Universal two-point interaction of mediator KIX with 9aaTAD
activation domains

J 2021

Universal two-point interaction of mediator KIX with 9aaTAD activation domains

HOFROVÁ, Alena, Petr LOUŠA, Monika KUBÍČKOVÁ, Jozef HRITZ, Tomáš OTAŠEVIČ et. al.

Basic information

Original name

Universal two-point interaction of mediator KIX with 9aaTAD activation domains

Authors

HOFROVÁ, Alena (203 Czech Republic, belonging to the institution), Petr LOUŠA (203 Czech Republic, belonging to the institution), Monika KUBÍČKOVÁ (203 Czech Republic, belonging to the institution), Jozef HRITZ (703 Slovakia, belonging to the institution), Tomáš OTAŠEVIČ (203 Czech Republic, belonging to the institution), Martin REPKO (203 Czech Republic, belonging to the institution), Andrea KNIGHT (203 Czech Republic, belonging to the institution) and Martin PISKÁČEK (40 Austria, belonging to the institution)

Edition

Journal of cellular biochemistry, Hoboken (USA), WILEY-BLACKWELL, 2021, 0730-2312

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.480

RIV identification code

RIV/00216224:14110/21:00120140

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/jcb.30075

UT WoS

000669605600001

Keywords in English

9aaTAD; activation domain; E2A; KIX; MLL; p53

Abstract

V originále

The nine-amino-acid activation domain (9aaTAD) is defined by a short amino acid pattern including two hydrophobic regions (positions p3-4 and p6-7). The KIX domain of mediator transcription CBP interacts with the 9aaTAD domains of transcription factors MLL, E2A, NF-kB, and p53. In this study, we analyzed the 9aaTADs-KIX interactions by nuclear magnetic resonance. The positions of three KIX helixes alpha 1-alpha 2-alpha 3 are influenced by sterically-associated hydrophobic I611, L628, and I660 residues that are exposed to solvent. The positions of two rigid KIX helixes alpha 1 and alpha 2 generate conditions for structural folding in the flexible KIX-L12-G2 regions localized between them. The three KIX I611, L628, and I660 residues interact with two 9aaTAD hydrophobic residues in positions p3 and p4 and together build a hydrophobic core of five residues (5R). Numerous residues in 9aaTAD position p3 and p4 could provide this interaction. Following binding of the 9aaTAD to KIX, the hydrophobic I611, L628, and I660 residues are no longer exposed to solvent and their position changes inside the hydrophobic core together with position of KIX alpha 1-alpha 2-alpha 3 helixes. The new positions of the KIX helixes alpha 1 and alpha 2 allow the KIX-L12-G2 enhanced formation. The second hydrophobic region of the 9aaTAD (positions p6 and p7) provides strong binding with the KIX-L12-G2 region. Similarly, multiple residues in 9aaTAD position p6 and p7 could provide this interaction. In conclusion, both 9aaTAD regions p3, p4 and p6, p7 provide co-operative and highly universal binding to mediator KIX. The hydrophobic core 5R formation allows new positions of the rigid KIX alpha-helixes and enables the enhanced formation of the KIX-L12-G2 region. This contributes to free energy and is the key for the KIX-9aaTAD binding. Therefore, the 9aaTAD-KIX interactions do not operate under the rigid key-and-lock mechanism what explains the 9aaTAD natural variability.

Links

LTAUSA18168, research and development project

Name: Selektivní NMR značení jako nástroj pro charakterizaci proteinových komplexů zapojených do neurodegenerativních onemocnění

Investor: Ministry of Education, Youth and Sports of the CR, Selective NMR labelling as a tool for characterization of protein complexes involved in neurodegenerative diseases., INTER-ACTION

NV19-05-00410, research and development project

Name: Úloha cytotoxických gamma-delta T buněk na terapeutické rezistenci a recidivě Glioblastoma Multiforme

Investor: Ministry of Health of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

HOFROVÁ, Alena, Petr LOUŠA, Monika KUBÍČKOVÁ, Jozef HRITZ, Tomáš OTAŠEVIČ, Martin REPKO, Andrea KNIGHT and Martin PISKÁČEK. Universal two-point interaction of mediator KIX with 9aaTAD activation domains. Journal of cellular biochemistry. Hoboken (USA): WILEY-BLACKWELL, 2021, vol. 122, No 10, p. 1544-1555. ISSN 0730-2312. Available from: https://dx.doi.org/10.1002/jcb.30075.

@article{1790619,
   author = {Hofrová, Alena and Louša, Petr and Kubíčková, Monika and Hritz, Jozef and Otaševič, Tomáš and Repko, Martin and Knight, Andrea and Piskáček, Martin},
   article_location = {Hoboken (USA)},
   article_number = {10},
   doi = {http://dx.doi.org/10.1002/jcb.30075},
   keywords = {9aaTAD; activation domain; E2A; KIX; MLL; p53},
   language = {eng},
   issn = {0730-2312},
   journal = {Journal of cellular biochemistry},
   title = {Universal two-point interaction of mediator KIX with 9aaTAD activation domains},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/jcb.30075},
   volume = {122},
   year = {2021}
}

TY  - JOUR
ID  - 1790619
AU  - Hofrová, Alena - Louša, Petr - Kubíčková, Monika - Hritz, Jozef - Otaševič, Tomáš - Repko, Martin - Knight, Andrea - Piskáček, Martin
PY  - 2021
TI  - Universal two-point interaction of mediator KIX with 9aaTAD activation domains
JF  - Journal of cellular biochemistry
VL  - 122
IS  - 10
SP  - 1544-1555
EP  - 1544-1555
PB  - WILEY-BLACKWELL
SN  - 07302312
KW  - 9aaTAD
KW  - activation domain
KW  - E2A
KW  - KIX
KW  - MLL
KW  - p53
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jcb.30075
N2  - The nine-amino-acid activation domain (9aaTAD) is defined by a short amino acid pattern including two hydrophobic regions (positions p3-4 and p6-7). The KIX domain of mediator transcription CBP interacts with the 9aaTAD domains of transcription factors MLL, E2A, NF-kB, and p53. In this study, we analyzed the 9aaTADs-KIX interactions by nuclear magnetic resonance. The positions of three KIX helixes alpha 1-alpha 2-alpha 3 are influenced by sterically-associated hydrophobic I611, L628, and I660 residues that are exposed to solvent. The positions of two rigid KIX helixes alpha 1 and alpha 2 generate conditions for structural folding in the flexible KIX-L12-G2 regions localized between them. The three KIX I611, L628, and I660 residues interact with two 9aaTAD hydrophobic residues in positions p3 and p4 and together build a hydrophobic core of five residues (5R). Numerous residues in 9aaTAD position p3 and p4 could provide this interaction. Following binding of the 9aaTAD to KIX, the hydrophobic I611, L628, and I660 residues are no longer exposed to solvent and their position changes inside the hydrophobic core together with position of KIX alpha 1-alpha 2-alpha 3 helixes. The new positions of the KIX helixes alpha 1 and alpha 2 allow the KIX-L12-G2 enhanced formation. The second hydrophobic region of the 9aaTAD (positions p6 and p7) provides strong binding with the KIX-L12-G2 region. Similarly, multiple residues in 9aaTAD position p6 and p7 could provide this interaction. In conclusion, both 9aaTAD regions p3, p4 and p6, p7 provide co-operative and highly universal binding to mediator KIX. The hydrophobic core 5R formation allows new positions of the rigid KIX alpha-helixes and enables the enhanced formation of the KIX-L12-G2 region. This contributes to free energy and is the key for the KIX-9aaTAD binding. Therefore, the 9aaTAD-KIX interactions do not operate under the rigid key-and-lock mechanism what explains the 9aaTAD natural variability.
ER  -

HOFROVÁ, Alena, Petr LOUŠA, Monika KUBÍČKOVÁ, Jozef HRITZ, Tomáš OTAŠEVIČ, Martin REPKO, Andrea KNIGHT and Martin PISKÁČEK. Universal two-point interaction of mediator KIX with 9aaTAD activation domains. \textit{Journal of cellular biochemistry}. Hoboken (USA): WILEY-BLACKWELL, 2021, vol.~122, No~10, p.~1544-1555. ISSN~0730-2312. Available from: https://dx.doi.org/10.1002/jcb.30075.

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