DASTYCH, Milan, Frantisek HUBATKA, Pavlina TURANEK-KNOTIGOVA, Josef MASEK, Radek KROUPA, Milan RASKA, Jaroslav TURANEK and Lubomir PROCHAZKA. Overexpression of CD44v8-10 in Colon Polyps-A Possible Key to Early Diagnosis. PATHOLOGY & ONCOLOGY RESEARCH. Lausanne: Frontiers, vol. 27, March 2021, p. 1-11. ISSN 1219-4956. doi:10.3389/pore.2021.614281. 2021.
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Basic information
Original name Overexpression of CD44v8-10 in Colon Polyps-A Possible Key to Early Diagnosis
Authors DASTYCH, Milan (203 Czech Republic, belonging to the institution), Frantisek HUBATKA (203 Czech Republic), Pavlina TURANEK-KNOTIGOVA, Josef MASEK (203 Czech Republic), Radek KROUPA (203 Czech Republic, belonging to the institution), Milan RASKA (203 Czech Republic), Jaroslav TURANEK (203 Czech Republic, guarantor) and Lubomir PROCHAZKA (203 Czech Republic).
Edition PATHOLOGY & ONCOLOGY RESEARCH, Lausanne, Frontiers, 2021, 1219-4956.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30109 Pathology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.874
RIV identification code RIV/00216224:14110/21:00122438
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.3389/pore.2021.614281
UT WoS 000639342800001
Keywords in English CD44 isoforms; colorectal precancerosis; colon polyps; cancer markers; RNA splicing
Tags 14110213, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 24/9/2021 10:23.
Abstract
Background and aims: The majority of colorectal cancers arise from detectable adenomatous or serrated lesions. Here we demonstrate how deregulated alternative splicing of CD44 gene in diseased colon mucosa results in downregulation of standard isoform of CD44 gene (CD44s) and upregulation of variant isoform CD44v8-10. Our aim is to show that upregulation of CD44v8-10 isoform is a possible marker of precancerous lesion in human colon. Methods: We analysed pairs of fresh biopsy specimen of large intestine in a cohort of 50 patients. We studied and compared alternative splicing profile of CD44 gene in colon polyps and adjoined healthy colon mucosa. We performed end-point and qRT PCR, western blotting, IHC staining and flow cytometry analyses. Results: We detected more than five-fold overexpression of CD44v8-10 isoform and almost twenty-fold downregulation of standard isoform CD44s in colon polyps compared to adjoined healthy tissue with p = 0.018 and p < 0.001 in a cohort of 50 patients. Our results also show that aberrant splicing of CD44 occurs in both biologically distinct subtypes of colorectal adenoma possibly in ESRP-1 specific manner. Conclusion: 92% of the colon polyp positive patients overexpressed CD44v8-10 isoform in their colon polyps while only 36% of them had positive fecal occult blood test which is currently a standard non-invasive screening technique. Impact: We believe that our results are important for further steps leading to application of CD44v8-10 isoform as a biomarker of colorectal precancerosis in non-invasive detection. Early detection of colon precancerosis means successful prevention of colorectal carcinoma.
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