Effect of Particle Size and Surface Chemistry of
Photon-Upconversion Nanoparticles on Analog and Digital
Immunoassays for Cardiac Troponin

J 2021

Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

BRANDMEIER, Julian, Kirsti RAIKO, Zdeněk FARKA, Riikka PELTOMAA, Matthias Jürgen MICKERT et. al.

Basic information

Original name

Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Authors

BRANDMEIER, Julian (276 Germany), Kirsti RAIKO (246 Finland), Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution), Riikka PELTOMAA (246 Finland), Matthias Jürgen MICKERT (276 Germany), Antonín HLAVÁČEK (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Tero SOUKKA and Hans-Heiner GORRIS (276 Germany)

Edition

Advanced Healthcare Materials, Wiley-VCH, 2021, 2192-2640

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 11.092

RIV identification code

RIV/00216224:14310/21:00119240

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/adhm.202100506

UT WoS

000673454600001

Keywords in English

anti-Stokes emission; cardiac arrest; lanthadine-dopoed nanomaterials; single-molecule immunoassay; troponin; photon-upconversion; upconversion-linked immunosorbent assay

Abstract

V originále

Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL−1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.

Links

GA21-03156S, research and development project

Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

LTAB19011, research and development project

Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION

90127, large research infrastructures

Name: CIISB II

Citovat

BRANDMEIER, Julian, Kirsti RAIKO, Zdeněk FARKA, Riikka PELTOMAA, Matthias Jürgen MICKERT, Antonín HLAVÁČEK, Petr SKLÁDAL, Tero SOUKKA and Hans-Heiner GORRIS. Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin. Advanced Healthcare Materials. Wiley-VCH, 2021, vol. 10, No 18, p. 1-9. ISSN 2192-2640. Available from: https://dx.doi.org/10.1002/adhm.202100506.

@article{1794867,
   author = {Brandmeier, Julian and Raiko, Kirsti and Farka, Zdeněk and Peltomaa, Riikka and Mickert, Matthias Jürgen and Hlaváček, Antonín and Skládal, Petr and Soukka, Tero and Gorris, HansandHeiner},
   article_number = {18},
   doi = {http://dx.doi.org/10.1002/adhm.202100506},
   keywords = {anti-Stokes emission; cardiac arrest; lanthadine-dopoed nanomaterials; single-molecule immunoassay; troponin; photon-upconversion; upconversion-linked immunosorbent assay},
   language = {eng},
   issn = {2192-2640},
   journal = {Advanced Healthcare Materials},
   title = {Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/adhm.202100506},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1794867
AU  - Brandmeier, Julian - Raiko, Kirsti - Farka, Zdeněk - Peltomaa, Riikka - Mickert, Matthias Jürgen - Hlaváček, Antonín - Skládal, Petr - Soukka, Tero - Gorris, Hans-Heiner
PY  - 2021
TI  - Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin
JF  - Advanced Healthcare Materials
VL  - 10
IS  - 18
SP  - 1-9
EP  - 1-9
PB  - Wiley-VCH
SN  - 21922640
KW  - anti-Stokes emission
KW  - cardiac arrest
KW  - lanthadine-dopoed nanomaterials
KW  - single-molecule immunoassay
KW  - troponin
KW  - photon-upconversion
KW  - upconversion-linked immunosorbent assay
UR  - https://onlinelibrary.wiley.com/doi/10.1002/adhm.202100506
N2  - Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL−1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.
ER  -

BRANDMEIER, Julian, Kirsti RAIKO, Zdeněk FARKA, Riikka PELTOMAA, Matthias Jürgen MICKERT, Antonín HLAVÁČEK, Petr SKLÁDAL, Tero SOUKKA and Hans-Heiner GORRIS. Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin. \textit{Advanced Healthcare Materials}. Wiley-VCH, 2021, vol.~10, No~18, p.~1-9. ISSN~2192-2640. Available from: https://dx.doi.org/10.1002/adhm.202100506.

Detailed Information on Publication Record