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@article{1796018,
author = {Szklanny, Ariel A. and Machour, Majd and Redenski, Idan and Chochola, Václav and Goldfracht, Idit and Kaplan, Ben and Epshtein, Mark and Yameen, Haneen Simaan and Merdler, Uri and Feinberg, Adam and Seliktar, Dror and Korin, Netanel and Jaroš, Josef and Levenberg, Shulamit},
article_location = {WEINHEIM},
article_number = {42},
doi = {http://dx.doi.org/10.1002/adma.202102661},
keywords = {3D bioprinting; ECM bioink; engineered flap; personalized medicine; tissue engineering; vascularization.},
language = {eng},
issn = {0935-9648},
journal = {ADVANCED MATERIALS},
title = {3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion},
url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/adma.202102661},
volume = {33},
year = {2021}
}
TY - JOUR
ID - 1796018
AU - Szklanny, Ariel A. - Machour, Majd - Redenski, Idan - Chochola, Václav - Goldfracht, Idit - Kaplan, Ben - Epshtein, Mark - Yameen, Haneen Simaan - Merdler, Uri - Feinberg, Adam - Seliktar, Dror - Korin, Netanel - Jaroš, Josef - Levenberg, Shulamit
PY - 2021
TI - 3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion
JF - ADVANCED MATERIALS
VL - 33
IS - 42
SP - 1-19
EP - 1-19
PB - WILEY-V C H VERLAG GMBH
SN - 09359648
KW - 3D bioprinting
KW - ECM bioink
KW - engineered flap
KW - personalized medicine
KW - tissue engineering
KW - vascularization.
UR - https://onlinelibrary.wiley.com/doi/epdf/10.1002/adma.202102661
N2 - Engineering hierarchical vasculatures is critical for creating implantable functional thick tissues. Current approaches focus on fabricating mesoscale vessels for implantation or hierarchical microvascular in vitro models, but a combined approach is yet to be achieved to create engineered tissue flaps. Here, millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues interconnect, creating fully engineered hierarchical vascular constructs for implantation. Endothelial and support cells spontaneously form microvascular networks in bioprinted tissues using a human collagen bioink. Sacrificial molds are used to create polymeric vessel-like scaffolds and endothelial cells seeded in their lumen form native-like endothelia. Assembling endothelialized scaffolds within vascularizing hydrogels incites the bioprinted vasculature and endothelium to cooperatively create vessels, enabling tissue perfusion through the scaffold lumen. Using a cuffing microsurgery approach, the engineered tissue is directly anastomosed with a rat femoral artery, promoting a rich host vasculature within the implanted tissue. After two weeks in vivo, contrast microcomputer tomography imaging and lectin perfusion of explanted engineered tissues verify the host ingrowth vasculature's functionality. Furthermore, the hierarchical vessel network (VesselNet) supports in vitro functionality of cardiomyocytes. Finally, the proposed approach is expanded to mimic complex structures with native-like millimetric vessels. This work presents a novel strategy aiming to create fully-engineered patient-specific thick tissue flaps.
ER -
SZKLANNY, Ariel A., Majd MACHOUR, Idan REDENSKI, Václav CHOCHOLA, Idit GOLDFRACHT, Ben KAPLAN, Mark EPSHTEIN, Haneen Simaan YAMEEN, Uri MERDLER, Adam FEINBERG, Dror SELIKTAR, Netanel KORIN, Josef JAROŠ and Shulamit LEVENBERG. 3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion. \textit{ADVANCED MATERIALS}. WEINHEIM: WILEY-V C H VERLAG GMBH, 2021, vol.~33, No~42, p.~1-19. ISSN~0935-9648. Available from: https://dx.doi.org/10.1002/adma.202102661.
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