANDERSSON, Dietmar BECHER, Martin H. GROSCHUP, Stefan KUBICK, Frank SELLRIE, Reimar JOHNE and Rainer G. ULRICH. A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen. \textit{Applied Microbiology and Biotechnology}. NEW YORK: SPRINGER, 2021, vol.~105, No~12, p.~4957-4973. ISSN~0175-7598. Available from: https://dx.doi.org/10.1007/s00253-021-11342-7.

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