Do foodborne polyethylene microparticles affect the health of
rainbow trout (Oncorhynchus mykiss)?

J 2021

Do foodborne polyethylene microparticles affect the health of rainbow trout (Oncorhynchus mykiss)?

HODKOVICOVA, N., A. HOLLEROVA, H. CALOUDOVA, J. BLAHOVA, Aleš FRANC et. al.

Basic information

Original name

Do foodborne polyethylene microparticles affect the health of rainbow trout (Oncorhynchus mykiss)?

Authors

HODKOVICOVA, N. (guarantor), A. HOLLEROVA, H. CALOUDOVA, J. BLAHOVA, Aleš FRANC (203 Czech Republic, belonging to the institution), Michaela GARAJOVÁ (703 Slovakia, belonging to the institution), J. LENZ, F. TICHY, M. FALDYNA, P. KULICH, J. MARES, R. MACHAT, V. ENEVOVA and Z. SVOBODOVA

Edition

Science of the Total Environment, Amsterdam, Elsevier Science, 2021, 0048-9697

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.753

RIV identification code

RIV/00216224:14160/21:00122347

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.1016/j.scitotenv.2021.148490

UT WoS

000691602900011

Keywords in English

Salmonidae; Antioxidant capacity; Plastic; Immunity; Toxicology; Electron microscopy

Abstract

V originále

Due to the fact that plastic pollution is a global environmental problem of modern age, studies on the impact of these synthetic materials on aquatic, and especially fish organisms, are an important part of the ecosystem and human nutrition. In our study, the toxicity of pristine polyethylene (PE) microparticles (approx. 50 mu m) on rainbow trout (Oncorhynchus mykiss) was tested in three different dietary concentrations - 0.5%, 2% and 5%. After six weeks of exposure, various health indices were evaluated. Electron microscopy of the intestine revealed the disintegration of PE particles to <5 mu m in size, and thus we concluded that microplastics are able to reach tissues. The haematological profile revealed changes in total red blood cells count and haematocrit (5% PE) which could be associated with spleen congestion observed histologically. The marker of lipid peroxidation was increased in gills suggesting the disruption of balance in antioxidant enzymes capacity and histopathological imaging revealed inflammation in higher PE concentrations. In addition, ammonia was decreased and calcium elevated in biochemical profile, confirming the gill damage. Electron microscopy of the gills showed lesions of lamellae and visible rings around the mucinous cell opening indicating their higher activity. Another injured was the liver tissue, as confirmed by hepatodystrophies and increased expression of pro-inflammatory genes in 2% PE. Impaired innate immunity was confirmed by an increased presence of mucinous cells and a decrease in leukocytes. Kidney damage manifested itself by higher expression of pro-inflammatory cytokines and histopathology. The damage in gills, liver and kidney together correlated with the increased antioxidant capacity of plasma. In conclusion, PE microparticles are able to affect health indices of O. mykiss. The potential problem for aquatic ecosystems and even human consumption should be considered. (c) 2021 Elsevier B.V. All rights reserved.

Citovat

HODKOVICOVA, N., A. HOLLEROVA, H. CALOUDOVA, J. BLAHOVA, Aleš FRANC, Michaela GARAJOVÁ, J. LENZ, F. TICHY, M. FALDYNA, P. KULICH, J. MARES, R. MACHAT, V. ENEVOVA and Z. SVOBODOVA. Do foodborne polyethylene microparticles affect the health of rainbow trout (Oncorhynchus mykiss)? Science of the Total Environment. Amsterdam: Elsevier Science, 2021, vol. 793, No 148490, p. 1-14. ISSN 0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2021.148490.

@article{1792157,
   author = {Hodkovicova, N. and Hollerova, A. and Caloudova, H. and Blahova, J. and Franc, Aleš and Garajová, Michaela and Lenz, J. and Tichy, F. and Faldyna, M. and Kulich, P. and Mares, J. and Machat, R. and Enevova, V. and Svobodova, Z.},
   article_location = {Amsterdam},
   article_number = {148490},
   doi = {http://dx.doi.org/10.1016/j.scitotenv.2021.148490},
   keywords = {Salmonidae; Antioxidant capacity; Plastic; Immunity; Toxicology; Electron microscopy},
   language = {eng},
   issn = {0048-9697},
   journal = {Science of the Total Environment},
   title = {Do foodborne polyethylene microparticles affect the health of rainbow trout (Oncorhynchus mykiss)?},
   url = {https://www.sciencedirect.com/science/article/pii/S0048969721035622?via%3Dihub},
   volume = {793},
   year = {2021}
}

TY  - JOUR
ID  - 1792157
AU  - Hodkovicova, N. - Hollerova, A. - Caloudova, H. - Blahova, J. - Franc, Aleš - Garajová, Michaela - Lenz, J. - Tichy, F. - Faldyna, M. - Kulich, P. - Mares, J. - Machat, R. - Enevova, V. - Svobodova, Z.
PY  - 2021
TI  - Do foodborne polyethylene microparticles affect the health of rainbow trout (Oncorhynchus mykiss)?
JF  - Science of the Total Environment
VL  - 793
IS  - 148490
SP  - 1-14
EP  - 1-14
PB  - Elsevier Science
SN  - 00489697
KW  - Salmonidae
KW  - Antioxidant capacity
KW  - Plastic
KW  - Immunity
KW  - Toxicology
KW  - Electron microscopy
UR  - https://www.sciencedirect.com/science/article/pii/S0048969721035622?via%3Dihub
N2  - Due to the fact that plastic pollution is a global environmental problem of modern age, studies on the impact of these synthetic materials on aquatic, and especially fish organisms, are an important part of the ecosystem and human nutrition. In our study, the toxicity of pristine polyethylene (PE) microparticles (approx. 50 mu m) on rainbow trout (Oncorhynchus mykiss) was tested in three different dietary concentrations - 0.5%, 2% and 5%. After six weeks of exposure, various health indices were evaluated. Electron microscopy of the intestine revealed the disintegration of PE particles to <5 mu m in size, and thus we concluded that microplastics are able to reach tissues. The haematological profile revealed changes in total red blood cells count and haematocrit (5% PE) which could be associated with spleen congestion observed histologically. The marker of lipid peroxidation was increased in gills suggesting the disruption of balance in antioxidant enzymes capacity and histopathological imaging revealed inflammation in higher PE concentrations. In addition, ammonia was decreased and calcium elevated in biochemical profile, confirming the gill damage. Electron microscopy of the gills showed lesions of lamellae and visible rings around the mucinous cell opening indicating their higher activity. Another injured was the liver tissue, as confirmed by hepatodystrophies and increased expression of pro-inflammatory genes in 2% PE. Impaired innate immunity was confirmed by an increased presence of mucinous cells and a decrease in leukocytes. Kidney damage manifested itself by higher expression of pro-inflammatory cytokines and histopathology. The damage in gills, liver and kidney together correlated with the increased antioxidant capacity of plasma. In conclusion, PE microparticles are able to affect health indices of O. mykiss. The potential problem for aquatic ecosystems and even human consumption should be considered. (c) 2021 Elsevier B.V. All rights reserved.
ER  -

HODKOVICOVA, N., A. HOLLEROVA, H. CALOUDOVA, J. BLAHOVA, Aleš FRANC, Michaela GARAJOVÁ, J. LENZ, F. TICHY, M. FALDYNA, P. KULICH, J. MARES, R. MACHAT, V. ENEVOVA and Z. SVOBODOVA. Do foodborne polyethylene microparticles affect the health of rainbow trout (Oncorhynchus mykiss)? \textit{Science of the Total Environment}. Amsterdam: Elsevier Science, 2021, vol.~793, No~148490, p.~1-14. ISSN~0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2021.148490.

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