Centromere Size Scales With Genome Size Across Eukaryotes

J 2021

Centromere Size Scales With Genome Size Across Eukaryotes

PLAČKOVÁ, Klára, Petr BUREŠ and František ZEDEK

Basic information

Original name

Centromere Size Scales With Genome Size Across Eukaryotes

Authors

PLAČKOVÁ, Klára (203 Czech Republic, belonging to the institution), Petr BUREŠ (203 Czech Republic, belonging to the institution) and František ZEDEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific Reports, London, Nature Research, 2021, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10602 Biology , Evolutionary biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.996

RIV identification code

RIV/00216224:14310/21:00119242

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-021-99386-7

UT WoS

000706380800025

Keywords in English

Eukaryotes; centromere size; CenH3; kinetochore size

Abstract

V originále

Previous studies on grass species suggested that the total centromere size (sum of all centromere sizes in a cell) may be determined by the genome size, possibly because stable scaling is important for proper cell division. However, it is unclear whether this relationship is universal. Here we analyze the total centromere size using the CenH3-immunofluorescence area as a proxy in 130 taxa including plants, animals, fungi, and protists. We verified the reliability of our methodological approach by comparing our measurements with available ChIP-seq-based measurements of the size of CenH3- binding domains. Data based on these two independent methods showed the same positive relationship between the total centromere size and genome size. Our results demonstrate that the genome size is a strong predictor (R-squared= 0.964) of the total centromere size universally across Eukaryotes. We also show that this relationship is independent of phylogenetic relatedness and centromere type (monocentric, metapolycentric, and holocentric), implying a common mechanism maintaining stable total centromere size in Eukaryotes.

Links

GA20-15989S, research and development project

Name: Evoluce velikosti genomu - centromerický drajv v nové roli (Acronym: Centrogenomtah)

Investor: Czech Science Foundation

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PLAČKOVÁ, Klára, Petr BUREŠ and František ZEDEK. Centromere Size Scales With Genome Size Across Eukaryotes. Scientific Reports. London: Nature Research, 2021, vol. 11, No 1, p. 19811-19817. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-99386-7.

@article{1795398,
   author = {Plačková, Klára and Bureš, Petr and Zedek, František},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-021-99386-7},
   keywords = {Eukaryotes; centromere size; CenH3; kinetochore size},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Centromere Size Scales With Genome Size Across Eukaryotes},
   url = {https://doi.org/10.1038/s41598-021-99386-7},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1795398
AU  - Plačková, Klára - Bureš, Petr - Zedek, František
PY  - 2021
TI  - Centromere Size Scales With Genome Size Across Eukaryotes
JF  - Scientific Reports
VL  - 11
IS  - 1
SP  - 19811
EP  - 19811
PB  - Nature Research
SN  - 20452322
KW  - Eukaryotes
KW  - centromere size
KW  - CenH3
KW  - kinetochore size
UR  - https://doi.org/10.1038/s41598-021-99386-7
N2  - Previous studies on grass species suggested that the total centromere size (sum of all centromere sizes in a cell) may be determined by the genome size, possibly because stable scaling is important for proper cell division. However, it is unclear whether this relationship is universal. Here we analyze the total centromere size using the CenH3-immunofluorescence area as a proxy in 130 taxa including plants, animals, fungi, and protists. We verified the reliability of our methodological approach by comparing our measurements with available ChIP-seq-based measurements of the size of CenH3- binding domains. Data based on these two independent methods showed the same positive relationship between the total centromere size and genome size. Our results demonstrate that the genome size is a strong predictor (R-squared= 0.964) of the total centromere size universally across Eukaryotes. We also show that this relationship is independent of phylogenetic relatedness and centromere type (monocentric, metapolycentric, and holocentric), implying a common mechanism maintaining stable total centromere size in Eukaryotes.
ER  -

PLAČKOVÁ, Klára, Petr BUREŠ and František ZEDEK. Centromere Size Scales With Genome Size Across Eukaryotes. \textit{Scientific Reports}. London: Nature Research, 2021, vol.~11, No~1, p.~19811-19817. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-99386-7.

Detailed Information on Publication Record