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@article{1796119,
author = {Vítězová, Monika and Lochman, Jan and Zapletalová, Martina and Ratering, Stefan and Schnell, Sylvia and Vítěz, Tomáš},
article_number = {11},
doi = {http://dx.doi.org/10.1007/s11274-021-03152-w},
keywords = {Anaerobic digestion; Biogas; mcrA primers; Methanogenic Archaea; Next-Generation Sequencing; Temperature},
language = {eng},
issn = {0959-3993},
journal = {World Journal of Microbiology and Biotechnology},
title = {Archaeal community dynamics in biogas fermentation at various temperatures assessed by mcrA amplicon sequencing using different primer pairs},
url = {https://doi.org/10.1007/s11274-021-03152-w},
volume = {37},
year = {2021}
}
TY - JOUR
ID - 1796119
AU - Vítězová, Monika - Lochman, Jan - Zapletalová, Martina - Ratering, Stefan - Schnell, Sylvia - Vítěz, Tomáš
PY - 2021
TI - Archaeal community dynamics in biogas fermentation at various temperatures assessed by mcrA amplicon sequencing using different primer pairs
JF - World Journal of Microbiology and Biotechnology
VL - 37
IS - 11
SP - 188
EP - 188
PB - Springer
SN - 09593993
KW - Anaerobic digestion
KW - Biogas
KW - mcrA primers
KW - Methanogenic Archaea
KW - Next-Generation Sequencing
KW - Temperature
UR - https://doi.org/10.1007/s11274-021-03152-w
N2 - In this study, the taxonomic and functional diversity of methanogenic archaea in two parallel 120 l fermenters operated at different temperatures and fed with maize silage was estimated by mcrA metabarcoding analysis using two typical primer pairs (ML and MLA) amplifying part of the functional methyl coenzyme M reductase (mcrA) gene. The alpha diversity indices showed that the ML primer pair detected a higher Operational Taxonomic Unit (OTU) abundance compared to the MLA primer pair and methanogen diversity was significantly lower in the 60 °C fermenters. The beta diversity analysis showed the methanogenic community clustered together at 50 °C and 40° and was statistically different from the 60 °C community. Similar, to alpha diversity, beta diversity was also significantly different between primer pairs. At all temperatures analysed, the primer pairs showed a different abundance of the different methanogenic OTUs, e.g. more OTUs relative to Methanoculleus sp. with the ML primer pair, and more OTUs corresponding to Methanobacterium sp. with the MLA primer pair. Moreover, OTUs corresponding to Methanosphaera sp. and Methanobrevibacter sp. were found only by using ML primer pair, while the MLA primer pair detected sequences corresponding to Methanothrix sp.
ER -
VÍTĚZOVÁ, Monika, Jan LOCHMAN, Martina ZAPLETALOVÁ, Stefan RATERING, Sylvia SCHNELL and Tomáš VÍTĚZ. Archaeal community dynamics in biogas fermentation at various temperatures assessed by mcrA amplicon sequencing using different primer pairs. \textit{World Journal of Microbiology and Biotechnology}. Springer, 2021, vol.~37, No~11, p.~188-198. ISSN~0959-3993. Available from: https://dx.doi.org/10.1007/s11274-021-03152-w.
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