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@article{1795398, author = {Plačková, Klára and Bureš, Petr and Zedek, František}, article_location = {London}, article_number = {1}, doi = {http://dx.doi.org/10.1038/s41598-021-99386-7}, keywords = {Eukaryotes; centromere size; CenH3; kinetochore size}, language = {eng}, issn = {2045-2322}, journal = {Scientific Reports}, title = {Centromere Size Scales With Genome Size Across Eukaryotes}, url = {https://doi.org/10.1038/s41598-021-99386-7}, volume = {11}, year = {2021} }
TY - JOUR ID - 1795398 AU - Plačková, Klára - Bureš, Petr - Zedek, František PY - 2021 TI - Centromere Size Scales With Genome Size Across Eukaryotes JF - Scientific Reports VL - 11 IS - 1 SP - 19811 EP - 19811 PB - Nature Research SN - 20452322 KW - Eukaryotes KW - centromere size KW - CenH3 KW - kinetochore size UR - https://doi.org/10.1038/s41598-021-99386-7 N2 - Previous studies on grass species suggested that the total centromere size (sum of all centromere sizes in a cell) may be determined by the genome size, possibly because stable scaling is important for proper cell division. However, it is unclear whether this relationship is universal. Here we analyze the total centromere size using the CenH3-immunofluorescence area as a proxy in 130 taxa including plants, animals, fungi, and protists. We verified the reliability of our methodological approach by comparing our measurements with available ChIP-seq-based measurements of the size of CenH3- binding domains. Data based on these two independent methods showed the same positive relationship between the total centromere size and genome size. Our results demonstrate that the genome size is a strong predictor (R-squared= 0.964) of the total centromere size universally across Eukaryotes. We also show that this relationship is independent of phylogenetic relatedness and centromere type (monocentric, metapolycentric, and holocentric), implying a common mechanism maintaining stable total centromere size in Eukaryotes. ER -
PLAČKOVÁ, Klára, Petr BUREŠ and František ZEDEK. Centromere Size Scales With Genome Size Across Eukaryotes. \textit{Scientific Reports}. London: Nature Research, 2021, vol.~11, No~1, p.~19811-19817. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-021-99386-7.
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