Detailed Information on Publication Record
2021
Sensitive determination of tetracycline antibiotics by liquid chromatography with fluorescence and mass spectrometric detection
KUBINYIOVÁ, Lenka, Ondřej PEŠ, Josef KUČERA, Jan JUŘICA, Ondřej ZENDULKA et. al.Basic information
Original name
Sensitive determination of tetracycline antibiotics by liquid chromatography with fluorescence and mass spectrometric detection
Authors
KUBINYIOVÁ, Lenka (203 Czech Republic, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Josef KUČERA (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution), Ondřej ZENDULKA (203 Czech Republic, belonging to the institution), Pavel ŠMAK (203 Czech Republic, belonging to the institution) and Petr TÁBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)
Edition
22. ročník Školy hmotnostní spektrometrie, 2021
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10609 Biochemical research methods
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14110/21:00122331
Organization unit
Faculty of Medicine
ISBN
978-80-88195-25-2
Keywords in English
fluorescence; mass spectrometry; heavy water; tetracyclines
Změněno: 14/9/2021 10:38, doc. Mgr. Ondřej Peš, Ph.D.
Abstract
V originále
Tetracycline compounds present a broad spectrum of antibiotics widely used in medicine. Since their discovery they have been used in the treatment of human diseases as well as in veterinary applications. However, their overuse in food-producing animals may express an adverse effect on human health and even induce selective resistance. Selected antibiotics from the class of tetracyclines have been analyzed using liquid chromatography with fluorescence detection (LC-FLU) and LC with mass spectrometric detection (LC-MS) on a core-shell C18 column. As majority systems nowadays rely on reversed-phase columns with water being the main component of the mobile phase, a simple replacement of H 2 O with D 2 O in the mobile phase enhanced the sensitivity for selected compounds by 10–200%. MS was used to unambiguously identify the separated compounds and to locate the labile hydrogen sites. Another way to increase the fluorescence intensity lied in production of complex compounds of the tetracycline molecules and Mg 2+ ions. Such simple yet effective approaches improved limits of detection and sensitivity and may be selectively exploited (on demand) in the LC-FLU analysis of these compounds.
Links
| MUNI/A/1192/2020, interní kód MU |
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| MUNI/A/1249/2020, interní kód MU |
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| MUNI/A/1266/2020, interní kód MU |
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