Detailed Information on Publication Record
2021
Derivation and characterization of clinical-grade human embryonic stem cells
SOURALOVÁ, Tereza, Zuzana HOLUBCOVÁ, Drahomíra KYJOVSKÁ, Aleš HAMPL, Irena KOUTNÁ et. al.Basic information
Original name
Derivation and characterization of clinical-grade human embryonic stem cells
Authors
SOURALOVÁ, Tereza (203 Czech Republic, guarantor, belonging to the institution), Zuzana HOLUBCOVÁ (203 Czech Republic, belonging to the institution), Drahomíra KYJOVSKÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Irena KOUTNÁ (203 Czech Republic, belonging to the institution)
Edition
XII. international conference „Bioimplantologie 2021“, Mikulov, Czech Republic, 2021
Other information
Language
English
Type of outcome
Prezentace na konferencích
Field of Study
10601 Cell biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/21:00120147
Organization unit
Faculty of Medicine
ISBN
978-80-01-06886-1
Keywords in English
clinical-grade human embryonic stem cells; derivation and characterization
Tags
Tags
International impact, Reviewed
Změněno: 31/5/2023 12:44, Mgr. Tereza Souralová
Abstract
V originále
Human embryonic stem cells (hESCs) represent an unlimited source of cells for the differentiation into the derivates that are suitable for a variety of biomedical applications. Cell therapies, disease modeling, and drug testing, which are examples of application, demand robust and replicable protocols for the derivation and differentiation of hESCs. Nevertheless, allogeneic background and undefined culture conditions are limitations of current protocols. Therefore, here we present the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos (excess for fertility treatment) under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinicalgrade hESC lines for a variety of clinical applications.
Links
| NV18-08-00412, research and development project |
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