Substantial intraspecific genome size variation in golden-brown
algae and its phenotypic consequences

J 2020

Substantial intraspecific genome size variation in golden-brown algae and its phenotypic consequences

CERTNEROVA, Dora and Pavel SKALOUD

Basic information

Original name

Substantial intraspecific genome size variation in golden-brown algae and its phenotypic consequences

Authors

CERTNEROVA, Dora (203 Czech Republic) and Pavel SKALOUD (203 Czech Republic)

Edition

Annals of Botany, Oxford, Oxford University Press, 2020, 0305-7364

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.357

RIV identification code

RIV/00216224:14310/20:00122492

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1093/aob/mcaa133

UT WoS

000591876700010

Keywords in English

Intraspecific DNA content variation; genome size; flow cytometry; golden-brown algae; Synura petersenii; GC content; biovolume; growth rate; environmental conditions; ITS

Abstract

V originále

Background and Aims While nuclear DNA content variation and its phenotypic consequences have been well described for animals, vascular plants and macroalgae, much less about this topic is known regarding unicellular algae and protists in general. The dearth of data is especially pronounced when it comes to intraspecific genome size variation. This study attempts to investigate the extent of intraspecific variability in genome size and its adaptive consequences in a microalgal species. Methods Propidium iodide flow cytometry was used to estimate the absolute genome size of 131 strains (isolates) of the golden-brown alga Synura petersenii (Chrysophyceae, Stramenopiles), identified by identical internal transcribed spacer (ITS) rDNA barcodes. Cell size, growth rate and genomic GC content were further assessed on a sub-set of strains. Geographic location of 67 sampling sites across the Northern hemisphere was used to extract climatic database data and to evaluate the ecogeographical distribution of genome size diversity. Key Results Genome size ranged continuously from 0.97 to 2.02 pg of DNA across the investigated strains. The genome size was positively associated with cell size and negatively associated with growth rate. Bioclim variables were not correlated with genome size variation. No clear trends in the geographical distribution of strains of a particular genome size were detected, and strains of different genome size occasionally coexisted at the same locality. Genomic GC content was significantly associated only with genome size via a quadratic relationship. Conclusions Genome size variability in S. petersenii was probably triggered by an evolutionary mechanism operating via gradual changes in genome size accompanied by changes in genomic GC content, such as. for example, proliferation of transposable elements. The variation was reflected in cell size and relative growth rate, possibly with adaptive consequences.

Links

90051, large research infrastructures

Name: RECETOX II

Citovat

CERTNEROVA, Dora and Pavel SKALOUD. Substantial intraspecific genome size variation in golden-brown algae and its phenotypic consequences. Annals of Botany. Oxford: Oxford University Press, 2020, vol. 126, No 6, p. 1077-1087. ISSN 0305-7364. Available from: https://dx.doi.org/10.1093/aob/mcaa133.

@article{1795417,
   author = {Certnerova, Dora and Skaloud, Pavel},
   article_location = {Oxford},
   article_number = {6},
   doi = {http://dx.doi.org/10.1093/aob/mcaa133},
   keywords = {Intraspecific DNA content variation; genome size; flow cytometry; golden-brown algae; Synura petersenii; GC content; biovolume; growth rate; environmental conditions; ITS},
   language = {eng},
   issn = {0305-7364},
   journal = {Annals of Botany},
   title = {Substantial intraspecific genome size variation in golden-brown algae and its phenotypic consequences},
   url = {https://academic.oup.com/aob/article-abstract/126/6/1077/5873694?redirectedFrom=fulltext},
   volume = {126},
   year = {2020}
}

TY  - JOUR
ID  - 1795417
AU  - Certnerova, Dora - Skaloud, Pavel
PY  - 2020
TI  - Substantial intraspecific genome size variation in golden-brown algae and its phenotypic consequences
JF  - Annals of Botany
VL  - 126
IS  - 6
SP  - 1077-1087
EP  - 1077-1087
PB  - Oxford University Press
SN  - 03057364
KW  - Intraspecific DNA content variation
KW  - genome size
KW  - flow cytometry
KW  - golden-brown algae
KW  - Synura petersenii
KW  - GC content
KW  - biovolume
KW  - growth rate
KW  - environmental conditions
KW  - ITS
UR  - https://academic.oup.com/aob/article-abstract/126/6/1077/5873694?redirectedFrom=fulltext
N2  - Background and Aims While nuclear DNA content variation and its phenotypic consequences have been well described for animals, vascular plants and macroalgae, much less about this topic is known regarding unicellular algae and protists in general. The dearth of data is especially pronounced when it comes to intraspecific genome size variation. This study attempts to investigate the extent of intraspecific variability in genome size and its adaptive consequences in a microalgal species. Methods Propidium iodide flow cytometry was used to estimate the absolute genome size of 131 strains (isolates) of the golden-brown alga Synura petersenii (Chrysophyceae, Stramenopiles), identified by identical internal transcribed spacer (ITS) rDNA barcodes. Cell size, growth rate and genomic GC content were further assessed on a sub-set of strains. Geographic location of 67 sampling sites across the Northern hemisphere was used to extract climatic database data and to evaluate the ecogeographical distribution of genome size diversity. Key Results Genome size ranged continuously from 0.97 to 2.02 pg of DNA across the investigated strains. The genome size was positively associated with cell size and negatively associated with growth rate. Bioclim variables were not correlated with genome size variation. No clear trends in the geographical distribution of strains of a particular genome size were detected, and strains of different genome size occasionally coexisted at the same locality. Genomic GC content was significantly associated only with genome size via a quadratic relationship. Conclusions Genome size variability in S. petersenii was probably triggered by an evolutionary mechanism operating via gradual changes in genome size accompanied by changes in genomic GC content, such as. for example, proliferation of transposable elements. The variation was reflected in cell size and relative growth rate, possibly with adaptive consequences.
ER  -

CERTNEROVA, Dora and Pavel SKALOUD. Substantial intraspecific genome size variation in golden-brown algae and its phenotypic consequences. \textit{Annals of Botany}. Oxford: Oxford University Press, 2020, vol.~126, No~6, p.~1077-1087. ISSN~0305-7364. Available from: https://dx.doi.org/10.1093/aob/mcaa133.

Detailed Information on Publication Record